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Protein

Diphthamide biosynthesis protein 1

Gene

Dph1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). Acts also as a tumor suppressor in lung and breast cancers. Plays a role in embryonic growth, organogenesis and postnatal survival. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells (By similarity).By similarity2 Publications

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

GO - Biological processi

  • cell proliferation Source: MGI
  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: GO_Central
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthamide biosynthesis protein 1
Alternative name(s):
DPH1 homolog
Short name:
MmDph1
Diphthamide biosynthesis protein 2 homolog-like 1
Short name:
DPH2-like 1
Ovarian cancer-associated gene 1 protein homolog
Gene namesi
Name:Dph1
Synonyms:Dph2l1, Ovca1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2151233. Dph1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Diphthamide biosynthesis protein 1PRO_0000307883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei418 – 4181PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5NCQ5.
MaxQBiQ5NCQ5.
PaxDbiQ5NCQ5.
PeptideAtlasiQ5NCQ5.
PRIDEiQ5NCQ5.

PTM databases

iPTMnetiQ5NCQ5.
PhosphoSiteiQ5NCQ5.

Expressioni

Tissue specificityi

Strongly expressed in kidney and liver. Moderately expressed in brain, skin and testis. Weakly expressed in heart, lung, small intestine, spleen, stomach and thymus.1 Publication

Developmental stagei

Expressed in embryos from 8.5 to 10.5 dpc. Expressed in lung, stomach, brain, heart, kidney and gonads at 14.5 dpc.1 Publication

Gene expression databases

BgeeiQ5NCQ5.
CleanExiMM_DPH1.
ExpressionAtlasiQ5NCQ5. baseline and differential.
GenevisibleiQ5NCQ5. MM.

Interactioni

Subunit structurei

Interacts with RBM8A (By similarity). Interacts with DPH2.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dph2Q9CR252EBI-1561119,EBI-1561134

Protein-protein interaction databases

IntActiQ5NCQ5. 1 interaction.
STRINGi10090.ENSMUSP00000042162.

Structurei

3D structure databases

ProteinModelPortaliQ5NCQ5.
SMRiQ5NCQ5. Positions 56-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DPH1/DPH2 family. DPH1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2648. Eukaryota.
COG1736. LUCA.
GeneTreeiENSGT00550000075112.
HOGENOMiHOG000164743.
HOVERGENiHBG107822.
InParanoidiQ5NCQ5.
KOiK07561.
OMAiTIWRIRE.
OrthoDBiEOG7VX8VX.
PhylomeDBiQ5NCQ5.
TreeFamiTF105746.

Family and domain databases

InterProiIPR016435. DPH1/DPH2.
[Graphical view]
PANTHERiPTHR10762. PTHR10762. 1 hit.
PfamiPF01866. Diphthamide_syn. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00322. diphth2_R. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5NCQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALVVSETA EPGSRVGPGR GRISRGRLAN QIPPEVLNNP QLQAAVQVLP
60 70 80 90 100
SNYNFEIPKT IWRIQQAQAK KVALQMPEGL LLFACTIVDI LERFTEAEVM
110 120 130 140 150
VMGDVTYGAC CVDDFTARAL GVDFLVHYGH SCLVPMDTSV QDFRVLYVFV
160 170 180 190 200
DIRIDTAHLL DSVRLTFTPG SSLALVSTIQ FVSTLQAAAQ ELKADYHISV
210 220 230 240 250
PQCKPLSPGE ILGCTSPRLS KEVEAVVYLG DGRFHLESVM IANPNIPAYR
260 270 280 290 300
YDPYGKVLSR EYYDHQRMQA TRQEAIAAAR SAKSWGLILG TLGRQGSPKI
310 320 330 340 350
LEHLESQLRN LGLPFVRLLL SEIFPSKLSL LPEVDVWVQV ACPRLSIDWG
360 370 380 390 400
SAFPKPLLTP YEAAVALKDI SWQQPYPMDF YSGSSLGPWT VNYGRDRAPR
410 420 430
GLCQPASDKV QQGSRGGSPA PACESCNCAD QKATSPAP
Length:438
Mass (Da):48,004
Last modified:February 1, 2005 - v1
Checksum:i07CA505B08F72AB7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981E → K in AAM14629 (PubMed:11527402).Curated
Sequence conflicti208 – 2081P → L in AAM14629 (PubMed:11527402).Curated
Sequence conflicti321 – 3211S → P in AAM14629 (PubMed:11527402).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY078170 mRNA. Translation: AAM14629.1.
AL603905 Genomic DNA. Translation: CAI35090.1.
BC044663 mRNA. Translation: AAH44663.1.
CCDSiCCDS25042.1.
PIRiJC7758.
RefSeqiNP_652762.2. NM_144491.2.
UniGeneiMm.41496.

Genome annotation databases

EnsembliENSMUST00000044949; ENSMUSP00000042162; ENSMUSG00000078789.
GeneIDi116905.
KEGGimmu:116905.
UCSCiuc007kdg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY078170 mRNA. Translation: AAM14629.1.
AL603905 Genomic DNA. Translation: CAI35090.1.
BC044663 mRNA. Translation: AAH44663.1.
CCDSiCCDS25042.1.
PIRiJC7758.
RefSeqiNP_652762.2. NM_144491.2.
UniGeneiMm.41496.

3D structure databases

ProteinModelPortaliQ5NCQ5.
SMRiQ5NCQ5. Positions 56-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5NCQ5. 1 interaction.
STRINGi10090.ENSMUSP00000042162.

PTM databases

iPTMnetiQ5NCQ5.
PhosphoSiteiQ5NCQ5.

Proteomic databases

EPDiQ5NCQ5.
MaxQBiQ5NCQ5.
PaxDbiQ5NCQ5.
PeptideAtlasiQ5NCQ5.
PRIDEiQ5NCQ5.

Protocols and materials databases

DNASUi116905.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044949; ENSMUSP00000042162; ENSMUSG00000078789.
GeneIDi116905.
KEGGimmu:116905.
UCSCiuc007kdg.1. mouse.

Organism-specific databases

CTDi1801.
MGIiMGI:2151233. Dph1.

Phylogenomic databases

eggNOGiKOG2648. Eukaryota.
COG1736. LUCA.
GeneTreeiENSGT00550000075112.
HOGENOMiHOG000164743.
HOVERGENiHBG107822.
InParanoidiQ5NCQ5.
KOiK07561.
OMAiTIWRIRE.
OrthoDBiEOG7VX8VX.
PhylomeDBiQ5NCQ5.
TreeFamiTF105746.

Enzyme and pathway databases

UniPathwayiUPA00559.
ReactomeiR-MMU-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

PROiQ5NCQ5.
SOURCEiSearch...

Gene expression databases

BgeeiQ5NCQ5.
CleanExiMM_DPH1.
ExpressionAtlasiQ5NCQ5. baseline and differential.
GenevisibleiQ5NCQ5. MM.

Family and domain databases

InterProiIPR016435. DPH1/DPH2.
[Graphical view]
PANTHERiPTHR10762. PTHR10762. 1 hit.
PfamiPF01866. Diphthamide_syn. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00322. diphth2_R. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, structure, and expression of the mouse Ovca1 gene."
    Chen C.-M., Behringer R.R.
    Biochem. Biophys. Res. Commun. 286:1019-1026(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-438.
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Ovca1 regulates cell proliferation, embryonic development, and tumorigenesis."
    Chen C.-M., Behringer R.R.
    Genes Dev. 18:320-332(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2."
    Liu S., Milne G.T., Kuremsky J.G., Fink G.R., Leppla S.H.
    Mol. Cell. Biol. 24:9487-9497(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DPH2.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Liver, Spleen and Testis.

Entry informationi

Entry nameiDPH1_MOUSE
AccessioniPrimary (citable) accession number: Q5NCQ5
Secondary accession number(s): Q80UX6, Q8R445
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: February 1, 2005
Last modified: July 6, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Knockout mice leads to an increased frequency of tumor formation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.