Q5NCP0 (RNF43_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase RNF43
EC=6.3.2.-

Alternative name(s):
RING finger protein 43

Gene names

Name:

Rnf43

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Ref.4
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with AKAP8L, NONO and SFPQ. Interacts with FZD5 By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Nucleus envelope By similarity. Note: May be secreted By similarity.
Tissue specificity	Expressed in crypt base columnar cells of small intestinal epithelium. Crypt base columnar cells are small cycling cells residing between the terminally differentiated Paneth cells at crypt bottoms. Colocalizes with Lgr5-positive stem cells. Ref.4
Post-translational modification	Autoubiquitinated By similarity.
Disruption phenotype	Conditional knockout mice lacking both Rnf43 and Znrf3 in intestine show a marked expansion of the proliferative compartment, resembling the effects of acute deletion of Apc. Ref.4
Sequence similarities	Belongs to the ZNRF3 family. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Wnt signaling pathway
Cellular component	Cell membrane Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Glycoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor catabolic process Inferred from sequence or structural similarity. Source: UniProtKB Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB stem cell proliferation Inferred from mutant phenotype Ref.4. Source: UniProtKB
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5NCP0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5NCP0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: Missing.

Isoform 3 (identifier: Q5NCP0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     112-116: PRRAP → QRSTL
     117-784: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 784	761	E3 ubiquitin-protein ligase RNF43		PRO_0000278240
Regions
	Topological domain	24 – 197	174	Extracellular Potential
	Transmembrane	198 – 218	21	Helical; Potential
	Topological domain	219 – 784	566	Cytoplasmic Potential
	Zinc finger	272 – 313	42	RING-type; atypical
	Compositional bias	443 – 503	61	Ser-rich
	Compositional bias	547 – 557	11	His-rich
	Compositional bias	569 – 741	173	Pro-rich
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 127	127	Missing in isoform 2.		VSP_023203
	Alternative sequence	112 – 116	5	PRRAP → QRSTL in isoform 3.		VSP_023204
	Alternative sequence	117 – 784	668	Missing in isoform 3.		VSP_023205
Experimental info
	Sequence conflict	367	1	A → S in AAH75707. Ref.3
	Sequence conflict	631	1	P → PSP in AAH75707. Ref.3
	Sequence conflict	755	1	G → D in AAH75707. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 53D4DBE8EE43C755	FASTA	784	86,304
	10 20 30 40 50 60 MSGGHQLQLA VLWPWLLMAT LHAGFGHTGR VLAAAVESER SAEQKAVIRV IPLKMDPTGK 70 80 90 100 110 120 LNLTLEGVFA GVAEVTPAEG KLMQSHPLYL CNASDDDNLE PGFISIVKLE SPRRAPRPCL 130 140 150 160 170 180 SLASKARMAG ERGANAVLFD ITEDRSAAEQ LQQPLGLTKP VVLIWGSDAA KLMEFVYKNR 190 200 210 220 230 240 KAYVWIELKE PPAGANYDVW ILLTVVGTVF VIILASVLRI RCRPHHSRPD PLQQRTARAI 250 260 270 280 290 300 SQLATRRYQA GCRRARAEWP DSGSSCSSTP VCAICLEEFS EGQELRVISC LHEFHRTCVD 310 320 330 340 350 360 PWLYQHRTCP LCMFNIVEGD SFSQAPAASP SYQEPGRRLH LIRQHPGHAH YHLPSAYLLG 370 380 390 400 410 420 PSRTSVARTP RPRPFLPSQE PSMGSRHQRL PRTSHLRAPE EQQHLAVSPH PYAQGWGLNR 430 440 450 460 470 480 LRCTSQHPAA CPVALRRARP HESSGSGESY CTERSGYLAD GPASDSSSGP CHGSSSDSVV 490 500 510 520 530 540 NCTDVSLQGI HGSSSTFRSS LSSDFDPLVY CSPEGDLQGK GIQPSVTSRP RSLDSVVPRG 550 560 570 580 590 600 ETQVSSHIHY HRHRHHHYKR QFQWHGRKPG PETGIPQSMP AASHTQLEPS LPDQQLITPN 610 620 630 640 650 660 PTASSMLPNP QRPRALTEPA PGLAEASSPS PSPKPNPSGL LNLQKSSLTV RHPHRKRRGG 670 680 690 700 710 720 PSEPLPTSLP PDLTVHTACP VFPHYSPRLA YPWPPEVHPL MFRPPGPDRR LLHEVPGPCY 730 740 750 760 770 780 SSSQPVWLYL NPCQPLGPCL PGEGHSKWTF DSPEGRRCPY SHCQVLPAQP GSEEELEELC EQAV
	Isoform 2 [UniParc]. Checksum: 49EC496C3981A722	FASTA	657	72,655
	10 20 30 40 50 60 MAGERGANAV LFDITEDRSA AEQLQQPLGL TKPVVLIWGS DAAKLMEFVY KNRKAYVWIE 70 80 90 100 110 120 LKEPPAGANY DVWILLTVVG TVFVIILASV LRIRCRPHHS RPDPLQQRTA RAISQLATRR 130 140 150 160 170 180 YQAGCRRARA EWPDSGSSCS STPVCAICLE EFSEGQELRV ISCLHEFHRT CVDPWLYQHR 190 200 210 220 230 240 TCPLCMFNIV EGDSFSQAPA ASPSYQEPGR RLHLIRQHPG HAHYHLPSAY LLGPSRTSVA 250 260 270 280 290 300 RTPRPRPFLP SQEPSMGSRH QRLPRTSHLR APEEQQHLAV SPHPYAQGWG LNRLRCTSQH 310 320 330 340 350 360 PAACPVALRR ARPHESSGSG ESYCTERSGY LADGPASDSS SGPCHGSSSD SVVNCTDVSL 370 380 390 400 410 420 QGIHGSSSTF RSSLSSDFDP LVYCSPEGDL QGKGIQPSVT SRPRSLDSVV PRGETQVSSH 430 440 450 460 470 480 IHYHRHRHHH YKRQFQWHGR KPGPETGIPQ SMPAASHTQL EPSLPDQQLI TPNPTASSML 490 500 510 520 530 540 PNPQRPRALT EPAPGLAEAS SPSPSPKPNP SGLLNLQKSS LTVRHPHRKR RGGPSEPLPT 550 560 570 580 590 600 SLPPDLTVHT ACPVFPHYSP RLAYPWPPEV HPLMFRPPGP DRRLLHEVPG PCYSSSQPVW 610 620 630 640 650 LYLNPCQPLG PCLPGEGHSK WTFDSPEGRR CPYSHCQVLP AQPGSEEELE ELCEQAV
	Isoform 3 [UniParc]. Checksum: 27523548EAABED79	FASTA	116	12,491
	10 20 30 40 50 60 MSGGHQLQLA VLWPWLLMAT LHAGFGHTGR VLAAAVESER SAEQKAVIRV IPLKMDPTGK 70 80 90 100 110 LNLTLEGVFA GVAEVTPAEG KLMQSHPLYL CNASDDDNLE PGFISIVKLE SQRSTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6J. Tissue: Skin and Wolffian duct.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 84-784 (ISOFORM 1). Strain: Czech II and FVB/N. Tissue: Mammary tumor.
[4]	"Tumour suppressor RNF43 is a stem-cell E3 ligase that induces endocytosis of Wnt receptors." Koo B.K., Spit M., Jordens I., Low T.Y., Stange D.E., van de Wetering M., van Es J.H., Mohammed S., Heck A.J., Maurice M.M., Clevers H. Nature 488:665-669(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK028750 mRNA. Translation: BAC26097.1. AK032782 mRNA. Translation: BAC28018.1. AL596086, AL604022 Genomic DNA. Translation: CAI35141.1. AL604022, AL596086 Genomic DNA. Translation: CAI35962.1. CU393486 Genomic DNA. Translation: CAQ52071.1. BC029717 mRNA. Translation: AAH29717.1. BC075707 mRNA. Translation: AAH75707.1.
IPI	IPI00272698. IPI00626683. IPI00875921.
RefSeq	NP_766036.2. NM_172448.3.
UniGene	Mm.440230.
3D structure databases
HSSP	HSSP built from PDB template 1IYM based on UniProtKB Q9LRB7.
ProteinModelPortal	Q5NCP0.
SMR	Q5NCP0. Positions 272-317.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59915N.
STRING	10090.ENSMUSP00000090476.
Proteomic databases
PRIDE	Q5NCP0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000040089; ENSMUSP00000044241; ENSMUSG00000034177. ENSMUST00000092800; ENSMUSP00000090476; ENSMUSG00000034177. ENSMUST00000165679; ENSMUSP00000130685; ENSMUSG00000034177.
GeneID	207742.
KEGG	mmu:207742.
UCSC	uc007kue.2. mouse.
Organism-specific databases
CTD	54894.
MGI	MGI:2442609. Rnf43.
Phylogenomic databases
eggNOG	NOG329235.
GeneTree	ENSGT00530000063291.
HOGENOM	HOG000246992.
HOVERGEN	HBG093916.
InParanoid	B2KGH3.
KO	K15694.
OMA	YLLGPSR.
OrthoDB	EOG4N04FJ.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q5NCP0.
Bgee	Q5NCP0.
CleanEx	MM_RNF43.
Genevestigator	Q5NCP0.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF13639. zf-RING_2. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	372023.
SOURCE	Search...

Entry information

Entry name

RNF43_MOUSE

Accession

Primary (citable) accession number: Q5NCP0
Secondary accession number(s): B2KGH3 , Q6DI76, Q8BME0, Q8C191, Q8K0X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program