Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Metabotropic glutamate receptor 6

Gene

Grm6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity (By similarity). Signaling stimulates TRPM1 channel activity and Ca2+ uptake. Required for normal vision.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei148 – 1481GlutamateBy similarity
Binding sitei219 – 2191GlutamateBy similarity
Binding sitei301 – 3011GlutamateBy similarity
Binding sitei394 – 3941GlutamateBy similarity

GO - Molecular functioni

GO - Biological processi

  • detection of light stimulus involved in visual perception Source: UniProtKB
  • G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • locomotory behavior Source: UniProtKB
  • positive regulation of calcium ion import Source: UniProtKB
  • retina development in camera-type eye Source: MGI
  • sensory perception of light stimulus Source: UniProtKB
  • signal transduction Source: MGI
  • synaptic transmission Source: Ensembl
  • visual perception Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Sensory transduction, Vision

Enzyme and pathway databases

ReactomeiR-MMU-418594. G alpha (i) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 6
Short name:
mGluR6
Gene namesi
Name:Grm6
Synonyms:Gprc1f, Mglur6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1351343. Grm6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 579556ExtracellularSequence analysisAdd
BLAST
Transmembranei580 – 60223Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini603 – 61614CytoplasmicSequence analysisAdd
BLAST
Transmembranei617 – 63721Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini638 – 64811ExtracellularSequence analysisAdd
BLAST
Transmembranei649 – 66719Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini668 – 69124CytoplasmicSequence analysisAdd
BLAST
Transmembranei692 – 71221Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini713 – 74230ExtracellularSequence analysisAdd
BLAST
Transmembranei743 – 76422Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini765 – 77713CytoplasmicSequence analysisAdd
BLAST
Transmembranei778 – 80023Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini801 – 81313ExtracellularSequence analysisAdd
BLAST
Transmembranei814 – 83926Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini840 – 87132CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • dendrite Source: UniProtKB-SubCell
  • endoplasmic reticulum membrane Source: UniProtKB
  • Golgi membrane Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • new growing cell tip Source: MGI
  • postsynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Retinal cells from mutant mice display a subtly altered response to cycles of light and darkness, due to a failure of the ON bipolar cells in the retina to become depolarized in response to light. As a consequence, mutant mice display little or no pupillary contraction in adaptation to low light intensity. Besides, they exhibit strongly impaired responses to moving stimuli, and fail to produce a response when the visual constrast is low. Besides, rod bipolar cells from mutant mice lack TRPM1 channel activity.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 871848Metabotropic glutamate receptor 6PRO_0000012935Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 93By similarity
Disulfide bondi238 ↔ 530By similarity
Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence analysis
Disulfide bondi361 ↔ 377By similarity
Disulfide bondi417 ↔ 424By similarity
Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence analysis
Glycosylationi473 – 4731N-linked (GlcNAc...)Sequence analysis
Disulfide bondi512 ↔ 531By similarity
Disulfide bondi516 ↔ 534By similarity
Disulfide bondi537 ↔ 549By similarity
Disulfide bondi552 ↔ 565By similarity
Glycosylationi561 – 5611N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5NCH9.
PRIDEiQ5NCH9.

PTM databases

PhosphoSiteiQ5NCH9.

Expressioni

Tissue specificityi

Detected in the outer plexiform layer in retina (at protein level).2 Publications

Gene expression databases

BgeeiQ5NCH9.
CleanExiMM_GRM6.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi223810. 1 interaction.
STRINGi10090.ENSMUSP00000000631.

Structurei

3D structure databases

ProteinModelPortaliQ5NCH9.
SMRiQ5NCH9. Positions 26-845.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 1713Glutamate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiQ5NCH9.
KOiK04608.
OMAiYAIKARG.
OrthoDBiEOG7Z0JXG.
PhylomeDBiQ5NCH9.
TreeFamiTF313240.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000112. GPCR_3_mtglu_rcpt_6.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01056. MTABOTROPC6R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5NCH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRLRVLLLW LAWWLSQAGI AHGAGSVRLA GGLTLGGLFP VHARGAAGRA
60 70 80 90 100
CGTLKKEQGV HRLEAMLYAL DRINADPELL PGVRLGARLL DTCSRDTYAL
110 120 130 140 150
EQALSFVQAL IRGRGDGEEA SVRCPGGVPP LRAAPPERVV AVVGASASSV
160 170 180 190 200
SIMVANVLRL FAIPQISYAS TAPELSDSTR YDFFSRVVPP DSYQAQAMVD
210 220 230 240 250
IVRALGWNYV STLASEGNYG ESGVEAFVQI SREAGGVCIA QSIKIPREPK
260 270 280 290 300
PGEFHKVIRR LMETPNARGI IIFANEDDIR RVLEATRQAN LTGHFLWVGS
310 320 330 340 350
DSWGSKISPI LNLEEEAVGA ITILPKRASI DGFDQYFMTR SLENNRRNIW
360 370 380 390 400
FAEFWEENFN CKLTSSGGQS DDSTRKCTGE ERIGQDSTYE QEGKVQFVID
410 420 430 440 450
AVYAIAHALH SMHQALCPGH TGLCPAMEPT DGRTLLHYIR AVRFNGSAGT
460 470 480 490 500
PVMFNENGDA PGRYDIFQYQ ATNGSASSGG YQAVGQWAEA LRLDMEALQW
510 520 530 540 550
SGDPHEVPPS QCSLPCGPGE RKKMVKGVPC CWHCEACDGY RFQVDEFTCE
560 570 580 590 600
ACPGHMRPTP NHTGCRPTPV VRLTWSSPWA ALPLLLAVLG IMATTTIIAT
610 620 630 640 650
FMRHNDTPIV RASGRELSYV LLTGIFLIYA ITFLMVAEPC AAVCASRRLL
660 670 680 690 700
LGLGTTLSYS ALLTKTNRIY RIFEQGKRSV TPPPFISPTS QLVITFGLTS
710 720 730 740 750
LQVVGVIAWL GAQPPHSVID YEEQRTVDPE QARGVLKCDM SDLSLIGCLG
760 770 780 790 800
YSLLLMVTCT VYAIKARGVP ETFNEAKPIG FTMYTTCIIW LAFVPIFFGT
810 820 830 840 850
AQSAEKIYIQ TTTLTVSLSL SASVSLGMLY VPKTYVILFH PEQNVQKRKR
860 870
SLKKTSTMAA PPKSENSEDA K
Length:871
Mass (Da):95,193
Last modified:February 1, 2005 - v1
Checksum:iFB07056ABA7B259D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL627215 Genomic DNA. Translation: CAI35295.1.
CCDSiCCDS48785.1.
RefSeqiNP_775548.2. NM_173372.2.
XP_006532024.1. XM_006531961.2.
UniGeneiMm.134265.

Genome annotation databases

EnsembliENSMUST00000000631; ENSMUSP00000000631; ENSMUSG00000000617.
ENSMUST00000171427; ENSMUSP00000130728; ENSMUSG00000000617.
GeneIDi108072.
KEGGimmu:108072.
UCSCiuc007isu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL627215 Genomic DNA. Translation: CAI35295.1.
CCDSiCCDS48785.1.
RefSeqiNP_775548.2. NM_173372.2.
XP_006532024.1. XM_006531961.2.
UniGeneiMm.134265.

3D structure databases

ProteinModelPortaliQ5NCH9.
SMRiQ5NCH9. Positions 26-845.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223810. 1 interaction.
STRINGi10090.ENSMUSP00000000631.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ5NCH9.

Proteomic databases

PaxDbiQ5NCH9.
PRIDEiQ5NCH9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000631; ENSMUSP00000000631; ENSMUSG00000000617.
ENSMUST00000171427; ENSMUSP00000130728; ENSMUSG00000000617.
GeneIDi108072.
KEGGimmu:108072.
UCSCiuc007isu.2. mouse.

Organism-specific databases

CTDi2916.
MGIiMGI:1351343. Grm6.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiQ5NCH9.
KOiK04608.
OMAiYAIKARG.
OrthoDBiEOG7Z0JXG.
PhylomeDBiQ5NCH9.
TreeFamiTF313240.

Enzyme and pathway databases

ReactomeiR-MMU-418594. G alpha (i) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

ChiTaRSiGrm6. mouse.
NextBioi359992.
PROiQ5NCH9.
SOURCEiSearch...

Gene expression databases

BgeeiQ5NCH9.
CleanExiMM_GRM6.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000112. GPCR_3_mtglu_rcpt_6.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01056. MTABOTROPC6R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene."
    Masu M., Iwakabe H., Tagawa Y., Miyoshi T., Yamashita M., Fukuda Y., Sasaki H., Hiroi K., Nakamura Y., Shigemoto R., Takada M., Nakamura K., Nakao K., Katsuki M., Nakanishi S.
    Cell 80:757-765(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  3. "Impairment of pupillary responses and optokinetic nystagmus in the mGluR6-deficient mouse."
    Iwakabe H., Katsuura G., Ishibashi C., Nakanishi S.
    Neuropharmacology 36:135-143(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  4. "Gbeta5-RGS complexes co-localize with mGluR6 in retinal ON-bipolar cells."
    Morgans C.W., Wensel T.G., Brown R.L., Perez-Leon J.A., Bearnot B., Duvoisin R.M.
    Eur. J. Neurosci. 26:2899-2905(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Effects of presynaptic mutations on a postsynaptic Cacna1s calcium channel colocalized with mGluR6 at mouse photoreceptor ribbon synapses."
    Specht D., Wu S.B., Turner P., Dearden P., Koentgen F., Wolfrum U., Maw M., Brandstatter J.H., tom Dieck S.
    Invest. Ophthalmol. Vis. Sci. 50:505-515(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "mGluR6 deletion renders the TRPM1 channel in retina inactive."
    Xu Y., Dhingra A., Fina M.E., Koike C., Furukawa T., Vardi N.
    J. Neurophysiol. 107:948-957(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiGRM6_MOUSE
AccessioniPrimary (citable) accession number: Q5NCH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: February 1, 2005
Last modified: May 11, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.