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Protein

XIAP-associated factor 1

Gene

Xaf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri22 – 8059TRAF-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
XIAP-associated factor 1
Alternative name(s):
BIRC4-binding protein
Gene namesi
Name:Xaf1
Synonyms:Birc4bp, Xiapaf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:3772572. Xaf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273XIAP-associated factor 1PRO_0000329029Add
BLAST

Proteomic databases

EPDiQ5NBU8.
MaxQBiQ5NBU8.
PaxDbiQ5NBU8.
PRIDEiQ5NBU8.

PTM databases

PhosphoSiteiQ5NBU8.

Expressioni

Gene expression databases

BgeeiQ5NBU8.
ExpressionAtlasiQ5NBU8. baseline and differential.
GenevisibleiQ5NBU8. MM.

Interactioni

Subunit structurei

Interacts with BIRC1, BIRC2, BIRC3, BIRC4, BIRC7 and BIRC8. Part of an complex consisting of BIRC4, XAF1 and BIRC5; the complex formation requires IFN-beta stimulation. Interacts with RNF114, the interaction increases XAF1 stability and proapoptotic effects, and may regulate IFN signaling (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000123011.

Structurei

3D structure databases

ProteinModelPortaliQ5NBU8.
SMRiQ5NBU8. Positions 59-118, 228-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 TRAF-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri22 – 8059TRAF-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IK96. Eukaryota.
ENOG4110HW5. LUCA.
GeneTreeiENSGT00530000063869.
HOGENOMiHOG000154869.
HOVERGENiHBG108681.
InParanoidiQ5NBU8.
OMAiPRENMEE.
OrthoDBiEOG73806B.
PhylomeDBiQ5NBU8.
TreeFamiTF331416.

Family and domain databases

InterProiIPR031220. XAF1.
[Graphical view]
PANTHERiPTHR16295:SF17. PTHR16295:SF17. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5NBU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEADFQVCRN CKRNVASLHF MLHEAHCLRF IVLCPECEEP IPESKMKEHM
60 70 80 90 100
EVVHQQTKES QQHPAKCKFC ELAVQLSNLD VHESHCGSRT EHCPHCNQPI
110 120 130 140 150
TLQVLSQHKA MCLSAKGRPE EGKRIVSSPG RKTRCDLCKQ MIPENTYASH
160 170 180 190 200
MKQCSAPNTV TRIRDESIIV IPSTLAFMDS GNRRSTVSKD VRPKTKNRNS
210 220 230 240 250
STKRETKKQN GTVALPLKSG LQQRADLPTG DETAYDTLQN CCQCRILLPL
260 270
PILNEHQEKC QRLAHQKKLQ WGW
Length:273
Mass (Da):31,117
Last modified:April 8, 2008 - v3
Checksum:i89D504585DACEFCA
GO
Isoform 2 (identifier: Q5NBU8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     57-151: Missing.

Note: Gene prediction based on EST data.
Show »
Length:178
Mass (Da):20,465
Checksum:i0BE1122DADB70663
GO

Sequence cautioni

The sequence BAE24328.1 differs from that shown.Probable cloning artifact.Curated
The sequence CAI36034.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei57 – 15195Missing in isoform 2. CuratedVSP_039715Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL929071 Genomic DNA. Translation: CAI36033.2.
AL929071 Genomic DNA. Translation: CAI36034.1. Sequence problems.
AL929071 Genomic DNA. Translation: CAX15644.1.
AK140315 mRNA. Translation: BAE24328.1. Sequence problems.
CCDSiCCDS24984.2. [Q5NBU8-1]
CCDS70236.1. [Q5NBU8-3]
RefSeqiNP_001032802.2. NM_001037713.4. [Q5NBU8-1]
NP_001278082.1. NM_001291153.1. [Q5NBU8-3]
UniGeneiMm.291131.

Genome annotation databases

EnsembliENSMUST00000140842; ENSMUSP00000121472; ENSMUSG00000040483. [Q5NBU8-3]
ENSMUST00000146233; ENSMUSP00000123011; ENSMUSG00000040483. [Q5NBU8-1]
GeneIDi327959.
KEGGimmu:327959.
UCSCiuc007jyr.2. mouse. [Q5NBU8-1]
uc056ymf.1. mouse. [Q5NBU8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL929071 Genomic DNA. Translation: CAI36033.2.
AL929071 Genomic DNA. Translation: CAI36034.1. Sequence problems.
AL929071 Genomic DNA. Translation: CAX15644.1.
AK140315 mRNA. Translation: BAE24328.1. Sequence problems.
CCDSiCCDS24984.2. [Q5NBU8-1]
CCDS70236.1. [Q5NBU8-3]
RefSeqiNP_001032802.2. NM_001037713.4. [Q5NBU8-1]
NP_001278082.1. NM_001291153.1. [Q5NBU8-3]
UniGeneiMm.291131.

3D structure databases

ProteinModelPortaliQ5NBU8.
SMRiQ5NBU8. Positions 59-118, 228-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000123011.

PTM databases

PhosphoSiteiQ5NBU8.

Proteomic databases

EPDiQ5NBU8.
MaxQBiQ5NBU8.
PaxDbiQ5NBU8.
PRIDEiQ5NBU8.

Protocols and materials databases

DNASUi327959.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000140842; ENSMUSP00000121472; ENSMUSG00000040483. [Q5NBU8-3]
ENSMUST00000146233; ENSMUSP00000123011; ENSMUSG00000040483. [Q5NBU8-1]
GeneIDi327959.
KEGGimmu:327959.
UCSCiuc007jyr.2. mouse. [Q5NBU8-1]
uc056ymf.1. mouse. [Q5NBU8-3]

Organism-specific databases

CTDi54739.
MGIiMGI:3772572. Xaf1.

Phylogenomic databases

eggNOGiENOG410IK96. Eukaryota.
ENOG4110HW5. LUCA.
GeneTreeiENSGT00530000063869.
HOGENOMiHOG000154869.
HOVERGENiHBG108681.
InParanoidiQ5NBU8.
OMAiPRENMEE.
OrthoDBiEOG73806B.
PhylomeDBiQ5NBU8.
TreeFamiTF331416.

Miscellaneous databases

NextBioi398041.
PROiQ5NBU8.
SOURCEiSearch...

Gene expression databases

BgeeiQ5NBU8.
ExpressionAtlasiQ5NBU8. baseline and differential.
GenevisibleiQ5NBU8. MM.

Family and domain databases

InterProiIPR031220. XAF1.
[Graphical view]
PANTHERiPTHR16295:SF17. PTHR16295:SF17. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-205 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Adipose tissue.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiXAF1_MOUSE
AccessioniPrimary (citable) accession number: Q5NBU8
Secondary accession number(s): B7ZD14
, Q3USK3, Q5NBU6, Q5NBU7, Q5NBU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: March 16, 2016
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.