ID   GLU2B_ORYSJ             Reviewed;         614 AA.
AC   Q5NBP9; A0A0P0V0Y0; B9EV73;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   24-JAN-2024, entry version 129.
DE   RecName: Full=Glucosidase 2 subunit beta;
DE   AltName: Full=Glucosidase II subunit beta;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os01g0276800; ORFNames=OsJ_01291, P0038F12.26;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Regulatory subunit of glucosidase II. May be required for
CC       defense response elicited by pathogen-associated molecular patterns
CC       (PAMPs) (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Glycan metabolism; N-glycan metabolism.
CC   -!- SUBUNIT: Heterodimer of a catalytic alpha subunit and a beta subunit.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000255|PROSITE-
CC       ProRule:PRU10138}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP000836; BAD81117.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF04646.1; -; Genomic_DNA.
DR   EMBL; AP014957; BAS71556.1; -; Genomic_DNA.
DR   EMBL; CM000138; EEE54323.1; -; Genomic_DNA.
DR   EMBL; AK102926; BAG95785.1; -; mRNA.
DR   RefSeq; XP_015621560.1; XM_015766074.1.
DR   RefSeq; XP_015621562.1; XM_015766076.1.
DR   AlphaFoldDB; Q5NBP9; -.
DR   SMR; Q5NBP9; -.
DR   STRING; 39947.Q5NBP9; -.
DR   PaxDb; 39947-Q5NBP9; -.
DR   EnsemblPlants; Os01t0276800-01; Os01t0276800-01; Os01g0276800.
DR   GeneID; 4324264; -.
DR   Gramene; Os01t0276800-01; Os01t0276800-01; Os01g0276800.
DR   KEGG; osa:4324264; -.
DR   eggNOG; KOG2397; Eukaryota.
DR   HOGENOM; CLU_016834_3_0_1; -.
DR   InParanoid; Q5NBP9; -.
DR   OMA; YENGQHC; -.
DR   OrthoDB; 103990at2759; -.
DR   BRENDA; 3.2.1.207; 4460.
DR   UniPathway; UPA00957; -.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000007752; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   ExpressionAtlas; Q5NBP9; baseline and differential.
DR   GO; GO:0017177; C:glucosidase II complex; IBA:GO_Central.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0006491; P:N-glycan processing; IBA:GO_Central.
DR   Gene3D; 2.70.130.10; Mannose-6-phosphate receptor binding domain; 1.
DR   InterPro; IPR039794; Gtb1-like.
DR   InterPro; IPR009011; Man6P_isomerase_rcpt-bd_dom_sf.
DR   InterPro; IPR044865; MRH_dom.
DR   InterPro; IPR036607; PRKCSH.
DR   InterPro; IPR028146; PRKCSH_N.
DR   PANTHER; PTHR12630:SF1; GLUCOSIDASE 2 SUBUNIT BETA; 1.
DR   PANTHER; PTHR12630; N-LINKED OLIGOSACCHARIDE PROCESSING; 1.
DR   Pfam; PF12999; PRKCSH-like; 1.
DR   Pfam; PF13015; PRKCSH_1; 1.
DR   SUPFAM; SSF50911; Mannose 6-phosphate receptor domain; 1.
DR   PROSITE; PS00014; ER_TARGET; 1.
DR   PROSITE; PS51914; MRH; 1.
DR   Genevisible; Q5NBP9; OS.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Endoplasmic reticulum; Glycoprotein; Plant defense;
KW   Reference proteome; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..614
FT                   /note="Glucosidase 2 subunit beta"
FT                   /id="PRO_0000425977"
FT   DOMAIN          497..592
FT                   /note="MRH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01262"
FT   REGION          194..396
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        194..276
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        305..355
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        368..386
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        115
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        499..512
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01262"
FT   DISULFID        549..578
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01262"
FT   DISULFID        563..590
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01262"
FT   CONFLICT        144
FT                   /note="V -> F (in Ref. 5; EEE54323)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   614 AA;  69316 MW;  2105F4D8F290DCA5 CRC64;
     MGLHAILLLL LLRISASAAA SRPPLDTLGI PPQDEAYFRG GVIRCRDGSG RFARDKLNDD
     FCDCPDGTDE PGTSACPEGK FYCQNAGHSP ITIFSSRVND GICDCCDGSD EYDSNVTCKN
     TCWEAGKAAR DKLKKKVATY KSGVVIRNQE IQKAKVAFAK DEAELAKLKG EEKILQGLVD
     KLTEQKKLIE KAEEEERLRK EKEEKRMKEE AEKQAADEKK ASDASQEVDS QENHETVQED
     ESKVAEHHDG HATSHDNHTP ESESSVEQHD PESQDDISIK AAPADESPPE ETSAAPTKEQ
     ESTPADSEGL SREELGRLVA SRWTGEKVDE VSKDDKNEHE AEHDMPEHSE ETHEDESDVP
     ESAEDSYAGY HSEVEDDRHK YDDEDFSHES DDEYVDDHDE HVASYKSDDD QKGDDHSDFT
     ASGQASWLDK IQQTVQNVLR TFNFFKTPVD LSEASRVRKE YDDASSKLSK IQSRISTLTD
     KLKHDFGKEK EFYYFYDQCF ESKEGKYVYK VCPFKKASQV EGHSTTSLGR WDKFEESYRV
     MQFSNGDRCW NGPDRSLKVR LRCGLNNELN GVDEPSRCEY VAVLSTPALC DEQKLKELEQ
     KLKASSNQRD HDEL
//