Q5NAT8 (GUN1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 1
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 1
OsCel9B
OsGLU7

Gene names

Name:	GLU7
Ordered Locus Names:	Os01g0219600, LOC_Os01g12030
ORF Names:	OsJ_000887, P0483F08.8, P0489G09.23

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in roots, leaf sheaths and flowers. Ref.6
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 640	616	Endoglucanase 1		PRO_0000249278
Sites
	Active site	433	1	By similarity
	Active site	485	1	By similarity
	Active site	494	1	By similarity
Amino acid modifications
	Glycosylation	528	1	N-linked (GlcNAc...) Potential
	Glycosylation	548	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	605	1	L → F in AK111165. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q5NAT8 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 3C9224D956FB441B	FASTA	640	67,904
	10 20 30 40 50 60 MARRGGAAAS SSMANLLGVA LVLAATAQTS ARGGGGGGRH DYRMALSKSI LYFEAQRSGV 70 80 90 100 110 120 LPGNQRIAWR ANSGLADGKA NGVDLVGGYY DAGDNVKFGF PMAFTVTMMA WSVLEYGKQM 130 140 150 160 170 180 AAAGELGHAM DAVRWGADYF VKAHPAPNVL YGEVGDGDSD HVCWQRPEDM TTSRQAYRLD 190 200 210 220 230 240 PQHPGSDLAG ETATALAAAS LVFRSSNPGY ANQLLQHSKQ LFDFADKYRG KYDDSMPVVK 250 260 270 280 290 300 KFYGSFSGYG DELLWASAWL YQATDNRRYL DYLANNGDAL GGTGWATNEF GWDVKYPGVQ 310 320 330 340 350 360 VLAAKFLLQG KAGPHAAVLR RYQRNADVFA CSCLGKGGGG GNVGRTPGGL MYHQGWNNLQ 370 380 390 400 410 420 FVTGASFLLA VYADHLAAAG RGQAVVRCQA GPAARASELV ALAKSQVDYI LGSNPRGISY 430 440 450 460 470 480 MVGYGARYPR RAHHRGASIV SIRANPSFVS CKDGYASWFG RAGSNPNLLD GAVVGGPDGR 490 500 510 520 530 540 DGFADERNNY QQTEVATYNN APLMGVLARL AGGGRGGLAE AAIKRPDNQT LLPPLAAAAS 550 560 570 580 590 600 PVEITQLNAT ASWKKDGRTY RRYAATVSNR SPAGGKTVEE LHIGIGKPHG PVWGLEKAAR 610 620 630 640 YGYVLPSSLA AGESAAFAYV VRGRAAPPPA DVWVIGYKLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP002094 Genomic DNA. Translation: BAD81358.1. AP002745 Genomic DNA. Translation: BAD81424.1. AP008207 Genomic DNA. Translation: BAF04335.1. CM000138 Genomic DNA. Translation: EAZ11062.1. AK111165 mRNA. No translation available.
RefSeq	NP_001042421.1. NM_001048956.1.
UniGene	Os.80657.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q5NAT8.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os01g12030.1.
Protein family/group databases
CAZy	CBM49. Carbohydrate-Binding Module Family 49. GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	Q5NAT8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4324641.
KEGG	dosa:Os01t0219600-00. osa:4324641.
Organism-specific databases
Gramene	Q5NAT8.
Phylogenomic databases
eggNOG	NOG05134.
OMA	IVSIRAN.
ProtClustDB	PLN02171.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR019028. CBM_49. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF09478. CBM49. 1 hit. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_ORYSJ

Accession

Primary (citable) accession number: Q5NAT8
Secondary accession number(s): Q0JPJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	February 1, 2005
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program