Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5NAT8 (GUN1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 1

EC=3.2.1.4
Alternative name(s):
Endo-1,4-beta glucanase 1
OsCel9B
OsGLU7
Gene names
Name:GLU7
Ordered Locus Names:Os01g0219600, LOC_Os01g12030
ORF Names:OsJ_000887, P0483F08.8, P0489G09.23
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in roots, leaf sheaths and flowers. Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cell wall biogenesis/degradation
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

cellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 640616Endoglucanase 1
PRO_0000249278

Sites

Active site4331 By similarity
Active site4851 By similarity
Active site4941 By similarity

Amino acid modifications

Glycosylation5281N-linked (GlcNAc...) Potential
Glycosylation5481N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict6051L → F in AK111165. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q5NAT8 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 3C9224D956FB441B

FASTA64067,904
        10         20         30         40         50         60 
MARRGGAAAS SSMANLLGVA LVLAATAQTS ARGGGGGGRH DYRMALSKSI LYFEAQRSGV 

        70         80         90        100        110        120 
LPGNQRIAWR ANSGLADGKA NGVDLVGGYY DAGDNVKFGF PMAFTVTMMA WSVLEYGKQM 

       130        140        150        160        170        180 
AAAGELGHAM DAVRWGADYF VKAHPAPNVL YGEVGDGDSD HVCWQRPEDM TTSRQAYRLD 

       190        200        210        220        230        240 
PQHPGSDLAG ETATALAAAS LVFRSSNPGY ANQLLQHSKQ LFDFADKYRG KYDDSMPVVK 

       250        260        270        280        290        300 
KFYGSFSGYG DELLWASAWL YQATDNRRYL DYLANNGDAL GGTGWATNEF GWDVKYPGVQ 

       310        320        330        340        350        360 
VLAAKFLLQG KAGPHAAVLR RYQRNADVFA CSCLGKGGGG GNVGRTPGGL MYHQGWNNLQ 

       370        380        390        400        410        420 
FVTGASFLLA VYADHLAAAG RGQAVVRCQA GPAARASELV ALAKSQVDYI LGSNPRGISY 

       430        440        450        460        470        480 
MVGYGARYPR RAHHRGASIV SIRANPSFVS CKDGYASWFG RAGSNPNLLD GAVVGGPDGR 

       490        500        510        520        530        540 
DGFADERNNY QQTEVATYNN APLMGVLARL AGGGRGGLAE AAIKRPDNQT LLPPLAAAAS 

       550        560        570        580        590        600 
PVEITQLNAT ASWKKDGRTY RRYAATVSNR SPAGGKTVEE LHIGIGKPHG PVWGLEKAAR 

       610        620        630        640 
YGYVLPSSLA AGESAAFAYV VRGRAAPPPA DVWVIGYKLV 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[6]"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation."
Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y.
Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP002094 Genomic DNA. Translation: BAD81358.1.
AP002745 Genomic DNA. Translation: BAD81424.1.
AP008207 Genomic DNA. Translation: BAF04335.1.
CM000138 Genomic DNA. Translation: EAZ11062.1.
AK111165 mRNA. No translation available.
RefSeqNP_001042421.1. NM_001048956.1.
UniGeneOs.80657.

3D structure databases

ProteinModelPortalQ5NAT8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os01g12030.1.

Protein family/group databases

CAZyCBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PRIDEQ5NAT8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS01T0219600-00; OS01T0219600-00; OS01G0219600.
GeneID4324641.
KEGGosa:4324641.

Organism-specific databases

GrameneQ5NAT8.

Phylogenomic databases

eggNOGNOG05134.
OMAIVSIRAN.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN1_ORYSJ
AccessionPrimary (citable) accession number: Q5NAT8
Secondary accession number(s): Q0JPJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries