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Q5NAT0 (GUN2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 2

EC=3.2.1.4
Alternative name(s):
Endo-1,4-beta glucanase 2
OsCel9A
OsGLU5
Gene names
Name:GLU5
Ordered Locus Names:Os01g0220100, LOC_Os01g12070
ORF Names:OsJ_000889, P0483F08.13, P0489G09.28
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes 1,4-beta-glycosyl linkages of 1,4-beta-glucans and 1,3-1,4-beta-glucans. Possesses broad substrate specificity for hemicelluloses of type II cell walls. Substrate preference is carboxymethyl-cellulose > 1,3-1,4-beta-glucan > lichenan > arabinoxylan > phospho-swollen cellulose > xylan > glucomannan. May participate in lateral root development. Ref.1 Ref.2

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in roots and flowers. Ref.8

Developmental stage

Expressed in lateral root primordia during auxin-induced lateral root development. Ref.2

Induction

By auxin in roots (at protein level). Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Biophysicochemical properties

pH dependence:

Optimum pH is 5.3-5.6 with 1,3-1,4-beta-glucan as substrate (at 35 degrees Celsius). Ref.1

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Ref.1
Chain35 – 511477Endoglucanase 2
PRO_0000249279
Propeptide512 – 640129Removed in mature form
PRO_0000372490

Sites

Active site4281 By similarity
Active site4801 By similarity
Active site4891 By similarity

Amino acid modifications

Glycosylation5281N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict6161G → S in AK103304. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q5NAT0 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: D68E16BB1B3A35CA

FASTA64068,408
        10         20         30         40         50         60 
MARGGGAAGV SMAHHLGIAL VVLVFAAMAQ VARGGGGGHD YGMALSKSIL YFEAQRSGVL 

        70         80         90        100        110        120 
PGSQRIAWRA NSGLADGKAN GVDLVGGYYD AGDNVKFGLP MAFTVTMMAW SVIEYGEEMA 

       130        140        150        160        170        180 
AAGELGHAVE AIKWGTDYFA KAHPEPNVLY AEVGDGDSDH NCWQRPEDMT TSRQAYRLDP 

       190        200        210        220        230        240 
QNPGSDLAGE TAAAMAAASL VFRSSNPGYA DQLLQHSKQL FDFADKYRGR YDNSITVARN 

       250        260        270        280        290        300 
YYGSFSGYGD ELLWASAWLY QASDDRRYLD YLANNADALG GTGWSINQFG WDVKYPGVQI 

       310        320        330        340        350        360 
LAAKFLLQGK AGEHAGVLQG YRRKADFFAC SCLGKDAADN VGRTPGGMLY HQRWNNIQFV 

       370        380        390        400        410        420 
TSASFLLAVY SDHLAGGAVR CSGGGGAVAG AAELLAFAKS QVDYILGSNP RGTSYMVGYG 

       430        440        450        460        470        480 
AVYPRQAHHR GSSIASIRAS PSFVSCREGY ASWYGRRGGN PNLLDGAVVG GPDEHDDFAD 

       490        500        510        520        530        540 
ERNNYEQTEA ATYNNAPLMG ILARLAAGHG ARARGRLGQS LQHGIAANHT SLPHGANHQH 

       550        560        570        580        590        600 
ASPVEIEQKA TASWEKDGRT YHRYAVTVSN RSPAGGKTVE ELHIGIGKLY GPVWGLEKAA 

       610        620        630        640 
RYGYVLPSWT PSLPAGESAA FVYVHAAPPA DVWVTGYKLV 

« Hide

References

« Hide 'large scale' references
[1]"A rice family 9 glycoside hydrolase isozyme with broad substrate specificity for hemicelluloses in type II cell walls."
Yoshida K., Komae K.
Plant Cell Physiol. 47:1541-1554(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-64; 48-167; 219-274 AND 295-335, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: cv. Sasanishiki.
Tissue: Root.
[2]"Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A, expressed in auxin-induced lateral root primordia, is post-translationally truncated."
Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H., Nishitani K., Komae K.
Plant Cell Physiol. 47:1555-1571(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
Strain: cv. Sasanishiki.
Tissue: Root.
[3]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[6]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[7]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[8]"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation."
Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y.
Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB038510 mRNA. Translation: BAF37260.1.
AP002094 Genomic DNA. Translation: BAD81360.1.
AP002745 Genomic DNA. Translation: BAD81426.1.
AP008207 Genomic DNA. Translation: BAF04337.1.
CM000138 Genomic DNA. Translation: EAZ11064.1.
AK103304 mRNA. No translation available.
RefSeqNP_001042423.1. NM_001048958.1.
UniGeneOs.32667.

3D structure databases

ProteinModelPortalQ5NAT0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os01g12070.1.

Protein family/group databases

CAZyCBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS01T0220100-01; OS01T0220100-01; OS01G0220100.
GeneID4324643.
KEGGosa:4324643.

Organism-specific databases

GrameneQ5NAT0.

Phylogenomic databases

eggNOGNOG05134.
HOGENOMHOG000021033.
OMAAVEAIKW.
ProtClustDBPLN02171.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTSM01063. CBM49. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN2_ORYSJ
AccessionPrimary (citable) accession number: Q5NAT0
Secondary accession number(s): A0P889, Q0JPJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries