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Q5NAT0

- GUN2_ORYSJ

UniProt

Q5NAT0 - GUN2_ORYSJ

Protein

Endoglucanase 2

Gene

GLU5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Hydrolyzes 1,4-beta-glycosyl linkages of 1,4-beta-glucans and 1,3-1,4-beta-glucans. Possesses broad substrate specificity for hemicelluloses of type II cell walls. Substrate preference is carboxymethyl-cellulose > 1,3-1,4-beta-glucan > lichenan > arabinoxylan > phospho-swollen cellulose > xylan > glucomannan. May participate in lateral root development.2 Publications

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    pH dependencei

    Optimum pH is 5.3-5.6 with 1,3-1,4-beta-glucan as substrate (at 35 degrees Celsius).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei428 – 4281PROSITE-ProRule annotation
    Active sitei480 – 4801PROSITE-ProRule annotation
    Active sitei489 – 4891PROSITE-ProRule annotation

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM49. Carbohydrate-Binding Module Family 49.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 2 (EC:3.2.1.4)
    Alternative name(s):
    Endo-1,4-beta glucanase 2
    OsCel9A
    OsGLU5
    Gene namesi
    Name:GLU5
    Ordered Locus Names:Os01g0220100, LOC_Os01g12070
    ORF Names:OsJ_000889, P0483F08.13, P0489G09.28
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 1

    Organism-specific databases

    GrameneiQ5NAT0.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 34341 PublicationAdd
    BLAST
    Chaini35 – 511477Endoglucanase 2PRO_0000249279Add
    BLAST
    Propeptidei512 – 640129Removed in mature formPRO_0000372490Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed in roots and flowers.1 Publication

    Developmental stagei

    Expressed in lateral root primordia during auxin-induced lateral root development.1 Publication

    Inductioni

    By auxin in roots (at protein level).1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os01g12070.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5NAT0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG05134.
    HOGENOMiHOG000021033.
    OMAiDRRYLDY.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR019028. CBM_49.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF09478. CBM49. 1 hit.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SMARTiSM01063. CBM49. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5NAT0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARGGGAAGV SMAHHLGIAL VVLVFAAMAQ VARGGGGGHD YGMALSKSIL    50
    YFEAQRSGVL PGSQRIAWRA NSGLADGKAN GVDLVGGYYD AGDNVKFGLP 100
    MAFTVTMMAW SVIEYGEEMA AAGELGHAVE AIKWGTDYFA KAHPEPNVLY 150
    AEVGDGDSDH NCWQRPEDMT TSRQAYRLDP QNPGSDLAGE TAAAMAAASL 200
    VFRSSNPGYA DQLLQHSKQL FDFADKYRGR YDNSITVARN YYGSFSGYGD 250
    ELLWASAWLY QASDDRRYLD YLANNADALG GTGWSINQFG WDVKYPGVQI 300
    LAAKFLLQGK AGEHAGVLQG YRRKADFFAC SCLGKDAADN VGRTPGGMLY 350
    HQRWNNIQFV TSASFLLAVY SDHLAGGAVR CSGGGGAVAG AAELLAFAKS 400
    QVDYILGSNP RGTSYMVGYG AVYPRQAHHR GSSIASIRAS PSFVSCREGY 450
    ASWYGRRGGN PNLLDGAVVG GPDEHDDFAD ERNNYEQTEA ATYNNAPLMG 500
    ILARLAAGHG ARARGRLGQS LQHGIAANHT SLPHGANHQH ASPVEIEQKA 550
    TASWEKDGRT YHRYAVTVSN RSPAGGKTVE ELHIGIGKLY GPVWGLEKAA 600
    RYGYVLPSWT PSLPAGESAA FVYVHAAPPA DVWVTGYKLV 640
    Length:640
    Mass (Da):68,408
    Last modified:February 1, 2005 - v1
    Checksum:iD68E16BB1B3A35CA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti616 – 6161G → S in AK103304. (PubMed:12869764)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038510 mRNA. Translation: BAF37260.1.
    AP002094 Genomic DNA. Translation: BAD81360.1.
    AP002745 Genomic DNA. Translation: BAD81426.1.
    AP008207 Genomic DNA. Translation: BAF04337.1.
    CM000138 Genomic DNA. Translation: EAZ11064.1.
    AK103304 mRNA. No translation available.
    RefSeqiNP_001042423.1. NM_001048958.1.
    UniGeneiOs.32667.

    Genome annotation databases

    EnsemblPlantsiOS01T0220100-01; OS01T0220100-01; OS01G0220100.
    GeneIDi4324643.
    KEGGiosa:4324643.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038510 mRNA. Translation: BAF37260.1 .
    AP002094 Genomic DNA. Translation: BAD81360.1 .
    AP002745 Genomic DNA. Translation: BAD81426.1 .
    AP008207 Genomic DNA. Translation: BAF04337.1 .
    CM000138 Genomic DNA. Translation: EAZ11064.1 .
    AK103304 mRNA. No translation available.
    RefSeqi NP_001042423.1. NM_001048958.1.
    UniGenei Os.32667.

    3D structure databases

    ProteinModelPortali Q5NAT0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os01g12070.1.

    Protein family/group databases

    CAZyi CBM49. Carbohydrate-Binding Module Family 49.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS01T0220100-01 ; OS01T0220100-01 ; OS01G0220100 .
    GeneIDi 4324643.
    KEGGi osa:4324643.

    Organism-specific databases

    Gramenei Q5NAT0.

    Phylogenomic databases

    eggNOGi NOG05134.
    HOGENOMi HOG000021033.
    OMAi DRRYLDY.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR019028. CBM_49.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF09478. CBM49. 1 hit.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SMARTi SM01063. CBM49. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A rice family 9 glycoside hydrolase isozyme with broad substrate specificity for hemicelluloses in type II cell walls."
      Yoshida K., Komae K.
      Plant Cell Physiol. 47:1541-1554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-64; 48-167; 219-274 AND 295-335, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: cv. Sasanishiki.
      Tissue: Root.
    2. "Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A, expressed in auxin-induced lateral root primordia, is post-translationally truncated."
      Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H., Nishitani K., Komae K.
      Plant Cell Physiol. 47:1555-1571(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
      Strain: cv. Sasanishiki.
      Tissue: Root.
    3. "The genome sequence and structure of rice chromosome 1."
      Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
      , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
      Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    5. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    6. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    7. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    8. "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation."
      Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y.
      Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGUN2_ORYSJ
    AccessioniPrimary (citable) accession number: Q5NAT0
    Secondary accession number(s): A0P889, Q0JPJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3