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Protein

Endoglucanase 2

Gene

GLU5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes 1,4-beta-glycosyl linkages of 1,4-beta-glucans and 1,3-1,4-beta-glucans. Possesses broad substrate specificity for hemicelluloses of type II cell walls. Substrate preference is carboxymethyl-cellulose > 1,3-1,4-beta-glucan > lichenan > arabinoxylan > phospho-swollen cellulose > xylan > glucomannan. May participate in lateral root development.2 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.3-5.6 with 1,3-1,4-beta-glucan as substrate (at 35 degrees Celsius).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei428PROSITE-ProRule annotation1
Active sitei480PROSITE-ProRule annotation1
Active sitei489PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM49 Carbohydrate-Binding Module Family 49
GH9 Glycoside Hydrolase Family 9

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 2 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 2
OsCel9A
OsGLU5
Gene namesi
Name:GLU5
Ordered Locus Names:Os01g0220100, LOC_Os01g12070
ORF Names:OsJ_000889, P0483F08.13, P0489G09.28
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 341 PublicationAdd BLAST34
ChainiPRO_000024927935 – 511Endoglucanase 2Add BLAST477
PropeptideiPRO_0000372490512 – 640Removed in mature formAdd BLAST129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi528N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5NAT0
PRIDEiQ5NAT0

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Developmental stagei

Expressed in lateral root primordia during auxin-induced lateral root development.1 Publication

Inductioni

By auxin in roots (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ5NAT0 differential
GenevisibleiQ5NAT0 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g12070.1

Structurei

3D structure databases

ProteinModelPortaliQ5NAT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IDXW Eukaryota
ENOG410XPC9 LUCA
HOGENOMiHOG000021033
InParanoidiQ5NAT0
OMAiYPRQAHH
OrthoDBiEOG093604NE

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR019028 CBM_49
IPR001701 Glyco_hydro_9
IPR033126 Glyco_hydro_9_Asp/Glu_AS
PfamiView protein in Pfam
PF09478 CBM49, 1 hit
PF00759 Glyco_hydro_9, 1 hit
SMARTiView protein in SMART
SM01063 CBM49, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit
PROSITEiView protein in PROSITE
PS00698 GLYCOSYL_HYDROL_F9_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5NAT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGGGAAGV SMAHHLGIAL VVLVFAAMAQ VARGGGGGHD YGMALSKSIL
60 70 80 90 100
YFEAQRSGVL PGSQRIAWRA NSGLADGKAN GVDLVGGYYD AGDNVKFGLP
110 120 130 140 150
MAFTVTMMAW SVIEYGEEMA AAGELGHAVE AIKWGTDYFA KAHPEPNVLY
160 170 180 190 200
AEVGDGDSDH NCWQRPEDMT TSRQAYRLDP QNPGSDLAGE TAAAMAAASL
210 220 230 240 250
VFRSSNPGYA DQLLQHSKQL FDFADKYRGR YDNSITVARN YYGSFSGYGD
260 270 280 290 300
ELLWASAWLY QASDDRRYLD YLANNADALG GTGWSINQFG WDVKYPGVQI
310 320 330 340 350
LAAKFLLQGK AGEHAGVLQG YRRKADFFAC SCLGKDAADN VGRTPGGMLY
360 370 380 390 400
HQRWNNIQFV TSASFLLAVY SDHLAGGAVR CSGGGGAVAG AAELLAFAKS
410 420 430 440 450
QVDYILGSNP RGTSYMVGYG AVYPRQAHHR GSSIASIRAS PSFVSCREGY
460 470 480 490 500
ASWYGRRGGN PNLLDGAVVG GPDEHDDFAD ERNNYEQTEA ATYNNAPLMG
510 520 530 540 550
ILARLAAGHG ARARGRLGQS LQHGIAANHT SLPHGANHQH ASPVEIEQKA
560 570 580 590 600
TASWEKDGRT YHRYAVTVSN RSPAGGKTVE ELHIGIGKLY GPVWGLEKAA
610 620 630 640
RYGYVLPSWT PSLPAGESAA FVYVHAAPPA DVWVTGYKLV
Length:640
Mass (Da):68,408
Last modified:February 1, 2005 - v1
Checksum:iD68E16BB1B3A35CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti616G → S in AK103304 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038510 mRNA Translation: BAF37260.1
AP002094 Genomic DNA Translation: BAD81360.1
AP002745 Genomic DNA Translation: BAD81426.1
AP008207 Genomic DNA Translation: BAF04337.1
AP014957 Genomic DNA Translation: BAS71064.1
CM000138 Genomic DNA Translation: EAZ11064.1
AK103304 mRNA No translation available.
RefSeqiXP_015621149.1, XM_015765663.1
UniGeneiOs.32667

Genome annotation databases

EnsemblPlantsiOs01t0220100-01; Os01t0220100-01; Os01g0220100
GeneIDi4324643
GrameneiOs01t0220100-01; Os01t0220100-01; Os01g0220100
KEGGiosa:4324643

Similar proteinsi

Entry informationi

Entry nameiGUN2_ORYSJ
AccessioniPrimary (citable) accession number: Q5NAT0
Secondary accession number(s): A0A0P0V072, A0P889, Q0JPJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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