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Q5NAT0

- GUN2_ORYSJ

UniProt

Q5NAT0 - GUN2_ORYSJ

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Protein

Endoglucanase 2

Gene

GLU5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes 1,4-beta-glycosyl linkages of 1,4-beta-glucans and 1,3-1,4-beta-glucans. Possesses broad substrate specificity for hemicelluloses of type II cell walls. Substrate preference is carboxymethyl-cellulose > 1,3-1,4-beta-glucan > lichenan > arabinoxylan > phospho-swollen cellulose > xylan > glucomannan. May participate in lateral root development.2 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.3-5.6 with 1,3-1,4-beta-glucan as substrate (at 35 degrees Celsius).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei428 – 4281PROSITE-ProRule annotation
Active sitei480 – 4801PROSITE-ProRule annotation
Active sitei489 – 4891PROSITE-ProRule annotation

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 2 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 2
OsCel9A
OsGLU5
Gene namesi
Name:GLU5
Ordered Locus Names:Os01g0220100, LOC_Os01g12070
ORF Names:OsJ_000889, P0483F08.13, P0489G09.28
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 1

Organism-specific databases

GrameneiQ5NAT0.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 34341 PublicationAdd
BLAST
Chaini35 – 511477Endoglucanase 2PRO_0000249279Add
BLAST
Propeptidei512 – 640129Removed in mature formPRO_0000372490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Developmental stagei

Expressed in lateral root primordia during auxin-induced lateral root development.1 Publication

Inductioni

By auxin in roots (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g12070.1.

Structurei

3D structure databases

ProteinModelPortaliQ5NAT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG05134.
HOGENOMiHOG000021033.
InParanoidiQ5NAT0.
OMAiDRRYLDY.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01063. CBM49. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5NAT0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARGGGAAGV SMAHHLGIAL VVLVFAAMAQ VARGGGGGHD YGMALSKSIL
60 70 80 90 100
YFEAQRSGVL PGSQRIAWRA NSGLADGKAN GVDLVGGYYD AGDNVKFGLP
110 120 130 140 150
MAFTVTMMAW SVIEYGEEMA AAGELGHAVE AIKWGTDYFA KAHPEPNVLY
160 170 180 190 200
AEVGDGDSDH NCWQRPEDMT TSRQAYRLDP QNPGSDLAGE TAAAMAAASL
210 220 230 240 250
VFRSSNPGYA DQLLQHSKQL FDFADKYRGR YDNSITVARN YYGSFSGYGD
260 270 280 290 300
ELLWASAWLY QASDDRRYLD YLANNADALG GTGWSINQFG WDVKYPGVQI
310 320 330 340 350
LAAKFLLQGK AGEHAGVLQG YRRKADFFAC SCLGKDAADN VGRTPGGMLY
360 370 380 390 400
HQRWNNIQFV TSASFLLAVY SDHLAGGAVR CSGGGGAVAG AAELLAFAKS
410 420 430 440 450
QVDYILGSNP RGTSYMVGYG AVYPRQAHHR GSSIASIRAS PSFVSCREGY
460 470 480 490 500
ASWYGRRGGN PNLLDGAVVG GPDEHDDFAD ERNNYEQTEA ATYNNAPLMG
510 520 530 540 550
ILARLAAGHG ARARGRLGQS LQHGIAANHT SLPHGANHQH ASPVEIEQKA
560 570 580 590 600
TASWEKDGRT YHRYAVTVSN RSPAGGKTVE ELHIGIGKLY GPVWGLEKAA
610 620 630 640
RYGYVLPSWT PSLPAGESAA FVYVHAAPPA DVWVTGYKLV
Length:640
Mass (Da):68,408
Last modified:February 1, 2005 - v1
Checksum:iD68E16BB1B3A35CA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti616 – 6161G → S in AK103304. (PubMed:12869764)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB038510 mRNA. Translation: BAF37260.1.
AP002094 Genomic DNA. Translation: BAD81360.1.
AP002745 Genomic DNA. Translation: BAD81426.1.
AP008207 Genomic DNA. Translation: BAF04337.1.
CM000138 Genomic DNA. Translation: EAZ11064.1.
AK103304 mRNA. No translation available.
RefSeqiNP_001042423.1. NM_001048958.1.
UniGeneiOs.32667.

Genome annotation databases

EnsemblPlantsiOS01T0220100-01; OS01T0220100-01; OS01G0220100.
GeneIDi4324643.
KEGGiosa:4324643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB038510 mRNA. Translation: BAF37260.1 .
AP002094 Genomic DNA. Translation: BAD81360.1 .
AP002745 Genomic DNA. Translation: BAD81426.1 .
AP008207 Genomic DNA. Translation: BAF04337.1 .
CM000138 Genomic DNA. Translation: EAZ11064.1 .
AK103304 mRNA. No translation available.
RefSeqi NP_001042423.1. NM_001048958.1.
UniGenei Os.32667.

3D structure databases

ProteinModelPortali Q5NAT0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os01g12070.1.

Protein family/group databases

CAZyi CBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS01T0220100-01 ; OS01T0220100-01 ; OS01G0220100 .
GeneIDi 4324643.
KEGGi osa:4324643.

Organism-specific databases

Gramenei Q5NAT0.

Phylogenomic databases

eggNOGi NOG05134.
HOGENOMi HOG000021033.
InParanoidi Q5NAT0.
OMAi DRRYLDY.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SMARTi SM01063. CBM49. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A rice family 9 glycoside hydrolase isozyme with broad substrate specificity for hemicelluloses in type II cell walls."
    Yoshida K., Komae K.
    Plant Cell Physiol. 47:1541-1554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-64; 48-167; 219-274 AND 295-335, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: cv. Sasanishiki.
    Tissue: Root.
  2. "Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A, expressed in auxin-induced lateral root primordia, is post-translationally truncated."
    Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H., Nishitani K., Komae K.
    Plant Cell Physiol. 47:1555-1571(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Strain: cv. Sasanishiki.
    Tissue: Root.
  3. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  6. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  7. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  8. "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation."
    Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y.
    Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGUN2_ORYSJ
AccessioniPrimary (citable) accession number: Q5NAT0
Secondary accession number(s): A0P889, Q0JPJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: February 1, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3