Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endoglucanase 2

Gene

GLU5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes 1,4-beta-glycosyl linkages of 1,4-beta-glucans and 1,3-1,4-beta-glucans. Possesses broad substrate specificity for hemicelluloses of type II cell walls. Substrate preference is carboxymethyl-cellulose > 1,3-1,4-beta-glucan > lichenan > arabinoxylan > phospho-swollen cellulose > xylan > glucomannan. May participate in lateral root development.2 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.3-5.6 with 1,3-1,4-beta-glucan as substrate (at 35 degrees Celsius).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei428PROSITE-ProRule annotation1
Active sitei480PROSITE-ProRule annotation1
Active sitei489PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 2 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 2
OsCel9A
OsGLU5
Gene namesi
Name:GLU5
Ordered Locus Names:Os01g0220100, LOC_Os01g12070
ORF Names:OsJ_000889, P0483F08.13, P0489G09.28
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 341 PublicationAdd BLAST34
ChainiPRO_000024927935 – 511Endoglucanase 2Add BLAST477
PropeptideiPRO_0000372490512 – 640Removed in mature formAdd BLAST129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi528N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5NAT0.

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Developmental stagei

Expressed in lateral root primordia during auxin-induced lateral root development.1 Publication

Inductioni

By auxin in roots (at protein level).1 Publication

Gene expression databases

GenevisibleiQ5NAT0. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g12070.1.

Structurei

3D structure databases

ProteinModelPortaliQ5NAT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IDXW. Eukaryota.
ENOG410XPC9. LUCA.
HOGENOMiHOG000021033.
InParanoidiQ5NAT0.
OMAiFVYVHAA.
OrthoDBiEOG093604NE.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
[Graphical view]
PfamiPF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01063. CBM49. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5NAT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGGGAAGV SMAHHLGIAL VVLVFAAMAQ VARGGGGGHD YGMALSKSIL
60 70 80 90 100
YFEAQRSGVL PGSQRIAWRA NSGLADGKAN GVDLVGGYYD AGDNVKFGLP
110 120 130 140 150
MAFTVTMMAW SVIEYGEEMA AAGELGHAVE AIKWGTDYFA KAHPEPNVLY
160 170 180 190 200
AEVGDGDSDH NCWQRPEDMT TSRQAYRLDP QNPGSDLAGE TAAAMAAASL
210 220 230 240 250
VFRSSNPGYA DQLLQHSKQL FDFADKYRGR YDNSITVARN YYGSFSGYGD
260 270 280 290 300
ELLWASAWLY QASDDRRYLD YLANNADALG GTGWSINQFG WDVKYPGVQI
310 320 330 340 350
LAAKFLLQGK AGEHAGVLQG YRRKADFFAC SCLGKDAADN VGRTPGGMLY
360 370 380 390 400
HQRWNNIQFV TSASFLLAVY SDHLAGGAVR CSGGGGAVAG AAELLAFAKS
410 420 430 440 450
QVDYILGSNP RGTSYMVGYG AVYPRQAHHR GSSIASIRAS PSFVSCREGY
460 470 480 490 500
ASWYGRRGGN PNLLDGAVVG GPDEHDDFAD ERNNYEQTEA ATYNNAPLMG
510 520 530 540 550
ILARLAAGHG ARARGRLGQS LQHGIAANHT SLPHGANHQH ASPVEIEQKA
560 570 580 590 600
TASWEKDGRT YHRYAVTVSN RSPAGGKTVE ELHIGIGKLY GPVWGLEKAA
610 620 630 640
RYGYVLPSWT PSLPAGESAA FVYVHAAPPA DVWVTGYKLV
Length:640
Mass (Da):68,408
Last modified:February 1, 2005 - v1
Checksum:iD68E16BB1B3A35CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti616G → S in AK103304 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038510 mRNA. Translation: BAF37260.1.
AP002094 Genomic DNA. Translation: BAD81360.1.
AP002745 Genomic DNA. Translation: BAD81426.1.
AP008207 Genomic DNA. Translation: BAF04337.1.
AP014957 Genomic DNA. Translation: BAS71064.1.
CM000138 Genomic DNA. Translation: EAZ11064.1.
AK103304 mRNA. No translation available.
RefSeqiXP_015621149.1. XM_015765663.1.
UniGeneiOs.32667.

Genome annotation databases

EnsemblPlantsiOS01T0220100-01; OS01T0220100-01; OS01G0220100.
GeneIDi4324643.
GrameneiOS01T0220100-01; OS01T0220100-01; OS01G0220100.
KEGGiosa:4324643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038510 mRNA. Translation: BAF37260.1.
AP002094 Genomic DNA. Translation: BAD81360.1.
AP002745 Genomic DNA. Translation: BAD81426.1.
AP008207 Genomic DNA. Translation: BAF04337.1.
AP014957 Genomic DNA. Translation: BAS71064.1.
CM000138 Genomic DNA. Translation: EAZ11064.1.
AK103304 mRNA. No translation available.
RefSeqiXP_015621149.1. XM_015765663.1.
UniGeneiOs.32667.

3D structure databases

ProteinModelPortaliQ5NAT0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os01g12070.1.

Protein family/group databases

CAZyiCBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbiQ5NAT0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS01T0220100-01; OS01T0220100-01; OS01G0220100.
GeneIDi4324643.
GrameneiOS01T0220100-01; OS01T0220100-01; OS01G0220100.
KEGGiosa:4324643.

Phylogenomic databases

eggNOGiENOG410IDXW. Eukaryota.
ENOG410XPC9. LUCA.
HOGENOMiHOG000021033.
InParanoidiQ5NAT0.
OMAiFVYVHAA.
OrthoDBiEOG093604NE.

Gene expression databases

GenevisibleiQ5NAT0. OS.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
[Graphical view]
PfamiPF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01063. CBM49. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUN2_ORYSJ
AccessioniPrimary (citable) accession number: Q5NAT0
Secondary accession number(s): A0A0P0V072, A0P889, Q0JPJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: February 1, 2005
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.