Reviewed, UniProtKB/Swiss-Prot Q5N9X2 (LAC4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-4
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 4
    Urishiol oxidase 4
    Diphenol oxidase 4

Gene names

Name:	LAC4
Ordered Locus Names:	Os01g0842400, LOC_Os01g62480
ORF Names:	P0406G08.11, OsJ_003940

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 579	551	Laccase-4		PRO_0000291889
Regions
	Domain	36 – 152	117	Plastocyanin-like 1
	Domain	162 – 316	155	Plastocyanin-like 2
	Domain	429 – 563	135	Plastocyanin-like 3
Sites
	Metal binding	86	1	Copper 1 By similarity
	Metal binding	88	1	Copper 2 By similarity
	Metal binding	131	1	Copper 2 By similarity
	Metal binding	133	1	Copper 3 By similarity
	Metal binding	480	1	Copper 4 By similarity
	Metal binding	483	1	Copper 1 By similarity
	Metal binding	485	1	Copper 3 By similarity
	Metal binding	542	1	Copper 3 By similarity
	Metal binding	543	1	Copper 4 By similarity
	Metal binding	544	1	Copper 2 By similarity
	Metal binding	548	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Glycosylation	243	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	347	1	N-linked (GlcNAc...) Potential
	Glycosylation	386	1	N-linked (GlcNAc...) Potential
	Glycosylation	393	1	N-linked (GlcNAc...) Potential
	Glycosylation	403	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential
	Glycosylation	462	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5N9X2-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 2480C6A730DC02F3	FASTA	579	63,052
	10 20 30 40 50 60 MTMAISSALP SPLLLAASLL LLIVQAQGIT RHYEFNVQMA NATRLCNTKS MVTVNGQCPG 70 80 90 100 110 120 PELVAREGDR VVIRVTNNVA HNISLHWHGV RQVRTGWADG PAYITQCPIQ TGQSYVYNFT 130 140 150 160 170 180 VAGQRGTLWW HAHISWLRAT VYGALVILPK LGVPYPFPAP HKEVPVIFGE WWNADTEEVV 190 200 210 220 230 240 NQAVQTGGGP NVSDAFTING LPGPLYNCSA QDTFKLKVKP GKTYMLRLIN AALNEELFFA 250 260 270 280 290 300 VANHTLTVVE VDAVYVKPFT VDTLVISPGQ TTNVLLTAKP YYPGANFYMS AAPYSTARPG 310 320 330 340 350 360 TFGNTTVAGI LEYENPAMSP SAASFVKGLP LFKPTLPQLN DTDFVTNFTD KLRSLATPEY 370 380 390 400 410 420 PAAVPQSVDK RFFFTVGLGT LPCPANMTCQ GPNNTQMAAS MNNVSFVLPA RALLQSHFTG 430 440 450 460 470 480 LSSGVYAPDF PVAPLSPFNY TGTPPNNTNV KTGTKLLVLR YNTSVELVMQ DTSILGIESH 490 500 510 520 530 540 PLHLHGFNFF VIGQGFGNYD AVNDPAKFNL VDPVERNTVG VPAGGWVAIR FLADNPGVWF 550 560 570 MHCHLEAHTT WGLRMAWLVL DGSHPNQKLL PPPSDLPKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"Characterization and heterologous expression of laccase cDNAs from xylem tissues of yellow-poplar (Liriodendron tulipifera)." LaFayette P.R., Eriksson K.E., Dean J.F. Plant Mol. Biol. 40:23-35(1999) [PubMed: 10394942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-579. Strain: cv. Nipponbare. Tissue: Shoot.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003240 Genomic DNA. Translation: BAD81734.1. AP008207 Genomic DNA. Translation: BAF06686.1. CM000138 Genomic DNA. Translation: EAZ14115.1. Different initiation. AK068901 mRNA. No translation available. AF047697 mRNA. Translation: AAC04576.1.
PIR	T02752.
RefSeq	NP_001044772.1.
UniGene	Os.12346
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4327500.
KEGG	osa:4327500.
NMPDR	fig|39947.1.peg.7509.
Organism-specific databases
Gramene	Q5N9X2.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR03389. laccase. 1 hit.
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC4_ORYSJ

Accession

Primary (citable) accession number: Q5N9X2
Secondary accession number(s): A2ZZG8, O49232

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	February 1, 2005
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)