Reviewed, UniProtKB/Swiss-Prot Q5N9W4 (LAC5_ORYSJ)

Names and origin

Protein names

Recommended name:
    Putative laccase-5
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 5
    Urishiol oxidase 5
    Diphenol oxidase 5

Gene names

Name:	LAC5
Ordered Locus Names:	Os01g0843800, LOC_Os01g62600
ORF Names:	P0406G08.30, OsJ_003951

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.
Sequence caution	The sequence BAF06690.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 547	512	Putative laccase-5		PRO_0000291890
Regions
	Domain	43 – 159	117	Plastocyanin-like 1
	Domain	170 – 323	154	Plastocyanin-like 2
	Domain	408 – 531	124	Plastocyanin-like 3
Sites
	Metal binding	93	1	Copper 1 By similarity
	Metal binding	95	1	Copper 2 By similarity
	Metal binding	138	1	Copper 2 By similarity
	Metal binding	140	1	Copper 3 By similarity
	Metal binding	448	1	Copper 4 By similarity
	Metal binding	451	1	Copper 1 By similarity
	Metal binding	453	1	Copper 3 By similarity
	Metal binding	510	1	Copper 3 By similarity
	Metal binding	511	1	Copper 4 By similarity
	Metal binding	512	1	Copper 2 By similarity
	Metal binding	516	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	199	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Glycosylation	311	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	349	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Potential
	Glycosylation	395	1	N-linked (GlcNAc...) Potential
	Glycosylation	405	1	N-linked (GlcNAc...) Potential
	Glycosylation	430	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5N9W4-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 19E55D1604485C71	FASTA	547	59,891
	10 20 30 40 50 60 MGTPRGLRNA GSSSSACRFL AAFAVLLALP TLTAGLTRHY TFNVQMTNVT RLCVTKSIPT 70 80 90 100 110 120 VNGQFPGPKL VVREGDRLVV KVHNHMNYNV SFHWHGILQL RNGWADGPSY ITQCPIQGGG 130 140 150 160 170 180 SYVYDFTVTG QRGTLWWHAH FSWLRVHLYG PLVILPKRGE GFPFPRPYKE LPPIMFGEWF 190 200 210 220 230 240 NADTEAVINQ ALQTGAGPNI SDAYTFNGLP GPTYNCSSKD TYKVKVQPGR TYLLRLINSA 250 260 270 280 290 300 LNDELFFGIA NHTLTVVEAD ANYVKPFTAK TLVISPGQTM NLLLTTAPNP GSPVYAMAIA 310 320 330 340 350 360 PYTNTQGTFD NTTAVAVLEY APTRASATGN NNLPLPPLPR YNDTNAVANF SSKFRSLATA 370 380 390 400 410 420 RYPARVPRAV DRHVLFTVGL GTDPCPSNQT CQGPNGTKFA ASINNNSFVR PRVALLEAHC 430 440 450 460 470 480 QRRVVPLAFN TSVELVLQGT SIQGAESHPL HMHGFNFFVV GQGFGNYDPV NDPANYNLVD 490 500 510 520 530 540 PVERNTVSVP TGGWVAVRFL ADNPGVWLMH CHFDVHLSWG LSMAWLVNDG PLPSQKMLPP PSDLPKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003240 Genomic DNA. Translation: BAD81743.1. AP008207 Genomic DNA. Translation: BAF06690.1. Sequence problems. CM000138 Genomic DNA. Translation: EAZ14126.1.
RefSeq	NP_001044776.1.
UniGene	Os.79200
3D structure databases
HSSP	HSSP built from PDB template 1AOZ based on UniProtKB P37064.
ModBase	Search...
Genome annotation databases
GeneID	4327504.
KEGG	osa:4327504.
Organism-specific databases
Gramene	Q5N9W4.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR03389. laccase. 1 hit.
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC5_ORYSJ

Accession

Primary (citable) accession number: Q5N9W4
Secondary accession number(s): Q0JHT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)