Reviewed, UniProtKB/Swiss-Prot Q5N9Q7 (PTHM_ORYSJ)

Names and origin

Protein names

Recommended name:
    Peptidyl-tRNA hydrolase, mitochondrial
    EC=3.1.1.29
Alternative name(s):
    M-PTH

Gene names

Ordered Locus Names:	Os01g0693900, LOC_Os01g49900
ORF Names:	P0431H09.8

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity.
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the PTH family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Mitochondrion Potential
	Chain	46 – 250	205	Peptidyl-tRNA hydrolase, mitochondrial		PRO_0000280529
Experimental info
	Sequence conflict	72	1	R → L in AK066034. Ref.4
	Sequence conflict	230	1	E → V in AK066034. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q5N9Q7-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 4088D3F64ED08407	FASTA	250	26,959
	10 20 30 40 50 60 MRLLSGASAS RIPCPLLSLA RARARCLPVP ASATACRAAS SSAAAAAGDG GALKPWLFVG 70 80 90 100 110 120 LGNPGKVYQG TRHNVGFEMI DVIAEAEGIS LSSMQFKAMV GKGRIGDAPI MLAKPQTFMN 130 140 150 160 170 180 ASGESVGQLV SYFKIPLNQV LVMYDDLDLP FAKLRLLPKG GHGGHNGVRS IINHLKQNRD 190 200 210 220 230 240 FPRLRIGIGR PPGKMDPANF VLRPFNRKEQ EELDFAFHRG LEAVRIMALE GFNKSATYVN 250 TAQSSEMLNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003248 Genomic DNA. Translation: BAD81799.1. AP008207 Genomic DNA. Translation: BAF05862.1. AK066034 mRNA. No translation available. AK099894 mRNA. No translation available.
RefSeq	NP_001043948.1.
UniGene	Os.33607
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4323881.
KEGG	osa:4323881.
Organism-specific databases
Gramene	Q5N9Q7.
Family and domain databases
InterPro	IPR001328. Pept_tRNA_hydro. IPR018171. Pept_tRNA_hydro_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.1470. Pept_tRNA_hydro. 1 hit.
PANTHER	PTHR17224. Pept_tRNA_hydro. 1 hit.
Pfam	PF01195. Pept_tRNA_hydro. 1 hit. [Graphical view]
ProDom	PD005324. PeptRNAhydrolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00447. pth. 1 hit.
PROSITE	PS01195. PEPT_TRNA_HYDROL_1. 1 hit. PS01196. PEPT_TRNA_HYDROL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTHM_ORYSJ

Accession

Primary (citable) accession number: Q5N9Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	February 1, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)