Q5N9N2 (P2C09_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 9
Short name=OsPP2C09
EC=3.1.3.16

Gene names

Ordered Locus Names:	Os01g0846300, LOC_Os01g62760
ORF Names:	OsJ_003961, P0446B05.26-1, P0446B05.26-2, P0446B05.26-3

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence BAD81825.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAD81826.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	abscisic acid mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene negative regulation of abscisic acid mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene protein dephosphorylation Inferred from electronic annotation. Source: InterPro regulation of stomatal movement Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cold Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to water deprivation Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Probable protein phosphatase 2C 9		PRO_0000363255
Regions
	Transmembrane	15 – 37	23	Helical; Potential
	Domain	98 – 403	306	PP2C-like
	Compositional bias	38 – 42	5	Poly-Arg
Sites
	Metal binding	136	1	Manganese 1 By similarity
	Metal binding	136	1	Manganese 2 By similarity
	Metal binding	137	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	319	1	Manganese 2 By similarity
	Metal binding	401	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	72	1	R → H in AK065949. Ref.5
	Sequence conflict	334	1	V → M in AK065949. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q5N9N2 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: D1A6F1050916D251	FASTA	414	43,987
	10 20 30 40 50 60 MAEICCEEAM SPPATATAAV AAAVSASAAA AVSSAIDRRR RRMEMRRIRI ASDLELQAGE 70 80 90 100 110 120 DGRPGKRQRL ARTASGAPRP DEDSASERPS CGRTEEFPRY GVTAVCGRRR EMEDAVSIRP 130 140 150 160 170 180 DFLPASGKFH FYGVFDGHGC SHVATTCQDR MHEIVAEEHN KGASGEVAPW RDVMEKSFAR 190 200 210 220 230 240 MDGEVGNRAS TRSDDEPACP CEQQTPSRRD HAGSTAVVAV VSPTQVVVAN AGDSRAVISR 250 260 270 280 290 300 AGVPVALSVD HKPDRPDELE RIEAAGGRVI YWDGARVLGV LAMSRAIGDG YLKPYVTSEP 310 320 330 340 350 360 EVTVTERTDD DECLILASDG LWDVVTNEMA CEVVRACFHN NGPPAPAARP SGVPSSAEAA 370 380 390 400 410 ETENGGAASV KGISKAESSD KACSDAAMLL TKLALARRSA DNVSVVVVDL RRGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003251 Genomic DNA. Translation: BAD81824.1. AP003251 Genomic DNA. Translation: BAD81825.1. Sequence problems. AP003251 Genomic DNA. Translation: BAD81826.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF06702.1. CM000138 Genomic DNA. No translation available. AK065949 mRNA. No translation available.
RefSeq	NP_001044788.1. NM_001051323.1.
UniGene	Os.27906.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	Q5N9N2.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os01g62760.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g62760.1; LOC_Os01g62760.1; LOC_Os01g62760.
GeneID	4327465.
KEGG	osa:4327465.
Organism-specific databases
Gramene	Q5N9N2.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
KO	K14497.
OMA	SANCRCE.
ProtClustDB	CLSN2692044.
Gene expression databases
ArrayExpress	Q5N9N2.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C09_ORYSJ

Accession

Primary (citable) accession number: Q5N9N2
Secondary accession number(s): A2ZZI9, Q5N9N0, Q5N9N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 1, 2005
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program