Q5N8X6 (BGAL3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase 3
Short name=Lactase 3
EC=3.2.1.23

Gene names

Ordered Locus Names:	Os01g0875500, LOC_Os01g65460
ORF Names:	P0648C09.21, P0698A10.24

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	851 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.
Sequence caution	The sequence BAB86232.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 851	822	Beta-galactosidase 3		PRO_0000294154
Regions
	Domain	765 – 851	87	SUEL-type lectin
Sites
	Active site	194	1	Proton donor Potential
	Active site	263	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	35	1	N-linked (GlcNAc...) Potential
	Glycosylation	361	1	N-linked (GlcNAc...) Potential
	Glycosylation	475	1	N-linked (GlcNAc...) Potential
	Glycosylation	528	1	N-linked (GlcNAc...) Potential
	Glycosylation	533	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5N8X6 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: D2E70688E717B2FD	FASTA	851	94,710
	10 20 30 40 50 60 MAGASSYFSL RRLLLLLLPL VPLLGATTAA AAGANSSVTY DHRSLIISGR RRLLISTSIH 70 80 90 100 110 120 YPRSVPEMWP KLVAEAKDGG ADCVETYVFW NGHEPAQGQY YFEERFDLVR FAKIVKDAGL 130 140 150 160 170 180 YMILRIGPFV AAEWTFGGVP VWLHYAPGTV FRTNNEPFKS HMKRFTTYIV DMMKKEQFFA 190 200 210 220 230 240 SQGGHIILAQ VENEYGDMEQ AYGAGAKPYA MWAASMALAQ NTGVPWIMCQ QYDAPDPVIN 250 260 270 280 290 300 TCNSFYCDQF KPNSPTKPKF WTENWPGWFQ TFGESNPHRP PEDVAFSVAR FFGKGGSLQN 310 320 330 340 350 360 YYVYHGGTNF GRTTGGPFIT TSYDYDAPID EYGLRRLPKW AHLRDLHKSI KLGEHTLLYG 370 380 390 400 410 420 NSSFVSLGPQ QEADVYTDQS GGCVAFLSNV DSEKDKVVTF QSRSYDLPAW SVSILPDCKN 430 440 450 460 470 480 VAFNTAKVRS QTLMMDMVPA NLESSKVDGW SIFREKYGIW GNIDLVRNGF VDHINTTKDS 490 500 510 520 530 540 TDYLWYTTSF DVDGSHLAGG NHVLHIESKG HAVQAFLNNE LIGSAYGNGS KSNFSVEMPV 550 560 570 580 590 600 NLRAGKNKLS LLSMTVGLQN GGPMYEWAGA GITSVKISGM ENRIIDLSSN KWEYKIGLEG 610 620 630 640 650 660 EYYSLFKADK GKDIRWMPQS EPPKNQPMTW YKVNVDVPQG DDPVGLDMQS MGKGLAWLNG 670 680 690 700 710 720 NAIGRYWPRI SPVSDRCTSS CDYRGTFSPN KCRRGCGQPT QRWYHVPRSW FHPSGNTLVI 730 740 750 760 770 780 FEEKGGDPTK ITFSRRTVAS VCSFVSEHYP SIDLESWDRN TQNDGRDAAK VQLSCPKGKS 790 800 810 820 830 840 ISSVKFVSFG NPSGTCRSYQ QGSCHHPNSI SVVEKACLNM NGCTVSLSDE GFGEDLCPGV 850 TKTLAIEADC S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003297 Genomic DNA. Translation: BAD82087.1. AP003922 Genomic DNA. Translation: BAB86232.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF06878.1.
RefSeq	NP_001044964.1. NM_001051499.1.
UniGene	Os.9892.
3D structure databases
ProteinModelPortal	Q5N8X6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5N8X6.
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q5N8X6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4324777.
KEGG	osa:4324777.
Organism-specific databases
Gramene	Q5N8X6.
Phylogenomic databases
GeneTree	EPGT00070000027970.
HOGENOM	HBG318019.
PhylomeDB	Q5N8X6.
ProtClustDB	CLSN2692124.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR006104. Glyco_hydro_2_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. IPR000922. Lectin_gal-bd_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL3_ORYSJ

Accession

Primary (citable) accession number: Q5N8X6
Secondary accession number(s): Q8RYX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	February 1, 2005
Last modified:	December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program