Reviewed, UniProtKB/Swiss-Prot Q5N7A3 (LAC6_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-6
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 6
    Urishiol oxidase 6
    Diphenol oxidase 6

Gene names

Name:	LAC6
Ordered Locus Names:	LOC_Os01g63180
ORF Names:	P0529H11.21, OsJ_003986

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 580	558	Laccase-6		PRO_0000291891
Regions
	Domain	30 – 148	119	Plastocyanin-like 1
	Domain	158 – 317	160	Plastocyanin-like 2
	Domain	424 – 564	141	Plastocyanin-like 3
Sites
	Metal binding	82	1	Copper 1 By similarity
	Metal binding	84	1	Copper 2 By similarity
	Metal binding	127	1	Copper 2 By similarity
	Metal binding	129	1	Copper 3 By similarity
	Metal binding	480	1	Copper 4 By similarity
	Metal binding	483	1	Copper 1 By similarity
	Metal binding	485	1	Copper 3 By similarity
	Metal binding	543	1	Copper 3 By similarity
	Metal binding	544	1	Copper 4 By similarity
	Metal binding	545	1	Copper 2 By similarity
	Metal binding	549	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Glycosylation	385	1	N-linked (GlcNAc...) Potential
	Glycosylation	397	1	N-linked (GlcNAc...) Potential
	Glycosylation	462	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5N7A3-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: C920CD4B9452D017	FASTA	580	62,569
	10 20 30 40 50 60 MSCSWMIPVF AILAFVASAA QADVVEHTFN VATLSLPRIC QPGNTSVTAV NGRVPGPQVE 70 80 90 100 110 120 AREGDTVVIH VINDSPYNVT VHWHGVFQRG TPWADGPAMV TQCPIRPGHR YTYRFAVAGQ 130 140 150 160 170 180 EGTLWWHAHS SYMRATVYGA LVIRPRRAGG YPFPTPYEEK TVLLGEWWNG DPVALESQSF 190 200 210 220 230 240 STGIPAPNAD AYTINGMPGD SYLCPETTNR IAKFEVRRDK TYLLRIINAA LNTAFFFKVA 250 260 270 280 290 300 GHTFTVVAAD ASYTEPYATD VIVIAPGQTV DALMAADASP GCYHMAISSY QSAIPFPPRP 310 320 330 340 350 360 AGFNGNTSTA IVEYVDATAT TDAGSPVLPV MPKPNDTYTA NQFYTSLTAL IRPGRRTVPL 370 380 390 400 410 420 TVDTRMLVTV GLGFSSCQPE QTQCNRSAPV VLANMNNVSF ALPNTVSMLE ALYRNTADGV 430 440 450 460 470 480 YTRDFPDQPP VAFDYTSRGL LGNSPLASTG SPSTKVKTLR YNATVEMVLQ NTALVGLESH 490 500 510 520 530 540 PMHLHGFNFF VVAQGFGNND GEAAGAGEFN LVNPQERNTV AVPTGGWAVI RFVADNPGMW 550 560 570 580 AMHCHIDSHF AIGLAMVFEV ESGPTPGTTL PPPPPDLPQC

References

[1]

"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003242 Genomic DNA. Translation: BAD81778.1. AP004072 Genomic DNA. Translation: BAD82646.1. CM000138 Genomic DNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1AOZ based on UniProtKB P37064.
ModBase	Search...
Organism-specific databases
Gramene	Q5N7A3.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR03389. laccase. 1 hit.
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC6_ORYSJ

Accession

Primary (citable) accession number: Q5N7A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)