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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciSELO269084:GCDQ-244-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:syc0238_d
OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Taxonomic identifieri269084 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
Proteomesi
  • UP000001175 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117282Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269084.syc0238_d.

Structurei

3D structure databases

ProteinModelPortaliQ5N5J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5N5J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAISQPVIV IGAGLAGTEA AWQIAEAGVP VILYEMRPQR QSPAHHSESF
60 70 80 90 100
AELVCSNSFG AMASDRAAGL LHEELRRLGS LVFSKASEHQ VPAGGALAVD
110 120 130 140 150
RALFSEDLTR TVADHPLVEI RREELRSLPT EGIVVLCTGP LTSPDLAEDL
160 170 180 190 200
QRFTGQDYCS FFDAASPIVT GESIDQAIAF RASRYDKGEA AYLNCPLNRD
210 220 230 240 250
QYLAFREALV TAEQAELKDF EQESAKFFEG CLPIEELARR GEDTMRYGPL
260 270 280 290 300
KPVGLFDARL GDWRDPENRS RRPYAIVQLR QEDRAGNLWN LVGFQTNLRW
310 320 330 340 350
GEQKRIFQMI PGLSQAEFVR FGVMHRNTFV NAPQLLDASL QFRQRPTLLA
360 370 380 390 400
AGQLIGTEGY SAAVAGGWLA GTNAARLALG RSPLVLPDTL VSGSLFRFIS
410 420 430 440 450
SAEPKYFQPM PPNFGILPNL EQPPRNKKDR YAAYRDRALQ DLRDWQQTHA
460
IGNLSPSTGL ATTAIA
Length:466
Mass (Da):51,388
Last modified:February 1, 2005 - v1
Checksum:iD8A05945BBCC7582
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA. Translation: BAD78428.1.
RefSeqiWP_011242552.1. NC_006576.1.

Genome annotation databases

EnsemblBacteriaiBAD78428; BAD78428; syc0238_d.
KEGGisyc:syc0238_d.
PATRICi32486010. VBISynElo117686_0282.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA. Translation: BAD78428.1.
RefSeqiWP_011242552.1. NC_006576.1.

3D structure databases

ProteinModelPortaliQ5N5J0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269084.syc0238_d.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD78428; BAD78428; syc0238_d.
KEGGisyc:syc0238_d.
PATRICi32486010. VBISynElo117686_0282.

Phylogenomic databases

eggNOGiCOG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciSELO269084:GCDQ-244-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
    Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
    Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Entry informationi

Entry nameiTRMFO_SYNP6
AccessioniPrimary (citable) accession number: Q5N5J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: February 1, 2005
Last modified: January 20, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.