Q5N4W5 (GLGB_SYNP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	syc0464_c

Organism

Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) [Complete proteome] [HAMAP]

Taxonomic identifier

269084 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 774	774	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000188755
Sites
	Active site	440	1	Nucleophile By similarity
	Active site	493	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5N4W5 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: C4C8885EF103CCE4	FASTA	774	89,195
	10 20 30 40 50 60 MTGTTPLPSS SLSVEQVNRI ASNQEQNPFD ILGPHPYEHE GQAGWVIRAY LPEAQEAAVI 70 80 90 100 110 120 CPALRREFAM HPVHHPHFFE TWVPEETLEI YQLRITEGER ERIIYDPYAF RSPLLTDYDI 130 140 150 160 170 180 HLFAEGNHHR IYEKLGAHPC ELENVAGVNF AVWAPSARNV SILGDFNSWD GRKHQMARRS 190 200 210 220 230 240 NGIWELFIPE LTVGAAYKYE IKNYDGHIYE KSDPYGFQQE VRPKTASIVA DLDRYTWGDA 250 260 270 280 290 300 DWLERRRHQE PLRQPISVYE VHLGSWMHAS SDAIATDAQG KPLPPVPVAD LKPGARFLTY 310 320 330 340 350 360 RELADRLIPY VLDLGYSHIE LLPIAEHPFD GSWGYQVTGY YAATSRYGSP EDFMYFVDRC 370 380 390 400 410 420 HQNGIGVILD WVPGHFPKDG HGLAFFDGTH LYEHADSRQG EHREWGTLVF NYGRHEVRNF 430 440 450 460 470 480 LAANALFWFD KYHIDGIRVD AVASMLYLDY NRKEGEWIPN EYGGRENIEA ADFLRQVNHL 490 500 510 520 530 540 IFSYFPGALS IAEESTSWPM VSWPTYVGGL GFNLKWNMGW MHDMLDYFSM DPWFRQFHQN 550 560 570 580 590 600 NVTFSIWYAF SENFMLALSH DEVVHGKSNL IGKMPGDEWQ KFANLRCLLG YMFTHPGKKT 610 620 630 640 650 660 LFMGMEFGQW AEWNVWGDLE WHLLQYEPHQ GLKQFVKDLN HLYRNAPALY SEDCNQAGFE 670 680 690 700 710 720 WIDCSDNRHS IVSFIRRAHE SDRFLVVVCN FTPQPHAHYR IGVPVAGFYR EIFNSDARSY 730 740 750 760 770 GGSNMGNLGG KWTDEWSCHN RPYSLDLCLP PLTTLVLELA SGPESLSEAA NSPL

References

[1]

"Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization." Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M. Photosyn. Res. 93:55-67(2007) [PubMed: 17211581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008231 Genomic DNA. Translation: BAD78654.1.
RefSeq	YP_171174.1. NC_006576.1.
3D structure databases
ProteinModelPortal	Q5N4W5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5N4W5.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3198206.
GenomeReviews	Gene locus syc0464_c in contig AP008231_GR.
KEGG	syc:syc0464_c.
PATRIC	32486520. VBISynElo117686_0534.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	DGTCLYE.
PhylomeDB	Q5N4W5.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	SELO269084:SYC0464_C-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_SYNP6

Accession

Primary (citable) accession number: Q5N4W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program