Q5N2S7 (ISPE_SYNP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	syc1203_d

Organism

Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) [Complete proteome] [HAMAP]

Taxonomic identifier

269084 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235144
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	137	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5N2S7 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 73A524A99939DD89	FASTA	314	33,747
	10 20 30 40 50 60 MKTLTLVAPA KINFYLEILG DRPDGFHELA MVMQSIALGD RLHLRQRQGQ GFQLGCDRAD 70 80 90 100 110 120 LEVDERNLIL KAAQRLQRSF PQIGGVDFFL EKRIPIGAGL AGGSTDGAAA LVGLDIFSGL 130 140 150 160 170 180 GLTQPELEAI AAELGSDMPF CVAGGTQLCT SRGEQLTPLP PLPSLSLVLA KFESLSVSTP 190 200 210 220 230 240 WAYGRYREQF GDRYLQTEPA RRDRLQALHA GPLLQAMSAG DPVAIAQHLR NDLEAVVLPE 250 260 270 280 290 300 YPQVAELRSV LGNCSGILAA QMSGSGPSVF GIAENPTAAQ AAVEQVRQAI ADPDLKLWVT 310 QTISHGIQTE TLLR

References

[1]

"Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization." Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M. Photosyn. Res. 93:55-67(2007) [PubMed: 17211581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008231 Genomic DNA. Translation: BAD79393.1.
RefSeq	YP_171913.1. NC_006576.1.
3D structure databases
ProteinModelPortal	Q5N2S7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5N2S7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3197933.
GenomeReviews	Gene locus syc1203_d in contig AP008231_GR.
KEGG	syc:syc1203_d.
PATRIC	32488271. VBISynElo117686_1394.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	CQLARMS.
PhylomeDB	Q5N2S7.
ProtClustDB	PRK02534.
Enzyme and pathway databases
BioCyc	SELO269084:SYC1203_D-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_SYNP6

Accession

Primary (citable) accession number: Q5N2S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program