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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi66Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei96SubstrateUniRule annotation1
Metal bindingi157Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei185SubstrateUniRule annotation1
Active sitei187Proton donorUniRule annotation1
Binding sitei250SubstrateUniRule annotation1
Metal bindingi288Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei379SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097
UPA00059; UER00105

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Short name:
HMBPP reductaseUniRule annotation
Gene namesi
Name:ispHUniRule annotation
Synonyms:lytB
Ordered Locus Names:syc1431_d
OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Taxonomic identifieri269084 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001175 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001288781 – 3984-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST398

Interactioni

Protein-protein interaction databases

STRINGi269084.syc1431_d

Structurei

3D structure databases

SMRiQ5N249
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni317 – 319Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RAJ Bacteria
COG0761 LUCA
HOGENOMiHOG000240388
KOiK03527
OMAiHNKYVVD
OrthoDBiPOG091H038O

Family and domain databases

CDDicd13944 lytB_ispH, 1 hit
HAMAPiMF_00191 IspH, 1 hit
InterProiView protein in InterPro
IPR003451 LytB/IspH
PfamiView protein in Pfam
PF02401 LYTB, 1 hit
TIGRFAMsiTIGR00216 ispH_lytB, 1 hit

Sequencei

Sequence statusi: Complete.

Q5N249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTKAFKRAL HQSDRYNRKG FGKTTDVSGA LESAYQSDLI QSLRQNGYRL
60 70 80 90 100
QRGEITIRLA EAFGFCWGVE RAVAIAYETR QHFPQERIWI TNEIIHNPSV
110 120 130 140 150
NQHLREMSVE FIPCERGEKD FSVVDRGDVV ILPAFGASVQ EMQLLDEKGC
160 170 180 190 200
HIVDTTCPWV SKVWNTVEKH KRGAHTSIIH GKYNHEETVA TSSFAETYLV
210 220 230 240 250
VLNLEQAQYV CDYILNGGDR DEFMTRFGKA CSAGFDPDRD LERIGIANQT
260 270 280 290 300
TMLKSETEAI GKLFERTLLK KYGPQALNDH FLAFNTICDA TQERQDAMFQ
310 320 330 340 350
LVEEPLDLIV VIGGFNSSNT THLQEIAIER QIPSFHIDAA ERIGPGNRIE
360 370 380 390
HKPLHTDLTT TEPWLPAGPL TIGITSGAST PDKVVEDVIE RLFDLQRS
Length:398
Mass (Da):44,941
Last modified:February 1, 2005 - v1
Checksum:iB79CFEC204262821
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA Translation: BAD79621.1
RefSeqiWP_011243743.1, NC_006576.1

Genome annotation databases

EnsemblBacteriaiBAD79621; BAD79621; syc1431_d
KEGGisyc:syc1431_d

Similar proteinsi

Entry informationi

Entry nameiISPH_SYNP6
AccessioniPrimary (citable) accession number: Q5N249
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: February 1, 2005
Last modified: March 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health