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Q5N1G1

- SYI_SYNP6

UniProt

Q5N1G1 - SYI_SYNP6

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Protein
Isoleucine--tRNA ligase
Gene
ileS, syc1669_d
Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity
Metal bindingi943 – 9431Zinc By similarity
Metal bindingi946 – 9461Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSELO269084:GCDQ-1714-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:syc1669_d
OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Taxonomic identifieri269084 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001175: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 954954Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098490Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi269084.syc1669_d.

Structurei

3D structure databases

ProteinModelPortaliQ5N1G1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5N1G1-1 [UniParc]FASTAAdd to Basket

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MAEVSAYKDT LNLLQTPFNM RANAPVREPE IQQFWADRQI YETLSRQNPG    50
APFVLHDGPP YANGALHMGH ALNKTLKDII NKYQLLQGRK VRYVPGWDCH 100
GLPIELKVLQ ELSSEERRNL TPLTLRQKAK AYALAQVEQQ SQSFQRYGVW 150
ADWDAPYLTL TPEYEAAQID VFGQMVLKGY IYRGLKPVHW SPSSRTALAE 200
AELEYPDGHT SRSIYVAMPI VQLSEAAQPL LGNYANLALA IWTTTPWTIP 250
ANLAVAVNGE LTYAVVQAGD CHLIVAAELA ESLSKTFATE LTVLATFPGS 300
VLEHSRYRHP LYDRESPVVI GGDYITTESG TGLVHTAPGH GQDDFIVGNR 350
YGLEVFCPVD DKGDFTAAVG DRLVGKNVLK DANAAVIEWL TEVGALLKEE 400
SYAHRYPYDW RTKKPTIFRA TEQWFASVEG FREQALQAIA EVDWIPAQGE 450
NRITSMVSER SDWCISRQRT WGVPIPVFYD EESGEALLNA ETIAHVRAIV 500
AERGSDAWWE LDVADLLPEP YRSNGRRYRK GTDTMDVWFD SGSSWAAVAS 550
QREGLHYPAD LYLEGSDQHR GWFQSSLLTS VACNGHAPYR RVLTHGFALD 600
EKGRKMSKSL GNVVDPAIVI NGGKDQKQEP PYGADVLRLW VSSVDYSSDV 650
PIGKNILKQM ADVYRKIRNT ARFLLGNLHD FDPAKDALPW EKLPELDRYL 700
LHRLREVILE IQDAFESFQF FRFFQTVQNF CVVDLSNFYL DIGKDRLYIS 750
APDSLRRRSC QTVLAICVEA LATAIAPVLS HMAEDIWQSL PYPARTKSVF 800
QAGWVQLQDD WNQPELAAKW QQLRDLRSEV NKVLEQARRD QAIGSSLEAK 850
LQLWVADSDW RAALADRNPA DSLSGTAVDD LRYLFLVSQV ELRDQPTGLT 900
EAKYHAQTED WAIAVVDAEG QKCDRCWNYS TTVGQSSEHP DLCDRCVSAL 950
QGTF 954
Length:954
Mass (Da):107,414
Last modified:February 1, 2005 - v1
Checksum:iDC282BC4C1431307
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008231 Genomic DNA. Translation: BAD79859.1.
RefSeqiWP_011243979.1. NC_006576.1.
YP_172379.1. NC_006576.1.

Genome annotation databases

EnsemblBacteriaiBAD79859; BAD79859; syc1669_d.
GeneIDi3197837.
KEGGisyc:syc1669_d.
PATRICi32489338. VBISynElo117686_1910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008231 Genomic DNA. Translation: BAD79859.1 .
RefSeqi WP_011243979.1. NC_006576.1.
YP_172379.1. NC_006576.1.

3D structure databases

ProteinModelPortali Q5N1G1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269084.syc1669_d.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD79859 ; BAD79859 ; syc1669_d .
GeneIDi 3197837.
KEGGi syc:syc1669_d.
PATRICi 32489338. VBISynElo117686_1910.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SELO269084:GCDQ-1714-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
    Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
    Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Entry informationi

Entry nameiSYI_SYNP6
AccessioniPrimary (citable) accession number: Q5N1G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: September 3, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi