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Q5N1G1

- SYI_SYNP6

UniProt

Q5N1G1 - SYI_SYNP6

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi943 – 9431ZincUniRule annotation
Metal bindingi946 – 9461ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSELO269084:GCDQ-1714-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:syc1669_d
OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Taxonomic identifieri269084 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001175: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 954954Isoleucine--tRNA ligasePRO_0000098490Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269084.syc1669_d.

Structurei

3D structure databases

ProteinModelPortaliQ5N1G1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5N1G1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEVSAYKDT LNLLQTPFNM RANAPVREPE IQQFWADRQI YETLSRQNPG
60 70 80 90 100
APFVLHDGPP YANGALHMGH ALNKTLKDII NKYQLLQGRK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ ELSSEERRNL TPLTLRQKAK AYALAQVEQQ SQSFQRYGVW
160 170 180 190 200
ADWDAPYLTL TPEYEAAQID VFGQMVLKGY IYRGLKPVHW SPSSRTALAE
210 220 230 240 250
AELEYPDGHT SRSIYVAMPI VQLSEAAQPL LGNYANLALA IWTTTPWTIP
260 270 280 290 300
ANLAVAVNGE LTYAVVQAGD CHLIVAAELA ESLSKTFATE LTVLATFPGS
310 320 330 340 350
VLEHSRYRHP LYDRESPVVI GGDYITTESG TGLVHTAPGH GQDDFIVGNR
360 370 380 390 400
YGLEVFCPVD DKGDFTAAVG DRLVGKNVLK DANAAVIEWL TEVGALLKEE
410 420 430 440 450
SYAHRYPYDW RTKKPTIFRA TEQWFASVEG FREQALQAIA EVDWIPAQGE
460 470 480 490 500
NRITSMVSER SDWCISRQRT WGVPIPVFYD EESGEALLNA ETIAHVRAIV
510 520 530 540 550
AERGSDAWWE LDVADLLPEP YRSNGRRYRK GTDTMDVWFD SGSSWAAVAS
560 570 580 590 600
QREGLHYPAD LYLEGSDQHR GWFQSSLLTS VACNGHAPYR RVLTHGFALD
610 620 630 640 650
EKGRKMSKSL GNVVDPAIVI NGGKDQKQEP PYGADVLRLW VSSVDYSSDV
660 670 680 690 700
PIGKNILKQM ADVYRKIRNT ARFLLGNLHD FDPAKDALPW EKLPELDRYL
710 720 730 740 750
LHRLREVILE IQDAFESFQF FRFFQTVQNF CVVDLSNFYL DIGKDRLYIS
760 770 780 790 800
APDSLRRRSC QTVLAICVEA LATAIAPVLS HMAEDIWQSL PYPARTKSVF
810 820 830 840 850
QAGWVQLQDD WNQPELAAKW QQLRDLRSEV NKVLEQARRD QAIGSSLEAK
860 870 880 890 900
LQLWVADSDW RAALADRNPA DSLSGTAVDD LRYLFLVSQV ELRDQPTGLT
910 920 930 940 950
EAKYHAQTED WAIAVVDAEG QKCDRCWNYS TTVGQSSEHP DLCDRCVSAL

QGTF
Length:954
Mass (Da):107,414
Last modified:February 1, 2005 - v1
Checksum:iDC282BC4C1431307
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA. Translation: BAD79859.1.
RefSeqiWP_011243979.1. NC_006576.1.
YP_172379.1. NC_006576.1.

Genome annotation databases

EnsemblBacteriaiBAD79859; BAD79859; syc1669_d.
GeneIDi3197837.
KEGGisyc:syc1669_d.
PATRICi32489338. VBISynElo117686_1910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA. Translation: BAD79859.1 .
RefSeqi WP_011243979.1. NC_006576.1.
YP_172379.1. NC_006576.1.

3D structure databases

ProteinModelPortali Q5N1G1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269084.syc1669_d.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD79859 ; BAD79859 ; syc1669_d .
GeneIDi 3197837.
KEGGi syc:syc1669_d.
PATRICi 32489338. VBISynElo117686_1910.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SELO269084:GCDQ-1714-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
    Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
    Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Entry informationi

Entry nameiSYI_SYNP6
AccessioniPrimary (citable) accession number: Q5N1G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: November 26, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3