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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi84Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi156Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi160Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi163Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:syc1731_c
OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Taxonomic identifieri269084 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001175 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003745931 – 452tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST452

Proteomic databases

PRIDEiQ5N199.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269084.syc1731_c.

Structurei

3D structure databases

ProteinModelPortaliQ5N199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 121MTTase N-terminalUniRule annotationAdd BLAST117
Domaini382 – 446TRAMUniRule annotationAdd BLAST65

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiIPMDLIL.
OrthoDBiPOG091H00LG.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5N199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPRRYHIT TFGCQMNKAD SERMAGILED LGYIWSEEAN DADLVLYNTC
60 70 80 90 100
TIRDNAEQKV YSYLGRQAER KRQQPDLTLI VAGCVAQQEG ESLLRRVPEL
110 120 130 140 150
DLVMGPQHAN RLADLLAQVE AGSQVVATEE VEIAEDITQP RRDSTITAWV
160 170 180 190 200
NVIYGCNERC TYCVVPNVRG REQSREPAAI RAEIEQLAAQ GYREITLLGQ
210 220 230 240 250
NIDAYGRDLP GSTPEGRHLH TLTDLLYTIH DVPGIERIRF ATSHPRYFTE
260 270 280 290 300
RLIRACAELP KVCEYFHIPF QSGDNDVLKA MARGYTVERY LRIVEQIRDI
310 320 330 340 350
IPDAAISADA IVAFPGETEE QFENTLKLVE QVGFDLVNTA AYSPRPGTPA
360 370 380 390 400
ANAPNQLSEE VKQDRLQRLN HLVAQMAADR SQRYLGRTEE VLIEATNPRN
410 420 430 440 450
PQQVMGRTRT NRLVFCDGTI AQLEGQLVPV RITETRAFSL TGQILSPVAA

GC
Length:452
Mass (Da):50,656
Last modified:February 1, 2005 - v1
Checksum:i6EF09532ED2FA30C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA. Translation: BAD79921.1.
RefSeqiWP_011244041.1. NC_006576.1.

Genome annotation databases

EnsemblBacteriaiBAD79921; BAD79921; syc1731_c.
KEGGisyc:syc1731_c.
PATRICi32489474. VBISynElo117686_1977.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA. Translation: BAD79921.1.
RefSeqiWP_011244041.1. NC_006576.1.

3D structure databases

ProteinModelPortaliQ5N199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269084.syc1731_c.

Proteomic databases

PRIDEiQ5N199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD79921; BAD79921; syc1731_c.
KEGGisyc:syc1731_c.
PATRICi32489474. VBISynElo117686_1977.

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiIPMDLIL.
OrthoDBiPOG091H00LG.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIAB_SYNP6
AccessioniPrimary (citable) accession number: Q5N199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: February 1, 2005
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.