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Q5N199 (MIAB_SYNP6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

EC=2.8.4.3
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene names
Name:miaB
Ordered Locus Names:syc1731_c
OrganismSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) [Complete proteome] [HAMAP]
Taxonomic identifier269084 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864

Catalytic activity

N(6)-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine. HAMAP-Rule MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01864

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01864.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

transferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 452452tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase HAMAP-Rule MF_01864
PRO_0000374593

Regions

Domain5 – 121117MTTase N-terminal
Domain382 – 44665TRAM

Sites

Metal binding141Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding841Iron-sulfur (4Fe-4S) By similarity
Metal binding1561Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1601Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1631Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5N199 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 6EF09532ED2FA30C

FASTA45250,656
        10         20         30         40         50         60 
MSTPRRYHIT TFGCQMNKAD SERMAGILED LGYIWSEEAN DADLVLYNTC TIRDNAEQKV 

        70         80         90        100        110        120 
YSYLGRQAER KRQQPDLTLI VAGCVAQQEG ESLLRRVPEL DLVMGPQHAN RLADLLAQVE 

       130        140        150        160        170        180 
AGSQVVATEE VEIAEDITQP RRDSTITAWV NVIYGCNERC TYCVVPNVRG REQSREPAAI 

       190        200        210        220        230        240 
RAEIEQLAAQ GYREITLLGQ NIDAYGRDLP GSTPEGRHLH TLTDLLYTIH DVPGIERIRF 

       250        260        270        280        290        300 
ATSHPRYFTE RLIRACAELP KVCEYFHIPF QSGDNDVLKA MARGYTVERY LRIVEQIRDI 

       310        320        330        340        350        360 
IPDAAISADA IVAFPGETEE QFENTLKLVE QVGFDLVNTA AYSPRPGTPA ANAPNQLSEE 

       370        380        390        400        410        420 
VKQDRLQRLN HLVAQMAADR SQRYLGRTEE VLIEATNPRN PQQVMGRTRT NRLVFCDGTI 

       430        440        450 
AQLEGQLVPV RITETRAFSL TGQILSPVAA GC 

« Hide

References

[1]"Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008231 Genomic DNA. Translation: BAD79921.1.
RefSeqYP_172441.1. NC_006576.1.

3D structure databases

ProteinModelPortalQ5N199.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269084.syc1731_c.

Proteomic databases

PRIDEQ5N199.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD79921; BAD79921; syc1731_c.
GeneID3198429.
KEGGsyc:syc1731_c.
PATRIC32489474. VBISynElo117686_1977.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0621.
HOGENOMHOG000224767.
KOK06168.
OMAIEVYKDT.
OrthoDBEOG6P5ZD8.

Enzyme and pathway databases

BioCycSELO269084:GCDQ-1778-MONOMER.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIAB_SYNP6
AccessionPrimary (citable) accession number: Q5N199
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families