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Q5N199

- MIAB_SYNP6

UniProt

Q5N199 - MIAB_SYNP6

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Protein
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Gene
miaB, syc1731_c
Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi84 – 841Iron-sulfur (4Fe-4S) By similarity
Metal bindingi156 – 1561Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi160 – 1601Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi163 – 1631Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSELO269084:GCDQ-1778-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (EC:2.8.4.3)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene namesi
Name:miaB
Ordered Locus Names:syc1731_c
OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Taxonomic identifieri269084 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001175: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation
PRO_0000374593Add
BLAST

Proteomic databases

PRIDEiQ5N199.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi269084.syc1731_c.

Structurei

3D structure databases

ProteinModelPortaliQ5N199.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 121117MTTase N-terminal
Add
BLAST
Domaini382 – 44665TRAM
Add
BLAST

Sequence similaritiesi

Contains 1 TRAM domain.

Phylogenomic databases

eggNOGiCOG0621.
HOGENOMiHOG000224767.
KOiK06168.
OMAiIEVYKDT.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5N199-1 [UniParc]FASTAAdd to Basket

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MSTPRRYHIT TFGCQMNKAD SERMAGILED LGYIWSEEAN DADLVLYNTC    50
TIRDNAEQKV YSYLGRQAER KRQQPDLTLI VAGCVAQQEG ESLLRRVPEL 100
DLVMGPQHAN RLADLLAQVE AGSQVVATEE VEIAEDITQP RRDSTITAWV 150
NVIYGCNERC TYCVVPNVRG REQSREPAAI RAEIEQLAAQ GYREITLLGQ 200
NIDAYGRDLP GSTPEGRHLH TLTDLLYTIH DVPGIERIRF ATSHPRYFTE 250
RLIRACAELP KVCEYFHIPF QSGDNDVLKA MARGYTVERY LRIVEQIRDI 300
IPDAAISADA IVAFPGETEE QFENTLKLVE QVGFDLVNTA AYSPRPGTPA 350
ANAPNQLSEE VKQDRLQRLN HLVAQMAADR SQRYLGRTEE VLIEATNPRN 400
PQQVMGRTRT NRLVFCDGTI AQLEGQLVPV RITETRAFSL TGQILSPVAA 450
GC 452
Length:452
Mass (Da):50,656
Last modified:February 1, 2005 - v1
Checksum:i6EF09532ED2FA30C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008231 Genomic DNA. Translation: BAD79921.1.
RefSeqiWP_011244041.1. NC_006576.1.
YP_172441.1. NC_006576.1.

Genome annotation databases

EnsemblBacteriaiBAD79921; BAD79921; syc1731_c.
GeneIDi3198429.
KEGGisyc:syc1731_c.
PATRICi32489474. VBISynElo117686_1977.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008231 Genomic DNA. Translation: BAD79921.1 .
RefSeqi WP_011244041.1. NC_006576.1.
YP_172441.1. NC_006576.1.

3D structure databases

ProteinModelPortali Q5N199.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269084.syc1731_c.

Proteomic databases

PRIDEi Q5N199.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD79921 ; BAD79921 ; syc1731_c .
GeneIDi 3198429.
KEGGi syc:syc1731_c.
PATRICi 32489474. VBISynElo117686_1977.

Phylogenomic databases

eggNOGi COG0621.
HOGENOMi HOG000224767.
KOi K06168.
OMAi IEVYKDT.
OrthoDBi EOG6P5ZD8.

Enzyme and pathway databases

BioCyci SELO269084:GCDQ-1778-MONOMER.

Family and domain databases

Gene3Di 3.80.30.20. 1 hit.
HAMAPi MF_01864. tRNA_metthiotr_MiaB.
InterProi IPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view ]
PANTHERi PTHR11918. PTHR11918. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
PROSITEi PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
    Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
    Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Entry informationi

Entry nameiMIAB_SYNP6
AccessioniPrimary (citable) accession number: Q5N199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: February 1, 2005
Last modified: September 3, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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