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Q5N199

- MIAB_SYNP6

UniProt

Q5N199 - MIAB_SYNP6

Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

    Catalytic activityi

    N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi14 – 141Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi84 – 841Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi156 – 1561Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi160 – 1601Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi163 – 1631Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. transferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSELO269084:GCDQ-1778-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
    Alternative name(s):
    (Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
    tRNA-i(6)A37 methylthiotransferaseUniRule annotation
    Gene namesi
    Name:miaBUniRule annotation
    Ordered Locus Names:syc1731_c
    OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
    Taxonomic identifieri269084 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000001175: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000374593Add
    BLAST

    Proteomic databases

    PRIDEiQ5N199.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi269084.syc1731_c.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5N199.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 121117MTTase N-terminalUniRule annotationAdd
    BLAST
    Domaini382 – 44665TRAMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
    Contains 1 MTTase N-terminal domain.UniRule annotation
    Contains 1 TRAM domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0621.
    HOGENOMiHOG000224767.
    KOiK06168.
    OMAiIEVYKDT.
    OrthoDBiEOG6P5ZD8.

    Family and domain databases

    Gene3Di3.80.30.20. 1 hit.
    HAMAPiMF_01864. tRNA_metthiotr_MiaB.
    InterProiIPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view]
    PANTHERiPTHR11918. PTHR11918. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
    PROSITEiPS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5N199-1 [UniParc]FASTAAdd to Basket

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    MSTPRRYHIT TFGCQMNKAD SERMAGILED LGYIWSEEAN DADLVLYNTC    50
    TIRDNAEQKV YSYLGRQAER KRQQPDLTLI VAGCVAQQEG ESLLRRVPEL 100
    DLVMGPQHAN RLADLLAQVE AGSQVVATEE VEIAEDITQP RRDSTITAWV 150
    NVIYGCNERC TYCVVPNVRG REQSREPAAI RAEIEQLAAQ GYREITLLGQ 200
    NIDAYGRDLP GSTPEGRHLH TLTDLLYTIH DVPGIERIRF ATSHPRYFTE 250
    RLIRACAELP KVCEYFHIPF QSGDNDVLKA MARGYTVERY LRIVEQIRDI 300
    IPDAAISADA IVAFPGETEE QFENTLKLVE QVGFDLVNTA AYSPRPGTPA 350
    ANAPNQLSEE VKQDRLQRLN HLVAQMAADR SQRYLGRTEE VLIEATNPRN 400
    PQQVMGRTRT NRLVFCDGTI AQLEGQLVPV RITETRAFSL TGQILSPVAA 450
    GC 452
    Length:452
    Mass (Da):50,656
    Last modified:February 1, 2005 - v1
    Checksum:i6EF09532ED2FA30C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008231 Genomic DNA. Translation: BAD79921.1.
    RefSeqiWP_011244041.1. NC_006576.1.
    YP_172441.1. NC_006576.1.

    Genome annotation databases

    EnsemblBacteriaiBAD79921; BAD79921; syc1731_c.
    GeneIDi3198429.
    KEGGisyc:syc1731_c.
    PATRICi32489474. VBISynElo117686_1977.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008231 Genomic DNA. Translation: BAD79921.1 .
    RefSeqi WP_011244041.1. NC_006576.1.
    YP_172441.1. NC_006576.1.

    3D structure databases

    ProteinModelPortali Q5N199.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269084.syc1731_c.

    Proteomic databases

    PRIDEi Q5N199.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD79921 ; BAD79921 ; syc1731_c .
    GeneIDi 3198429.
    KEGGi syc:syc1731_c.
    PATRICi 32489474. VBISynElo117686_1977.

    Phylogenomic databases

    eggNOGi COG0621.
    HOGENOMi HOG000224767.
    KOi K06168.
    OMAi IEVYKDT.
    OrthoDBi EOG6P5ZD8.

    Enzyme and pathway databases

    BioCyci SELO269084:GCDQ-1778-MONOMER.

    Family and domain databases

    Gene3Di 3.80.30.20. 1 hit.
    HAMAPi MF_01864. tRNA_metthiotr_MiaB.
    InterProi IPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view ]
    PANTHERi PTHR11918. PTHR11918. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
    PROSITEi PS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
      Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
      Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

    Entry informationi

    Entry nameiMIAB_SYNP6
    AccessioniPrimary (citable) accession number: Q5N199
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3