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Q5N006 (SYL_SYNP6) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:syc2174_d
OrganismSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) [Complete proteome] [HAMAP]
Taxonomic identifier269084 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length865 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 865865Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152103

Regions

Motif58 – 6811"HIGH" region HAMAP-Rule MF_00049
Motif629 – 6335"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5N006 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 707E35820B4B5539

FASTA86597,356
        10         20         30         40         50         60 
MQNGANSRSQ EYQGVSVDSR YDPQAIETKW QQSWAAAQLD RTPEADDRPK FYALSMFPYP 

        70         80         90        100        110        120 
SGNLHMGHVR NYTITDAIAR VKRRQGFRVL HAMGWDAFGL PAENAAIDRG VQPADWTYQN 

       130        140        150        160        170        180 
VAQMREQLKQ LGLSYDWDRE VTTCSPDYYR WTQWLFLQFF EAGLAYQKEA TVNWDPIDQT 

       190        200        210        220        230        240 
VLANEQVDSE GRSWRSGAKV ERRQLKQWFL KITDYAEELL QDLDQLTGWP ERVRLMQANW 

       250        260        270        280        290        300 
IGKSTGAYLE FPIVNSSDRV KVFTTRPDTV YGVSYVVLAP EHPLVTQVTT PEQQTAVAAF 

       310        320        330        340        350        360 
AAEVSQTSEL ERTAEDRPKR GVPTGGFVTN PFTGQAVPIW IADYVLVEYG TGAVMGVPAH 

       370        380        390        400        410        420 
DSRDFAFAQR YGLPVQPVIQ PTEGAIAEPW PAPFTEAGVM VNSGQFDGLS STEAKAKIIA 

       430        440        450        460        470        480 
FAEEQGWGQA HVQYRLRDWL ISRQRYWGCP IPIVHCPDCG PVAAADLPVQ LPDSVQFSGR 

       490        500        510        520        530        540 
GPSPLAQLED WVTTTCPSCG KPARRETDTM DTFMCSSWYY LRYSDASNPE IAFTKDKVND 

       550        560        570        580        590        600 
WLPVDQYVGG IEHAILHLLY SRFFTKVLRD RGLLSFDEPF KRLLTQGMVQ GLTYKNPKTG 

       610        620        630        640        650        660 
KYVPSDRISD PSQPVDPDTG DRLEVFFEKM SKSKYNGVDP ARVLDRYGAD TARMFILFKA 

       670        680        690        700        710        720 
PPEKDLEWDD ADVEGQFRFL NRVWRLVQTA SQVEATTAAD DKAEKDLRRA VHTAIQAFTE 

       730        740        750        760        770        780 
DLEEDYQLNT AIAELMKLTN ALNDAPMPGS PAYLKGVQTL VLLLAPFAPH IAEELWQQLG 

       790        800        810        820        830        840 
GERSVHLEGW PVLDESALIV DEIPLVIQIM GKTRGTITVP ASADRDQLQQ LAKNSEIAQR 

       850        860 
WLDGQTIRKV IVVPGKLVNF VIASP 

« Hide

References

[1]"Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008231 Genomic DNA. Translation: BAD80364.1.
RefSeqYP_172884.1. NC_006576.1.

3D structure databases

ProteinModelPortalQ5N006.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269084.syc2174_d.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD80364; BAD80364; syc2174_d.
GeneID3199910.
KEGGsyc:syc2174_d.
PATRIC32490494. VBISynElo117686_2477.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSELO269084:GCDQ-2230-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SYNP6
AccessionPrimary (citable) accession number: Q5N006
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries