Q5MZY3 (LEUC_SYNP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase large subunit
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase

Gene names

Name:	leuC
Ordered Locus Names:	syc2197_d

Organism

Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) [Complete proteome] [HAMAP]

Taxonomic identifier

269084 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus › Synechococcus elongatus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026
Subunit structure	Heterodimer of LeuC and LeuD By similarity.
Sequence similarities	Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: HAMAP 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	3-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026		PRO_0000076829
Sites
	Metal binding	347	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	407	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	410	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5MZY3 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 9D19B242070323BB	FASTA	468	50,343
	10 20 30 40 50 60 MSRGTLFDKV WDLHTVATLP SGQTQLFIGL HLIHEVTSPQ AFSMLRDRGL TVKFPGRTVA 70 80 90 100 110 120 TVDHIVPTEN QARPFADSLA EEMIVTLERN CRENGIRFYN IGSGSQGIVH VIAPEQGLTQ 130 140 150 160 170 180 PGMTIACGDS HTSTHGAFGA IAFGIGTSQV RDVLASQTLA LSKLKVRKIE VNGELQPGVY 190 200 210 220 230 240 AKDVILHIIR KLGVKGGVGY AYEFAGSTFA AMSMEERMTV CNMAIEGGAR CGYVNPDQIT 250 260 270 280 290 300 YDYLQGREFA PQGEAWDRAI AWWESLRSEA DAEYDDVVVF DAAEIAPTVT WGITPGQGIG 310 320 330 340 350 360 ITETIPTPDS LLDEDRAVAA EAYSYMDLEP GAPLQGTKVD VCFIGSCTNG RLSDLREAAK 370 380 390 400 410 420 VAQGRKVAAG IKAFVVPGSE RVKQQAEAEG LDQIFTAAGF EWRQAGCSMC LAMNPDKLEG 430 440 450 460 RQISASSSNR NFKGRQGSAS GRTLLMSPAM VAAAAIAGEV TDVRNWLN

References

[1]

"Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization." Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M. Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008231 Genomic DNA. Translation: BAD80387.1.
RefSeq	YP_172907.1. NC_006576.1.
3D structure databases
ProteinModelPortal	Q5MZY3.
ModBase	Search...
Protein-protein interaction databases
STRING	269084.syc2197_d.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD80387; BAD80387; syc2197_d.
GeneID	3199884.
KEGG	syc:syc2197_d.
PATRIC	32490540. VBISynElo117686_2500.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0065.
HOGENOM	HOG000226972.
KO	K01703.
OMA	DIRQGIV.
ProtClustDB	PRK05478.
Enzyme and pathway databases
UniPathway	UPA00048; UER00071.
Family and domain databases
Gene3D	3.30.499.10. 2 hits. 3.40.1060.10. 1 hit.
HAMAP	MF_01026. LeuC_type1.
InterPro	IPR004430. 3-IsopropMal_deHydase_lsu. IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF53732. Aconitase_N. 1 hit.
TIGRFAMs	TIGR00170. leuC. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEUC_SYNP6

Accession

Primary (citable) accession number: Q5MZY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program