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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi407 – 4071Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi410 – 4101Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciSELO269084:GCDQ-2253-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:syc2197_d
OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Taxonomic identifieri269084 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001175: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4684683-isopropylmalate dehydratase large subunitPRO_0000076829Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi269084.syc2197_d.

Structurei

3D structure databases

ProteinModelPortaliQ5MZY3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiDKVWDLH.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5MZY3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRGTLFDKV WDLHTVATLP SGQTQLFIGL HLIHEVTSPQ AFSMLRDRGL
60 70 80 90 100
TVKFPGRTVA TVDHIVPTEN QARPFADSLA EEMIVTLERN CRENGIRFYN
110 120 130 140 150
IGSGSQGIVH VIAPEQGLTQ PGMTIACGDS HTSTHGAFGA IAFGIGTSQV
160 170 180 190 200
RDVLASQTLA LSKLKVRKIE VNGELQPGVY AKDVILHIIR KLGVKGGVGY
210 220 230 240 250
AYEFAGSTFA AMSMEERMTV CNMAIEGGAR CGYVNPDQIT YDYLQGREFA
260 270 280 290 300
PQGEAWDRAI AWWESLRSEA DAEYDDVVVF DAAEIAPTVT WGITPGQGIG
310 320 330 340 350
ITETIPTPDS LLDEDRAVAA EAYSYMDLEP GAPLQGTKVD VCFIGSCTNG
360 370 380 390 400
RLSDLREAAK VAQGRKVAAG IKAFVVPGSE RVKQQAEAEG LDQIFTAAGF
410 420 430 440 450
EWRQAGCSMC LAMNPDKLEG RQISASSSNR NFKGRQGSAS GRTLLMSPAM
460
VAAAAIAGEV TDVRNWLN
Length:468
Mass (Da):50,343
Last modified:February 1, 2005 - v1
Checksum:i9D19B242070323BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA. Translation: BAD80387.1.
RefSeqiWP_011244507.1. NC_006576.1.
YP_172907.1. NC_006576.1.

Genome annotation databases

EnsemblBacteriaiBAD80387; BAD80387; syc2197_d.
GeneIDi3199884.
KEGGisyc:syc2197_d.
PATRICi32490540. VBISynElo117686_2500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008231 Genomic DNA. Translation: BAD80387.1.
RefSeqiWP_011244507.1. NC_006576.1.
YP_172907.1. NC_006576.1.

3D structure databases

ProteinModelPortaliQ5MZY3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269084.syc2197_d.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD80387; BAD80387; syc2197_d.
GeneIDi3199884.
KEGGisyc:syc2197_d.
PATRICi32490540. VBISynElo117686_2500.

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiDKVWDLH.
OrthoDBiEOG600DP5.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.
BioCyciSELO269084:GCDQ-2253-MONOMER.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
    Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
    Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Entry informationi

Entry nameiLEUC_SYNP6
AccessioniPrimary (citable) accession number: Q5MZY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: February 1, 2005
Last modified: January 7, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.