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Q5MZY3 (LEUC_SYNP6) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase large subunit

EC=4.2.1.33
Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase
Gene names
Name:leuC
Ordered Locus Names:syc2197_d
OrganismSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) [Complete proteome] [HAMAP]
Taxonomic identifier269084 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01026

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01026

Sequence similarities

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4684683-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026
PRO_0000076829

Sites

Metal binding3471Iron-sulfur (4Fe-4S) By similarity
Metal binding4071Iron-sulfur (4Fe-4S) By similarity
Metal binding4101Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5MZY3 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 9D19B242070323BB

FASTA46850,343
        10         20         30         40         50         60 
MSRGTLFDKV WDLHTVATLP SGQTQLFIGL HLIHEVTSPQ AFSMLRDRGL TVKFPGRTVA 

        70         80         90        100        110        120 
TVDHIVPTEN QARPFADSLA EEMIVTLERN CRENGIRFYN IGSGSQGIVH VIAPEQGLTQ 

       130        140        150        160        170        180 
PGMTIACGDS HTSTHGAFGA IAFGIGTSQV RDVLASQTLA LSKLKVRKIE VNGELQPGVY 

       190        200        210        220        230        240 
AKDVILHIIR KLGVKGGVGY AYEFAGSTFA AMSMEERMTV CNMAIEGGAR CGYVNPDQIT 

       250        260        270        280        290        300 
YDYLQGREFA PQGEAWDRAI AWWESLRSEA DAEYDDVVVF DAAEIAPTVT WGITPGQGIG 

       310        320        330        340        350        360 
ITETIPTPDS LLDEDRAVAA EAYSYMDLEP GAPLQGTKVD VCFIGSCTNG RLSDLREAAK 

       370        380        390        400        410        420 
VAQGRKVAAG IKAFVVPGSE RVKQQAEAEG LDQIFTAAGF EWRQAGCSMC LAMNPDKLEG 

       430        440        450        460 
RQISASSSNR NFKGRQGSAS GRTLLMSPAM VAAAAIAGEV TDVRNWLN 

« Hide

References

[1]"Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization."
Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M.
Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008231 Genomic DNA. Translation: BAD80387.1.
RefSeqYP_172907.1. NC_006576.1.

3D structure databases

ProteinModelPortalQ5MZY3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269084.syc2197_d.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD80387; BAD80387; syc2197_d.
GeneID3199884.
KEGGsyc:syc2197_d.
PATRIC32490540. VBISynElo117686_2500.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0065.
HOGENOMHOG000226972.
KOK01703.
OMADKVWDLH.
OrthoDBEOG600DP5.
ProtClustDBPRK05478.

Enzyme and pathway databases

BioCycSELO269084:GCDQ-2253-MONOMER.
UniPathwayUPA00048; UER00071.

Family and domain databases

Gene3D3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPMF_01026. LeuC_type1.
InterProIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PfamPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. SSF53732. 1 hit.
TIGRFAMsTIGR00170. leuC. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEUC_SYNP6
AccessionPrimary (citable) accession number: Q5MZY3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: February 1, 2005
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways