Q5MZA4 (SYC_SYNP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	syc2426_d

Organism

Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) [Complete proteome] [HAMAP]

Taxonomic identifier

269084 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus › Synechococcus elongatus

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_0000159503
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	270 – 274	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	213	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Metal binding	242	1	Zinc By similarity
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5MZA4 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 76228D430A61E0EB	FASTA	478	54,360
	10 20 30 40 50 60 MTLSLYNTLT RQKQRFEPLQ AGSVSLYCCG VTVYDYCHLG HARSYIVWDT LRRYLLWLGY 70 80 90 100 110 120 RVRYVQNFTD IDDKILRRSR QEGTTMQAIA DRYTQAYFED MARLNILEAD DYPRATHTLD 130 140 150 160 170 180 GIQRLIAELE DKGFAYAADG DVYFSVRRFQ DYGKLSGRKL EDLKAGASGR VESAEESLKH 190 200 210 220 230 240 DPFDFALWKA AKPEEPAWDS PWGPGRPGWH IECSAMVRDR LGDSIDIHVG GADLVFPHHE 250 260 270 280 290 300 NEIAQSEAVT GHPLARYWLH NGMVNVGGEK MSKSLGNFTT IRQLLDEGGI SPMVLRLFVL 310 320 330 340 350 360 QANYRKPIDF TDEALQACQN GWETLQEGLH FGEHWGDRLG WTESVTVDPD LSDRFRMAMD 370 380 390 400 410 420 DDLNTPAALA VLFELAKELR RQQNLLIHEG HLDGDAQQLH QHWVTLVQLA GVLGLEAEPE 430 440 450 460 470 LAETNELDEA AIEDWIAKRH AARQAKDFAE ADRIRHYLAD LGITLIDQAG GITRWSRT

References

[1]

"Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization." Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M. Photosyn. Res. 93:55-67(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008231 Genomic DNA. Translation: BAD80616.1.
RefSeq	YP_173136.1. NC_006576.1.
3D structure databases
ProteinModelPortal	Q5MZA4.
ModBase	Search...
Protein-protein interaction databases
STRING	269084.syc2426_d.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD80616; BAD80616; syc2426_d.
GeneID	3199160.
KEGG	syc:syc2426_d.
PATRIC	32491058. VBISynElo117686_2759.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HOG000245250.
KO	K01883.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth.
InterPro	IPR015803. Cys-tRNA-ligase. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_SYNP6

Accession

Primary (citable) accession number: Q5MZA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program