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Protein

Catalase-peroxidase

Gene

katG

Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation2 Publications

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.

Enzyme regulationi

Inhibited by cyanide.1 Publication

Kineticsi

  1. KM=4.3 mM for H2O2 for the catalase reaction1 Publication

    pH dependencei

    Optimum pH is 6.5-7.5 for both the catalase and the peroxidase reaction.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei91Transition state stabilizerUniRule annotation1
    Active sitei95Proton acceptorUniRule annotation1
    Metal bindingi263Iron (heme axial ligand)UniRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Biological processi

    Hydrogen peroxide

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Protein family/group databases

    PeroxiBasei3578. SeCP01_PCC6301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
    Short name:
    CPUniRule annotation
    Alternative name(s):
    Peroxidase/catalaseUniRule annotation
    Gene namesi
    Name:katGUniRule annotation
    Ordered Locus Names:syc2431_d
    OrganismiSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
    Taxonomic identifieri269084 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
    Proteomesi
    • UP000001175 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003549411 – 720Catalase-peroxidaseAdd BLAST720

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki94 ↔ 222Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-248)UniRule annotation
    Cross-linki222 ↔ 248Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-94)UniRule annotation

    Post-translational modificationi

    The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

    Proteomic databases

    PRIDEiQ5MZ99.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    STRINGi269084.syc2431_d.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5MZ99.
    SMRiQ5MZ99.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0376. LUCA.
    HOGENOMiHOG000218110.
    KOiK03782.
    OMAiIAEVYAC.
    OrthoDBiPOG091H05R1.

    Family and domain databases

    HAMAPiMF_01961. Catal_peroxid. 1 hit.
    InterProiIPR000763. Catalase_peroxidase.
    IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR019794. Peroxidases_AS.
    [Graphical view]
    PfamiPF00141. peroxidase. 2 hits.
    [Graphical view]
    PRINTSiPR00460. BPEROXIDASE.
    PR00458. PEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 2 hits.
    TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
    PROSITEiPS00436. PEROXIDASE_2. 1 hit.
    PS50873. PEROXIDASE_4. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5MZ99-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTATQGKCPV MHGGATTVNI STAEWWPKAL NLDILSQHDR KTNPMGPDFN
    60 70 80 90 100
    YQEEVKKLDV AALKQDLQAL MTDSQDWWPA DWGHYGGLMI RLTWHAAGTY
    110 120 130 140 150
    RIADGRGGAG TGNQRFAPLN SWPDNTNLDK ARRLLWPIKQ KYGNKLSWAD
    160 170 180 190 200
    LIAYAGTIAY ESMGLKTFGF AFGREDIWHP EKDIYWGPEK EWVPPSTNPN
    210 220 230 240 250
    SRYTGDRELE NPLAAVTMGL IYVNPEGVDG NPDPLKTAHD VRVTFARMAM
    260 270 280 290 300
    NDEETVALTA GGHTVGKCHG NGNAALLGPE PEGADVEDQG LGWINKTQSG
    310 320 330 340 350
    IGRNAVTSGL EGAWTPHPTQ WDNGYFRMLL NYDWELKKSP AGAWQWEPIN
    360 370 380 390 400
    PREEDLPVDV EDPSIRRNLV MTDADMAMKM DPEYRKISER FYQDPAYFAD
    410 420 430 440 450
    VFARAWFKLT HRDMGPKARY IGPDVPQEDL IWQDPIPAGN RNYDVQAVKD
    460 470 480 490 500
    RIAASGLSIS ELVSTAWDSA RTYRNSDKRG GANGARIRLA PQKDWEGNEP
    510 520 530 540 550
    DRLAKVLAVL EGIAAATGAS VADVIVLAGN VGVEQAARAA GVEIVLPFAP
    560 570 580 590 600
    GRGDATAEQT DTESFAVLEP IHDGYRNWLK QDYAATPEEL LLDRTQLLGL
    610 620 630 640 650
    TAPEMTVLIG GLRVLGTNHG GTKHGVFTDR EGVLTNDFFV NLTDMNYLWK
    660 670 680 690 700
    PAGKNLYEIC DRKTNQVKWT ATRVDLVFGS NSILRAYSEL YAQDDNKEKF
    710 720
    VRDFVAAWTK VMNADRFDLD
    Length:720
    Mass (Da):80,078
    Last modified:February 1, 2005 - v1
    Checksum:i93A4C2E9990F1A2F
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti5Q → R in AAF05841 (PubMed:10863004).Curated1
    Sequence conflicti358V → I in AAF05841 (PubMed:10863004).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF197161 Genomic DNA. Translation: AAF05841.1.
    AP008231 Genomic DNA. Translation: BAD80621.1.
    RefSeqiWP_011244741.1. NC_006576.1.

    Genome annotation databases

    EnsemblBacteriaiBAD80621; BAD80621; syc2431_d.
    KEGGisyc:syc2431_d.
    PATRICi32491070. VBISynElo117686_2765.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF197161 Genomic DNA. Translation: AAF05841.1.
    AP008231 Genomic DNA. Translation: BAD80621.1.
    RefSeqiWP_011244741.1. NC_006576.1.

    3D structure databases

    ProteinModelPortaliQ5MZ99.
    SMRiQ5MZ99.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi269084.syc2431_d.

    Protein family/group databases

    PeroxiBasei3578. SeCP01_PCC6301.

    Proteomic databases

    PRIDEiQ5MZ99.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAD80621; BAD80621; syc2431_d.
    KEGGisyc:syc2431_d.
    PATRICi32491070. VBISynElo117686_2765.

    Phylogenomic databases

    eggNOGiCOG0376. LUCA.
    HOGENOMiHOG000218110.
    KOiK03782.
    OMAiIAEVYAC.
    OrthoDBiPOG091H05R1.

    Family and domain databases

    HAMAPiMF_01961. Catal_peroxid. 1 hit.
    InterProiIPR000763. Catalase_peroxidase.
    IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR019794. Peroxidases_AS.
    [Graphical view]
    PfamiPF00141. peroxidase. 2 hits.
    [Graphical view]
    PRINTSiPR00460. BPEROXIDASE.
    PR00458. PEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 2 hits.
    TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
    PROSITEiPS00436. PEROXIDASE_2. 1 hit.
    PS50873. PEROXIDASE_4. 2 hits.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiKATG_SYNP6
    AccessioniPrimary (citable) accession number: Q5MZ99
    Secondary accession number(s): Q9R6S9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: February 1, 2005
    Last modified: November 2, 2016
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.