Q5MZ99 (KATG_SYNP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	syc2431_d

Organism

Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) [Complete proteome] [HAMAP]

Taxonomic identifier

269084 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Ref.1 Ref.3
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer. Ref.1
Enzyme regulation	Inhibited by cyanide. Ref.1
Subunit structure	Homodimer. Ref.1 Ref.3
Subcellular location	Cytoplasm › cytosol Ref.3.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.
Biophysicochemical properties	Kinetic parameters: KM=4.3 mM for H2O2 for the catalase reaction Ref.3 pH dependence: Optimum pH is 6.5-7.5 for both the catalase and the peroxidase reaction.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Catalase-peroxidase HAMAP MF_01961		PRO_0000354941
Sites
	Active site	95	1	Proton acceptor By similarity
	Metal binding	263	1	Iron (heme axial ligand) By similarity
	Site	91	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	94 ↔ 222		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-248) By similarity
	Cross-link	222 ↔ 248		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-94) By similarity
Experimental info
	Sequence conflict	5	1	Q → R in AAF05841. Ref.1
	Sequence conflict	358	1	V → I in AAF05841. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5MZ99 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 93A4C2E9990F1A2F	FASTA	720	80,078
	10 20 30 40 50 60 MTATQGKCPV MHGGATTVNI STAEWWPKAL NLDILSQHDR KTNPMGPDFN YQEEVKKLDV 70 80 90 100 110 120 AALKQDLQAL MTDSQDWWPA DWGHYGGLMI RLTWHAAGTY RIADGRGGAG TGNQRFAPLN 130 140 150 160 170 180 SWPDNTNLDK ARRLLWPIKQ KYGNKLSWAD LIAYAGTIAY ESMGLKTFGF AFGREDIWHP 190 200 210 220 230 240 EKDIYWGPEK EWVPPSTNPN SRYTGDRELE NPLAAVTMGL IYVNPEGVDG NPDPLKTAHD 250 260 270 280 290 300 VRVTFARMAM NDEETVALTA GGHTVGKCHG NGNAALLGPE PEGADVEDQG LGWINKTQSG 310 320 330 340 350 360 IGRNAVTSGL EGAWTPHPTQ WDNGYFRMLL NYDWELKKSP AGAWQWEPIN PREEDLPVDV 370 380 390 400 410 420 EDPSIRRNLV MTDADMAMKM DPEYRKISER FYQDPAYFAD VFARAWFKLT HRDMGPKARY 430 440 450 460 470 480 IGPDVPQEDL IWQDPIPAGN RNYDVQAVKD RIAASGLSIS ELVSTAWDSA RTYRNSDKRG 490 500 510 520 530 540 GANGARIRLA PQKDWEGNEP DRLAKVLAVL EGIAAATGAS VADVIVLAGN VGVEQAARAA 550 560 570 580 590 600 GVEIVLPFAP GRGDATAEQT DTESFAVLEP IHDGYRNWLK QDYAATPEEL LLDRTQLLGL 610 620 630 640 650 660 TAPEMTVLIG GLRVLGTNHG GTKHGVFTDR EGVLTNDFFV NLTDMNYLWK PAGKNLYEIC 670 680 690 700 710 720 DRKTNQVKWT ATRVDLVFGS NSILRAYSEL YAQDDNKEKF VRDFVAAWTK VMNADRFDLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis, overexpression in Escherichia coli and kinetic characterization of Anacystis nidulans catalase-peroxidase." Engleder M., Regelsberger G., Jakopitsch C., Furtmueller P.G., Rueker F., Peschek G.A., Obinger C. Biochimie 82:211-219(2000) [PubMed: 10863004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, HEME-BINDING, ENZYME REGULATION.
[2]	"Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization." Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M., Kanehisa M., Omata T., Sugiura M., Sugita M. Photosyn. Res. 93:55-67(2007) [PubMed: 17211581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1.
[3]	"Purification and characterization of a homodimeric catalase-peroxidase from the cyanobacterium Anacystis nidulans." Obinger C., Regelsberger G., Strasser G., Burner U., Peschek G.A. Biochem. Biophys. Res. Commun. 235:545-552(1997) [PubMed: 9207193] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF197161 Genomic DNA. Translation: AAF05841.1. AP008231 Genomic DNA. Translation: BAD80621.1.
RefSeq	YP_173141.1. NC_006576.1.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ProteinModelPortal	Q5MZ99.
SMR	Q5MZ99. Positions 21-720.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5MZ99.
Protein family/group databases
PeroxiBase	3578. SeCP01_PCC6301.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3199022.
GenomeReviews	Gene locus syc2431_d in contig AP008231_GR.
KEGG	syc:syc2431_d.
PATRIC	32491070. VBISynElo117686_2765.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	IRLTWHA.
PhylomeDB	Q5MZ99.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	SELO269084:SYC2431_D-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SYNP6

Accession

Primary (citable) accession number: Q5MZ99
Secondary accession number(s): Q9R6S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program