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Q5MY95

- ENTP8_HUMAN

UniProt

Q5MY95 - ENTP8_HUMAN

Protein

Ectonucleoside triphosphate diphosphohydrolase 8

Gene

ENTPD8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP.2 Publications

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.2 Publications

    Cofactori

    Ca2+ or Mg2+. Has lower efficiency with Mg2+ By similarity.By similarity

    Enzyme regulationi

    Not inhibited by ARL 67156.1 Publication

    Kineticsi

    1. KM=81 µM for ATP1 Publication
    2. KM=137 µM for ADP1 Publication
    3. KM=480 µM for UTP1 Publication
    4. KM=241 µM for UDP1 Publication

    Vmax=790 nmol/min/mg enzyme with ATP as substrate1 Publication

    Vmax=163 nmol/min/mg enzyme with ADP as substrate1 Publication

    Vmax=1100 nmol/min/mg enzyme with UTP as substrate1 Publication

    Vmax=110 nmol/min/mg enzyme with UDP as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei168 – 1681Proton acceptorBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. nucleoside-diphosphatase activity Source: Ensembl
    4. nucleoside-triphosphatase activity Source: Ensembl

    GO - Biological processi

    1. nucleoside diphosphate biosynthetic process Source: Ensembl
    2. nucleoside monophosphate biosynthetic process Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKQ5MY95.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)
    Short name:
    E-NTPDase 8
    Short name:
    NTPDase 8
    Short name:
    NTPDase8
    Gene namesi
    Name:ENTPD8
    ORF Names:UNQ2492/PRO5779
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:24860. ENTPD8.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671906.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495Ectonucleoside triphosphate diphosphohydrolase 8PRO_0000306882Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi67 – 671N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi78 ↔ 102By similarity
    Disulfide bondi246 ↔ 292By similarity
    Glycosylationi304 – 3041N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi329 ↔ 335By similarity
    Glycosylationi363 – 3631N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi381 ↔ 403By similarity

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ5MY95.
    PRIDEiQ5MY95.

    PTM databases

    PhosphoSiteiQ5MY95.

    Expressioni

    Gene expression databases

    ArrayExpressiQ5MY95.
    BgeeiQ5MY95.
    CleanExiHS_ENTPD8.
    GenevestigatoriQ5MY95.

    Organism-specific databases

    HPAiHPA021509.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000360561.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5MY95.
    SMRiQ5MY95. Positions 41-458.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88CytoplasmicSequence Analysis
    Topological domaini30 – 471442ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini493 – 4953CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei472 – 49221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The transmembranous domains are involved in regulation of enzyme activity.1 Publication

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5371.
    HOGENOMiHOG000059572.
    HOVERGENiHBG018982.
    InParanoidiQ5MY95.
    KOiK01510.
    OMAiVDFWGAE.
    OrthoDBiEOG754HPX.
    PhylomeDBiQ5MY95.
    TreeFamiTF332859.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]
    PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5MY95-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGLSRKEQVF LALLGASGVS GLTALILLLV EATSVLLPTD IKFGIVFDAG    50
    SSHTSLFLYQ WLANKENGTG VVSQALACQV EGPGISSYTS NAAQAGESLQ 100
    GCLEEALVLI PEAQHRKTPT FLGATAGMRL LSRKNSSQAR DIFAAVTQVL 150
    GRSPVDFWGA ELLAGQAEGA FGWITVNYGL GTLVKYSFTG EWIQPPEEML 200
    VGALDMGGAS TQITFVPGGP ILDKSTQADF RLYGSDYSVY THSYLCFGRD 250
    QMLSRLLVGL VQSRPAALLR HPCYLSGYQT TLALGPLYES PCVHATPPLS 300
    LPQNLTVEGT GNPGACVSAI RELFNFSSCQ GQEDCAFDGV YQPPLRGQFY 350
    AFSNFYYTFH FLNLTSRQPL STVNATIWEF CQRPWKLVEA SYPGQDRWLR 400
    DYCASGLYIL TLLHEGYGFS EETWPSLEFR KQAGGVDIGW TLGYMLNLTG 450
    MIPADAPAQW RAESYGVWVA KVVFMVLALV AVVGAALVQL FWLQD 495
    Length:495
    Mass (Da):53,904
    Last modified:October 2, 2007 - v2
    Checksum:i030200E15F662D82
    GO
    Isoform 2 (identifier: Q5MY95-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         351-387: Missing.

    Show »
    Length:458
    Mass (Da):49,393
    Checksum:iC2BEDCB65064B6B3
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti62 – 621L → P.4 Publications
    Corresponds to variant rs6606582 [ dbSNP | Ensembl ].
    VAR_035339
    Natural varianti428 – 4281E → K.
    Corresponds to variant rs61491031 [ dbSNP | Ensembl ].
    VAR_061385

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei351 – 38737Missing in isoform 2. 2 PublicationsVSP_028559Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY903953 Transcribed RNA. Translation: AAW83515.1.
    AY903954 mRNA. Translation: AAW83516.1.
    AY430414 mRNA. Translation: AAR04374.1.
    AY359088 mRNA. Translation: AAQ89446.1.
    BX322799 Genomic DNA. Translation: CAM24777.1.
    BC141810 mRNA. Translation: AAI41811.1.
    CCDSiCCDS43913.1. [Q5MY95-1]
    CCDS7043.1. [Q5MY95-2]
    RefSeqiNP_001028285.1. NM_001033113.1. [Q5MY95-1]
    NP_940987.2. NM_198585.2. [Q5MY95-2]
    UniGeneiHs.512562.

    Genome annotation databases

    EnsembliENST00000344119; ENSP00000344089; ENSG00000188833. [Q5MY95-2]
    ENST00000371506; ENSP00000360561; ENSG00000188833. [Q5MY95-1]
    ENST00000472938; ENSP00000420531; ENSG00000188833. [Q5MY95-1]
    GeneIDi377841.
    KEGGihsa:377841.
    UCSCiuc004cmw.3. human. [Q5MY95-1]
    uc004cmx.3. human. [Q5MY95-2]

    Polymorphism databases

    DMDMi158705943.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY903953 Transcribed RNA. Translation: AAW83515.1 .
    AY903954 mRNA. Translation: AAW83516.1 .
    AY430414 mRNA. Translation: AAR04374.1 .
    AY359088 mRNA. Translation: AAQ89446.1 .
    BX322799 Genomic DNA. Translation: CAM24777.1 .
    BC141810 mRNA. Translation: AAI41811.1 .
    CCDSi CCDS43913.1. [Q5MY95-1 ]
    CCDS7043.1. [Q5MY95-2 ]
    RefSeqi NP_001028285.1. NM_001033113.1. [Q5MY95-1 ]
    NP_940987.2. NM_198585.2. [Q5MY95-2 ]
    UniGenei Hs.512562.

    3D structure databases

    ProteinModelPortali Q5MY95.
    SMRi Q5MY95. Positions 41-458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000360561.

    Chemistry

    BindingDBi Q5MY95.
    ChEMBLi CHEMBL5338.

    PTM databases

    PhosphoSitei Q5MY95.

    Polymorphism databases

    DMDMi 158705943.

    Proteomic databases

    PaxDbi Q5MY95.
    PRIDEi Q5MY95.

    Protocols and materials databases

    DNASUi 377841.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000344119 ; ENSP00000344089 ; ENSG00000188833 . [Q5MY95-2 ]
    ENST00000371506 ; ENSP00000360561 ; ENSG00000188833 . [Q5MY95-1 ]
    ENST00000472938 ; ENSP00000420531 ; ENSG00000188833 . [Q5MY95-1 ]
    GeneIDi 377841.
    KEGGi hsa:377841.
    UCSCi uc004cmw.3. human. [Q5MY95-1 ]
    uc004cmx.3. human. [Q5MY95-2 ]

    Organism-specific databases

    CTDi 377841.
    GeneCardsi GC09M140328.
    HGNCi HGNC:24860. ENTPD8.
    HPAi HPA021509.
    neXtProti NX_Q5MY95.
    PharmGKBi PA142671906.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5371.
    HOGENOMi HOG000059572.
    HOVERGENi HBG018982.
    InParanoidi Q5MY95.
    KOi K01510.
    OMAi VDFWGAE.
    OrthoDBi EOG754HPX.
    PhylomeDBi Q5MY95.
    TreeFami TF332859.

    Enzyme and pathway databases

    SABIO-RK Q5MY95.

    Miscellaneous databases

    GenomeRNAii 377841.
    NextBioi 100687.
    PROi Q5MY95.

    Gene expression databases

    ArrayExpressi Q5MY95.
    Bgeei Q5MY95.
    CleanExi HS_ENTPD8.
    Genevestigatori Q5MY95.

    Family and domain databases

    InterProi IPR000407. GDA1_CD39_NTPase.
    [Graphical view ]
    PANTHERi PTHR11782. PTHR11782. 1 hit.
    Pfami PF01150. GDA1_CD39. 1 hit.
    [Graphical view ]
    PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of expressed human ecto-nucleoside triphosphate diphosphohydrolase 8 (E-NTPDase 8) and its soluble extracellular domain."
      Knowles A.F., Li C.
      Biochemistry 45:7323-7333(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME ACTIVITY, DOMAIN, GLYCOSYLATION, VARIANT PRO-62.
    2. "Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8."
      Fausther M., Lecka J., Kukulski F., Levesque S.A., Pelletier J., Zimmermann H., Dranoff J.A., Sevigny J.
      Am. J. Physiol. 292:G785-G795(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, VARIANT PRO-62.
      Tissue: Liver.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-62.
    4. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-62.
    6. "Specificity of the ecto-ATPase inhibitor ARL 67156 on human and mouse ectonucleotidases."
      Levesque S.A., Lavoie E.G., Lecka J., Bigonnesse F., Sevigny J.
      Br. J. Pharmacol. 152:141-150(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.

    Entry informationi

    Entry nameiENTP8_HUMAN
    AccessioniPrimary (citable) accession number: Q5MY95
    Secondary accession number(s): A2BG17, Q6UVZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3