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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Human coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.UniRule annotation

Miscellaneous

Isolate N1 belongs to genotype A.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei34NucleophileUniRule annotation1
Active sitei296Charge relay systemUniRule annotation1
Active sitei299Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esteraseUniRule annotation (EC:3.1.1.53UniRule annotation)
Short name:
HE proteinUniRule annotation
Alternative name(s):
E3 glycoproteinUniRule annotation
Gene namesi
Name:HEUniRule annotation
ORF Names:2
OrganismiHuman coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Taxonomic identifieri443239 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008170 Componenti: Genome

Subcellular locationi

  • Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host cell membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini12 – 359Virion surfaceUniRule annotationAdd BLAST348
Transmembranei360 – 380HelicalUniRule annotationAdd BLAST21
Topological domaini381 – 386IntravirionUniRule annotation6

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 11UniRule annotationAdd BLAST11
ChainiPRO_000029776112 – 386Hemagglutinin-esteraseUniRule annotationAdd BLAST375

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 59UniRule annotation
Glycosylationi83N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi107 ↔ 154UniRule annotation
Glycosylationi110N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi145N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi168N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi180 ↔ 246UniRule annotation
Disulfide bondi188 ↔ 219UniRule annotation
Disulfide bondi277 ↔ 282UniRule annotation
Glycosylationi286N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi317 ↔ 341UniRule annotation
Glycosylationi328N-linked (GlcNAc...) asparagine; by hostUniRule annotation1

Post-translational modificationi

N-glycosylated in the host RER.UniRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5MQD1
SMRiQ5MQD1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 121Esterase domain 1UniRule annotationAdd BLAST121
Regioni122 – 236Receptor bindingUniRule annotationAdd BLAST115
Regioni237 – 349Esterase domain 2UniRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.UniRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK19253
OrthoDBiVOG0900009Y

Family and domain databases

HAMAPiMF_04207 BETA_CORONA_HE, 1 hit
InterProiView protein in InterPro
IPR008980 Capsid_hemagglutn
IPR007142 Hemagglutn-estrase_core
IPR003860 Hemagglutn-estrase_hemagglutn
PfamiView protein in Pfam
PF03996 Hema_esterase, 1 hit
PF02710 Hema_HEFG, 1 hit
SUPFAMiSSF49818 SSF49818, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5MQD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIIFLFFYF CYGFNEPLNV VSHLNHDWFL FGDSRSDCNH INNLKIKNFD
60 70 80 90 100
YLDIHPSLCN NGKISSSAGD SIFKSFHFTR FYNYTGEGDQ IIFYEGVNFN
110 120 130 140 150
PYHRFKCFPN GSNDVWLLNK VRFYRALYSN MAFFRYLTFV DIPYNVSLSK
160 170 180 190 200
FNSCKSDILS LNNPIFINYS KEVYFTLLGC SLYLVPLCLF KSNFSQYYYN
210 220 230 240 250
IDTGSVYGFS NVVYPDLDCI YISLKPGSYK VSTTAPFLSL PTKALCFDKS
260 270 280 290 300
KQFVPVQVVD SRWNNERASD ISLSVACQLP YCYFRNSSAN YVGKYDINHG
310 320 330 340 350
DSGFISILSG LLYNVSCISY YGVFLYDNFT SIWPYYSFGR CPTSSIIKHP
360 370 380
ICVYDFLPII LQGILLCLAL LFVVFLLFLL YNDKSH
Length:386
Mass (Da):44,679
Last modified:February 1, 2005 - v1
Checksum:i8A880856BCDE8818
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY597011 Genomic RNA Translation: AAT98579.1
RefSeqiYP_173237.1, NC_006577.2

Genome annotation databases

GeneIDi3200425
KEGGivg:3200425

Similar proteinsi

Entry informationi

Entry nameiHEMA_CVHN1
AccessioniPrimary (citable) accession number: Q5MQD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: February 1, 2005
Last modified: June 20, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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