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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Human coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system (By similarity).By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341NucleophileBy similarity
Active sitei296 – 2961Charge relay systemBy similarity
Active sitei299 – 2991Charge relay systemBy similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2
OrganismiHuman coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Taxonomic identifieri443239 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008170 Componenti: Genome

Subcellular locationi

  1. Virion membrane Curated; Single-pass type I membrane protein Curated
  2. Host cell membrane Curated; Single-pass type I membrane protein Curated

  3. Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini14 – 358345Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei359 – 37921HelicalSequence AnalysisAdd
BLAST
Topological domaini380 – 3867IntravirionSequence Analysis

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1313Sequence AnalysisAdd
BLAST
Chaini14 – 386373Hemagglutinin-esterasePRO_0000297761Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 59By similarity
Glycosylationi83 – 831N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi107 ↔ 154By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi145 – 1451N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi168 – 1681N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi180 ↔ 246By similarity
Disulfide bondi188 ↔ 219By similarity
Glycosylationi193 – 1931N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi277 ↔ 282By similarity
Glycosylationi286 – 2861N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi314 – 3141N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi317 ↔ 341By similarity
Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5MQD1.
SMRiQ5MQD1. Positions 15-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 121121Esterase domain first partBy similarityAdd
BLAST
Regioni122 – 236115Receptor bindingBy similarityAdd
BLAST
Regioni237 – 349113Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5MQD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIIFLFFYF CYGFNEPLNV VSHLNHDWFL FGDSRSDCNH INNLKIKNFD
60 70 80 90 100
YLDIHPSLCN NGKISSSAGD SIFKSFHFTR FYNYTGEGDQ IIFYEGVNFN
110 120 130 140 150
PYHRFKCFPN GSNDVWLLNK VRFYRALYSN MAFFRYLTFV DIPYNVSLSK
160 170 180 190 200
FNSCKSDILS LNNPIFINYS KEVYFTLLGC SLYLVPLCLF KSNFSQYYYN
210 220 230 240 250
IDTGSVYGFS NVVYPDLDCI YISLKPGSYK VSTTAPFLSL PTKALCFDKS
260 270 280 290 300
KQFVPVQVVD SRWNNERASD ISLSVACQLP YCYFRNSSAN YVGKYDINHG
310 320 330 340 350
DSGFISILSG LLYNVSCISY YGVFLYDNFT SIWPYYSFGR CPTSSIIKHP
360 370 380
ICVYDFLPII LQGILLCLAL LFVVFLLFLL YNDKSH
Length:386
Mass (Da):44,679
Last modified:February 1, 2005 - v1
Checksum:i8A880856BCDE8818
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY597011 Genomic RNA. Translation: AAT98579.1.
RefSeqiYP_173237.1. NC_006577.2.

Genome annotation databases

GeneIDi3200425.
KEGGivg:3200425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY597011 Genomic RNA. Translation: AAT98579.1.
RefSeqiYP_173237.1. NC_006577.2.

3D structure databases

ProteinModelPortaliQ5MQD1.
SMRiQ5MQD1. Positions 15-345.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3200425.
KEGGivg:3200425.

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia."
    Woo P.C.Y., Lau S.K.P., Chu C.-M., Chan K.-H., Tsoi H.-W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J.J., Luk W.-K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M., Yuen K.-Y.
    J. Virol. 79:884-895(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_CVHN1
AccessioniPrimary (citable) accession number: Q5MQD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: February 1, 2005
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Isolate N1 belongs to genotype A.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.