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Q5MQD1

- HEMA_CVHN1

UniProt

Q5MQD1 - HEMA_CVHN1

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Human coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.By similarity

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei34 – 341NucleophileBy similarity
    Active sitei296 – 2961Charge relay systemBy similarity
    Active sitei299 – 2991Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    ORF Names:2
    OrganismiHuman coronavirus HKU1 (isolate N1) (HCoV-HKU1)
    Taxonomic identifieri443239 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008170: Genome

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1313Sequence AnalysisAdd
    BLAST
    Chaini14 – 386373Hemagglutinin-esterasePRO_0000297761Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi38 ↔ 59By similarity
    Glycosylationi83 – 831N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi107 ↔ 154By similarity
    Glycosylationi110 – 1101N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi168 – 1681N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi180 ↔ 246By similarity
    Disulfide bondi188 ↔ 219By similarity
    Glycosylationi193 – 1931N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi277 ↔ 282By similarity
    Glycosylationi286 – 2861N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi314 – 3141N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi317 ↔ 341By similarity
    Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    N-glycosylated in the RER.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5MQD1.
    SMRiQ5MQD1. Positions 15-345.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini14 – 358345Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini380 – 3867IntravirionSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei359 – 37921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 121121Esterase domain first partBy similarityAdd
    BLAST
    Regioni122 – 236115Receptor bindingBy similarityAdd
    BLAST
    Regioni237 – 349113Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5MQD1-1 [UniParc]FASTAAdd to Basket

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    MLIIFLFFYF CYGFNEPLNV VSHLNHDWFL FGDSRSDCNH INNLKIKNFD    50
    YLDIHPSLCN NGKISSSAGD SIFKSFHFTR FYNYTGEGDQ IIFYEGVNFN 100
    PYHRFKCFPN GSNDVWLLNK VRFYRALYSN MAFFRYLTFV DIPYNVSLSK 150
    FNSCKSDILS LNNPIFINYS KEVYFTLLGC SLYLVPLCLF KSNFSQYYYN 200
    IDTGSVYGFS NVVYPDLDCI YISLKPGSYK VSTTAPFLSL PTKALCFDKS 250
    KQFVPVQVVD SRWNNERASD ISLSVACQLP YCYFRNSSAN YVGKYDINHG 300
    DSGFISILSG LLYNVSCISY YGVFLYDNFT SIWPYYSFGR CPTSSIIKHP 350
    ICVYDFLPII LQGILLCLAL LFVVFLLFLL YNDKSH 386
    Length:386
    Mass (Da):44,679
    Last modified:February 1, 2005 - v1
    Checksum:i8A880856BCDE8818
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY597011 Genomic RNA. Translation: AAT98579.1.
    RefSeqiYP_173237.1. NC_006577.2.

    Genome annotation databases

    GeneIDi3200425.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY597011 Genomic RNA. Translation: AAT98579.1 .
    RefSeqi YP_173237.1. NC_006577.2.

    3D structure databases

    ProteinModelPortali Q5MQD1.
    SMRi Q5MQD1. Positions 15-345.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3200425.

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia."
      Woo P.C.Y., Lau S.K.P., Chu C.-M., Chan K.-H., Tsoi H.-W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J.J., Luk W.-K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M., Yuen K.-Y.
      J. Virol. 79:884-895(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiHEMA_CVHN1
    AccessioniPrimary (citable) accession number: Q5MQD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 21, 2007
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Isolate N1 belongs to genotype A.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3