SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5MQD1

- HEMA_CVHN1

UniProt

Q5MQD1 - HEMA_CVHN1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hemagglutinin-esterase

Gene
HE, 2
Organism
Human coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341Nucleophile By similarity
Active sitei202 – 2021Charge relay system By similarity
Active sitei299 – 2991Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2
OrganismiHuman coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Taxonomic identifieri443239 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008170: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini14 – 358345Virion surface Reviewed predictionAdd
BLAST
Transmembranei359 – 37921Helical; Reviewed predictionAdd
BLAST
Topological domaini380 – 3867Intravirion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1313 Reviewed predictionAdd
BLAST
Chaini14 – 386373Hemagglutinin-esterasePRO_0000297761Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 59 By similarity
Glycosylationi83 – 831N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi107 ↔ 154 By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi168 – 1681N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi180 ↔ 246 By similarity
Disulfide bondi188 ↔ 219 By similarity
Glycosylationi193 – 1931N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi277 ↔ 282 By similarity
Glycosylationi286 – 2861N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi314 – 3141N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi317 ↔ 341 By similarity
Glycosylationi328 – 3281N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ5MQD1.
SMRiQ5MQD1. Positions 15-345.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 121121Esterase domain first part By similarityAdd
BLAST
Regioni122 – 236115Receptor binding By similarityAdd
BLAST
Regioni237 – 349113Esterase domain second part By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5MQD1-1 [UniParc]FASTAAdd to Basket

« Hide

MLIIFLFFYF CYGFNEPLNV VSHLNHDWFL FGDSRSDCNH INNLKIKNFD    50
YLDIHPSLCN NGKISSSAGD SIFKSFHFTR FYNYTGEGDQ IIFYEGVNFN 100
PYHRFKCFPN GSNDVWLLNK VRFYRALYSN MAFFRYLTFV DIPYNVSLSK 150
FNSCKSDILS LNNPIFINYS KEVYFTLLGC SLYLVPLCLF KSNFSQYYYN 200
IDTGSVYGFS NVVYPDLDCI YISLKPGSYK VSTTAPFLSL PTKALCFDKS 250
KQFVPVQVVD SRWNNERASD ISLSVACQLP YCYFRNSSAN YVGKYDINHG 300
DSGFISILSG LLYNVSCISY YGVFLYDNFT SIWPYYSFGR CPTSSIIKHP 350
ICVYDFLPII LQGILLCLAL LFVVFLLFLL YNDKSH 386
Length:386
Mass (Da):44,679
Last modified:February 1, 2005 - v1
Checksum:i8A880856BCDE8818
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY597011 Genomic RNA. Translation: AAT98579.1.
RefSeqiYP_173237.1. NC_006577.2.

Genome annotation databases

GeneIDi3200425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY597011 Genomic RNA. Translation: AAT98579.1 .
RefSeqi YP_173237.1. NC_006577.2.

3D structure databases

ProteinModelPortali Q5MQD1.
SMRi Q5MQD1. Positions 15-345.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3200425.

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia."
    Woo P.C.Y., Lau S.K.P., Chu C.-M., Chan K.-H., Tsoi H.-W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J.J., Luk W.-K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M., Yuen K.-Y.
    J. Virol. 79:884-895(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_CVHN1
AccessioniPrimary (citable) accession number: Q5MQD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Isolate N1 belongs to genotype A.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi