Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase DCLK2

Gene

Dclk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase with a significantly reduced Ca2++/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei438 – 4381ATPPROSITE-ProRule annotation
Active sitei530 – 5301Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi415 – 4239ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase DCLK2 (EC:2.7.11.1)
Alternative name(s):
CaMK-like CREB regulatory kinase 2
Short name:
CL2
Short name:
CLICK-II
Short name:
CLICK2
Doublecortin-like and CAM kinase-like 2
Doublecortin-like kinase 2
Gene namesi
Name:Dclk2
Synonyms:Dcamkl2, Dck2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1308384. Dclk2.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication

  • Note: Colocalizes with microtubules. When overexpressed in sympathetic neurons, localizes to cell body and to the terminal segments of axons and dendrites.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi438 – 4381K → A: Loss of autophosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Serine/threonine-protein kinase DCLK2PRO_0000393224Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei61 – 611PhosphothreonineBy similarity
Modified residuei377 – 3771PhosphoserineBy similarity
Modified residuei662 – 6621PhosphoserineCombined sources
Modified residuei681 – 6811PhosphothreonineCombined sources

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5MPA9.

PTM databases

iPTMnetiQ5MPA9.
PhosphoSiteiQ5MPA9.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016550.
ExpressionAtlasiQ5MPA9. baseline and differential.
GenevisibleiQ5MPA9. RN.

Interactioni

Subunit structurei

Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin (By similarity). Binds to and stabilizes microtubules; binding affinity is strongly reduced by autophosphorylation.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053894.

Structurei

3D structure databases

ProteinModelPortaliQ5MPA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 15887Doublecortin 1PROSITE-ProRule annotationAdd
BLAST
Domaini196 – 27984Doublecortin 2PROSITE-ProRule annotationAdd
BLAST
Domaini409 – 666258Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi292 – 37786Ser-richAdd
BLAST
Compositional biasi739 – 75113Pro-richAdd
BLAST

Domaini

The doublecortin domains are involved in the binding to microtubules.

Sequence similaritiesi

Contains 2 doublecortin domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000230855.
HOVERGENiHBG003790.
InParanoidiQ5MPA9.
KOiK08805.
OMAiECIDRST.
OrthoDBiEOG091G02RF.
PhylomeDBiQ5MPA9.
TreeFamiTF318770.

Family and domain databases

CDDicd01617. DCX. 2 hits.
Gene3Di3.10.20.230. 2 hits.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5MPA9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTRSIELE HFEERDKRPR PGSRRGAPSS SGGSSISGPK GNGLIPSPAH
60 70 80 90 100
SAHCSFYRTR TLQALSSEKK AKKARFYRNG DRYFKGLVFA ISSDRFRSFD
110 120 130 140 150
ALLIELTRSL SDNVNLPQGV RTIYTVDGSR KVTSLDELLE GESYVCASNE
160 170 180 190 200
PFRKVDYTKN VNPNWSVNIK GGTTRTLAVA SAKSEVKESK DFIKPKLVTV
210 220 230 240 250
IRSGVKPRKA VRILLNKKTA HSFEQVLTDI TEAIKLDSGV VKRLCTLDGK
260 270 280 290 300
QVTCLQDFFG DDDVFIACGP EKYRYAQDDF VLDHSECRVL KSSYSRASAA
310 320 330 340 350
KYSGSRSPGL SRRSKSPASV KRAGHSSAYS TAKSPVNGTP SSQLSTPKST
360 370 380 390 400
KSSSSSPTSP GSFRGLKQIS AQGRSSSNVN GGPELDRCMS PEGVNGNRCS
410 420 430 440 450
ESFTLLEKYR IGKVIGDGNF AVVKECMDRS TGKEFALKII DKAKCCGKEH
460 470 480 490 500
LIENEVSILR RVKHPNIIML VEEMETTTEL FLVMELVKGG DLFDAITSST
510 520 530 540 550
KYTERDGSAM VYNLASALRY LHGLSIVHRD IKPENLLVCE YPDGTKSLKL
560 570 580 590 600
GDFGLATVVE GPLYTVCGTP TYVAPEIIAE TGYGLKVDVW AAGVITYILL
610 620 630 640 650
CGFPPFRSEN NLQEDLFDQI LAGKLEFPAP YWDNITDSAK ELISQMLQVN
660 670 680 690 700
VEARCTAGEI LSHPWVSDDA SQENNMQAEV TGKLKQHFNN ALPKQNSTTT
710 720 730 740 750
GVSVIMNTAL DKEGQVFCSK HCRDSSKSSR EQTSAREAPP PPESPRPPGP
760
PATSGCDPAG TWRRHRD
Length:767
Mass (Da):84,016
Last modified:March 23, 2010 - v2
Checksum:i2348331B01FF5B79
GO
Isoform 2 (identifier: Q5MPA9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     707-715: NTALDKEGQ → VQGHEHGSR
     716-767: Missing.

Note: No experimental confirmation available.
Show »
Length:715
Mass (Da):78,394
Checksum:iB33DF51C3E0C8D2A
GO
Isoform 3 (identifier: Q5MPA9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-335: Missing.

Show »
Length:751
Mass (Da):82,387
Checksum:iC5A792EFC6382ABD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261V → I in AAV85464 (PubMed:15611072).Curated
Sequence conflicti308 – 3081P → L in AAV85464 (PubMed:15611072).Curated
Sequence conflicti387 – 3871R → C in AAV85461 (PubMed:15611072).Curated
Sequence conflicti387 – 3871R → C in AAV85462 (PubMed:15611072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei320 – 33516Missing in isoform 3. 1 PublicationVSP_038897Add
BLAST
Alternative sequencei707 – 7159NTALDKEGQ → VQGHEHGSR in isoform 2. 1 PublicationVSP_038898
Alternative sequencei716 – 76752Missing in isoform 2. 1 PublicationVSP_038899Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY673997 mRNA. Translation: AAV85461.1.
AY673998 mRNA. Translation: AAV85462.1.
AY673999 mRNA. Translation: AAV85463.1.
AY674000 mRNA. Translation: AAV85464.1.
RefSeqiNP_001009691.3. NM_001009691.3. [Q5MPA9-1]
NP_001182761.1. NM_001195832.1. [Q5MPA9-2]
XP_006232741.1. XM_006232679.2. [Q5MPA9-1]
XP_006232743.1. XM_006232681.2. [Q5MPA9-3]
UniGeneiRn.23327.

Genome annotation databases

EnsembliENSRNOT00000057062; ENSRNOP00000053894; ENSRNOG00000016550. [Q5MPA9-1]
ENSRNOT00000066004; ENSRNOP00000062805; ENSRNOG00000016550. [Q5MPA9-1]
ENSRNOT00000079954; ENSRNOP00000075203; ENSRNOG00000016550. [Q5MPA9-2]
ENSRNOT00000089339; ENSRNOP00000074906; ENSRNOG00000016550. [Q5MPA9-2]
GeneIDi310698.
KEGGirno:310698.
UCSCiRGD:1308384. rat. [Q5MPA9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY673997 mRNA. Translation: AAV85461.1.
AY673998 mRNA. Translation: AAV85462.1.
AY673999 mRNA. Translation: AAV85463.1.
AY674000 mRNA. Translation: AAV85464.1.
RefSeqiNP_001009691.3. NM_001009691.3. [Q5MPA9-1]
NP_001182761.1. NM_001195832.1. [Q5MPA9-2]
XP_006232741.1. XM_006232679.2. [Q5MPA9-1]
XP_006232743.1. XM_006232681.2. [Q5MPA9-3]
UniGeneiRn.23327.

3D structure databases

ProteinModelPortaliQ5MPA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053894.

PTM databases

iPTMnetiQ5MPA9.
PhosphoSiteiQ5MPA9.

Proteomic databases

PaxDbiQ5MPA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000057062; ENSRNOP00000053894; ENSRNOG00000016550. [Q5MPA9-1]
ENSRNOT00000066004; ENSRNOP00000062805; ENSRNOG00000016550. [Q5MPA9-1]
ENSRNOT00000079954; ENSRNOP00000075203; ENSRNOG00000016550. [Q5MPA9-2]
ENSRNOT00000089339; ENSRNOP00000074906; ENSRNOG00000016550. [Q5MPA9-2]
GeneIDi310698.
KEGGirno:310698.
UCSCiRGD:1308384. rat. [Q5MPA9-1]

Organism-specific databases

CTDi166614.
RGDi1308384. Dclk2.

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000230855.
HOVERGENiHBG003790.
InParanoidiQ5MPA9.
KOiK08805.
OMAiECIDRST.
OrthoDBiEOG091G02RF.
PhylomeDBiQ5MPA9.
TreeFamiTF318770.

Miscellaneous databases

PROiQ5MPA9.

Gene expression databases

BgeeiENSRNOG00000016550.
ExpressionAtlasiQ5MPA9. baseline and differential.
GenevisibleiQ5MPA9. RN.

Family and domain databases

CDDicd01617. DCX. 2 hits.
Gene3Di3.10.20.230. 2 hits.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCLK2_RAT
AccessioniPrimary (citable) accession number: Q5MPA9
Secondary accession number(s): Q5MPA7, Q5MPA8, Q5MPB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: March 23, 2010
Last modified: September 7, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.