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Q5MJS3 (DMP4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Extracellular serine/threonine protein kinase FAM20C

EC=2.7.11.1
Alternative name(s):
Dentin matrix protein 4
Short name=DMP-4
Golgi-enriched fraction casein kinase
Short name=GEF-CK
Gene names
Name:Fam20c
Synonyms:Dmp4, Kiaa4081
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length579 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-binding kinase that phosphorylates the caseins and several secreted proteins implicated in biomineralization, including the secretory calcium binding phosphoproteins (SCPP). Preferencially phosphorylates its targets within the S-x-E/pS motif By similarity. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Secreted Ref.1.

Tissue specificity

Highly expressed in the tooth. No expression in the dental pulp. At the secretory stage of amelogenesis, it is detected in the matrix of the enamel, in the ameloblasts, and within the cells adjoining the stratum intermedium (a tissue layer analogous to the stellate reticulum seen in the developing molar). Strong expression is observed in maturation stage ameloblasts and throughout the non-cornified layers of the gingival epithelium. Expressed at moderate levels in bone and at low levels in kidney, liver, brain and lung. Very low expression, if any, in spleen and skeletal muscle. Ref.1 Ref.5

Developmental stage

In the developing tooth, initial expression observed in odontoblasts at all stages of development. At later stages, restricted expression pattern in ameloblasts. Also observed in osteoblats in the alveolar bone. Ref.1

Sequence similarities

Belongs to the FAM20 family.

Sequence caution

The sequence AAH04044.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAH04044.3 differs from that shown. Reason: Frameshift at position 294.

The sequence AAH25814.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH25826.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAD90211.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAD90211.1 differs from that shown. Reason: Frameshift at position 296.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 579553Extracellular serine/threonine protein kinase FAM20C
PRO_0000297978

Regions

Region349 – 560212Kinase domain By similarity

Sites

Metal binding4581Manganese By similarity
Metal binding4731Manganese By similarity

Amino acid modifications

Glycosylation961N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3331K → N in CAJ18570. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q5MJS3 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 9DE5F5496AC117FE

FASTA57965,766
        10         20         30         40         50         60 
MKMILVRRFR VLILVVFLLA CALHIAVDLL PKLDRRATRS SGEPGCSCAQ PAAEAAGPGW 

        70         80         90        100        110        120 
AQARSRPGES AGGDAGWPNK HTLRILQDFS SDPASNLTSH SLEKLPSAAE PVDHAPRGQE 

       130        140        150        160        170        180 
PRSPPPRDPA HRPLLRDPGP RPRVPPPGPS GDGSLLAKLF EHPLYQGAVP PLTEDDVLFN 

       190        200        210        220        230        240 
VNSDIRFNPK AAENPDWPHE GAEGAEFLPT GEAAVNLYPN WLKFHIGINR YELYSRHNPA 

       250        260        270        280        290        300 
IDALLRDLGS QKITSVAMKS GGTQLKLIMT FQNYGQALFK PMKQTREQET PPDFFYFSDY 

       310        320        330        340        350        360 
ERHNAEIAAF HLDRILDFRR VPPVAGRMIN MTKEIRDVTR DKKLWRTFFV SPANNICFYG 

       370        380        390        400        410        420 
ECSYYCSTEH ALCGRPDQIE GSLAAFLPDL SLAKRKTWRN PWRRSYHKRK KAEWEVDPDY 

       430        440        450        460        470        480 
CEEVKQTPPY DSGHRILDIM DMTVFDFLMG NMDRHHYETF EKFGNETFII HLDNGRGFGK 

       490        500        510        520        530        540 
YSHDELSILA PLHQCCRIRR STYLRLQLLA KEEHKLSLLM AESLQHDKVA PVLYQLHLEA 

       550        560        570 
LDRRLRIVLQ AVRDCVEKDG LSSVVEDDLA TEHRASTER 

« Hide

References

« Hide 'large scale' references
[1]"Dentin matrix protein 4, a novel secretory calcium-binding protein that modulates odontoblast differentiation."
Hao J., Narayanan K., Muni T., Ramachandran A., George A.
J. Biol. Chem. 282:15357-15365(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Seino S., Nishimura M., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreatic islet.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver and Mammary tumor.
[4]"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 331-579.
[5]"Whole-exome sequencing identifies FAM20A mutations as a cause of amelogenesis imperfecta and gingival hyperplasia syndrome."
O'Sullivan J., Bitu C.C., Daly S.B., Urquhart J.E., Barron M.J., Bhaskar S.S., Martelli-Junior H., dos Santos Neto P.E., Mansilla M.A., Murray J.C., Coletta R.D., Black G.C., Dixon M.J.
Am. J. Hum. Genet. 88:616-620(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY778962 mRNA. Translation: AAV91326.1.
AK220286 mRNA. Translation: BAD90211.1. Sequence problems.
BC004044 mRNA. Translation: AAH04044.3. Sequence problems.
BC025814 mRNA. Translation: AAH25814.1. Different initiation.
BC025826 mRNA. Translation: AAH25826.1. Different initiation.
CT010363 mRNA. Translation: CAJ18570.1.
CCDSCCDS51681.1.
RefSeqNP_085042.2. NM_030565.6.
UniGeneMm.320355.

3D structure databases

ProteinModelPortalQ5MJS3.
SMRQ5MJS3. Positions 220-565.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000026972.

PTM databases

PhosphoSiteQ5MJS3.

Proteomic databases

PaxDbQ5MJS3.
PRIDEQ5MJS3.

Protocols and materials databases

DNASU80752.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026972; ENSMUSP00000026972; ENSMUSG00000025854.
GeneID80752.
KEGGmmu:80752.
UCSCuc009afv.2. mouse.

Organism-specific databases

CTD56975.
MGIMGI:2136853. Fam20c.
RougeSearch...

Phylogenomic databases

eggNOGNOG238678.
GeneTreeENSGT00390000007484.
HOGENOMHOG000231437.
HOVERGENHBG051635.
InParanoidQ5MJS3.
OMAPDWPREG.
OrthoDBEOG7JX33T.
PhylomeDBQ5MJS3.
TreeFamTF313276.

Gene expression databases

ArrayExpressQ5MJS3.
BgeeQ5MJS3.
GenevestigatorQ5MJS3.

Family and domain databases

InterProIPR009581. DUF1193.
IPR024869. FAM20.
[Graphical view]
PANTHERPTHR12450. PTHR12450. 1 hit.
PfamPF06702. DUF1193. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFAM20C. mouse.
NextBio350121.
PROQ5MJS3.
SOURCESearch...

Entry information

Entry nameDMP4_MOUSE
AccessionPrimary (citable) accession number: Q5MJS3
Secondary accession number(s): Q4FJP0 expand/collapse secondary AC list , Q571A3, Q6PKA8, Q8JZP7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: February 1, 2005
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot