Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5MJS3

- DMP4_MOUSE

UniProt

Q5MJS3 - DMP4_MOUSE

Protein

Extracellular serine/threonine protein kinase FAM20C

Gene

Fam20c

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Calcium-binding kinase that phosphorylates the caseins and several secreted proteins implicated in biomineralization, including the secretory calcium binding phosphoproteins (SCPP). Preferencially phosphorylates its targets within the S-x-E/pS motif By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi458 – 4581ManganeseBy similarity
    Metal bindingi473 – 4731ManganeseBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: MGI
    2. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. dentinogenesis Source: MGI
    2. enamel mineralization Source: MGI
    3. odontoblast differentiation Source: MGI
    4. osteoclast maturation Source: MGI
    5. positive regulation of bone mineralization Source: MGI
    6. positive regulation of osteoblast differentiation Source: MGI
    7. regulation of fibroblast growth factor receptor signaling pathway Source: MGI
    8. regulation of phosphorus metabolic process Source: MGI
    9. skeletal system development Source: MGI

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Biomineralization

    Keywords - Ligandi

    Calcium, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Extracellular serine/threonine protein kinase FAM20C (EC:2.7.11.1)
    Alternative name(s):
    Dentin matrix protein 4
    Short name:
    DMP-4
    Golgi-enriched fraction casein kinase
    Short name:
    GEF-CK
    Gene namesi
    Name:Fam20c
    Synonyms:Dmp4, Kiaa4081
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:2136853. Fam20c.

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 579553Extracellular serine/threonine protein kinase FAM20CPRO_0000297978Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ5MJS3.
    PRIDEiQ5MJS3.

    PTM databases

    PhosphoSiteiQ5MJS3.

    Expressioni

    Tissue specificityi

    Highly expressed in the tooth. No expression in the dental pulp. At the secretory stage of amelogenesis, it is detected in the matrix of the enamel, in the ameloblasts, and within the cells adjoining the stratum intermedium (a tissue layer analogous to the stellate reticulum seen in the developing molar). Strong expression is observed in maturation stage ameloblasts and throughout the non-cornified layers of the gingival epithelium. Expressed at moderate levels in bone and at low levels in kidney, liver, brain and lung. Very low expression, if any, in spleen and skeletal muscle.2 Publications

    Developmental stagei

    In the developing tooth, initial expression observed in odontoblasts at all stages of development. At later stages, restricted expression pattern in ameloblasts. Also observed in osteoblats in the alveolar bone.1 Publication

    Gene expression databases

    ArrayExpressiQ5MJS3.
    BgeeiQ5MJS3.
    GenevestigatoriQ5MJS3.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000026972.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5MJS3.
    SMRiQ5MJS3. Positions 220-565.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni349 – 560212Kinase domainBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the FAM20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG238678.
    GeneTreeiENSGT00390000007484.
    HOGENOMiHOG000231437.
    HOVERGENiHBG051635.
    InParanoidiQ5MJS3.
    OMAiPDWPREG.
    OrthoDBiEOG7JX33T.
    PhylomeDBiQ5MJS3.
    TreeFamiTF313276.

    Family and domain databases

    InterProiIPR009581. DUF1193.
    IPR024869. FAM20.
    [Graphical view]
    PANTHERiPTHR12450. PTHR12450. 1 hit.
    PfamiPF06702. DUF1193. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5MJS3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMILVRRFR VLILVVFLLA CALHIAVDLL PKLDRRATRS SGEPGCSCAQ    50
    PAAEAAGPGW AQARSRPGES AGGDAGWPNK HTLRILQDFS SDPASNLTSH 100
    SLEKLPSAAE PVDHAPRGQE PRSPPPRDPA HRPLLRDPGP RPRVPPPGPS 150
    GDGSLLAKLF EHPLYQGAVP PLTEDDVLFN VNSDIRFNPK AAENPDWPHE 200
    GAEGAEFLPT GEAAVNLYPN WLKFHIGINR YELYSRHNPA IDALLRDLGS 250
    QKITSVAMKS GGTQLKLIMT FQNYGQALFK PMKQTREQET PPDFFYFSDY 300
    ERHNAEIAAF HLDRILDFRR VPPVAGRMIN MTKEIRDVTR DKKLWRTFFV 350
    SPANNICFYG ECSYYCSTEH ALCGRPDQIE GSLAAFLPDL SLAKRKTWRN 400
    PWRRSYHKRK KAEWEVDPDY CEEVKQTPPY DSGHRILDIM DMTVFDFLMG 450
    NMDRHHYETF EKFGNETFII HLDNGRGFGK YSHDELSILA PLHQCCRIRR 500
    STYLRLQLLA KEEHKLSLLM AESLQHDKVA PVLYQLHLEA LDRRLRIVLQ 550
    AVRDCVEKDG LSSVVEDDLA TEHRASTER 579
    Length:579
    Mass (Da):65,766
    Last modified:February 1, 2005 - v1
    Checksum:i9DE5F5496AC117FE
    GO

    Sequence cautioni

    The sequence AAH04044.3 differs from that shown. Reason: Frameshift at position 294.
    The sequence BAD90211.1 differs from that shown. Reason: Frameshift at position 296.
    The sequence AAH04044.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAH25814.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH25826.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAD90211.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti333 – 3331K → N in CAJ18570. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY778962 mRNA. Translation: AAV91326.1.
    AK220286 mRNA. Translation: BAD90211.1. Sequence problems.
    BC004044 mRNA. Translation: AAH04044.3. Sequence problems.
    BC025814 mRNA. Translation: AAH25814.1. Different initiation.
    BC025826 mRNA. Translation: AAH25826.1. Different initiation.
    CT010363 mRNA. Translation: CAJ18570.1.
    CCDSiCCDS51681.1.
    RefSeqiNP_085042.2. NM_030565.6.
    UniGeneiMm.320355.

    Genome annotation databases

    EnsembliENSMUST00000026972; ENSMUSP00000026972; ENSMUSG00000025854.
    GeneIDi80752.
    KEGGimmu:80752.
    UCSCiuc009afv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY778962 mRNA. Translation: AAV91326.1 .
    AK220286 mRNA. Translation: BAD90211.1 . Sequence problems.
    BC004044 mRNA. Translation: AAH04044.3 . Sequence problems.
    BC025814 mRNA. Translation: AAH25814.1 . Different initiation.
    BC025826 mRNA. Translation: AAH25826.1 . Different initiation.
    CT010363 mRNA. Translation: CAJ18570.1 .
    CCDSi CCDS51681.1.
    RefSeqi NP_085042.2. NM_030565.6.
    UniGenei Mm.320355.

    3D structure databases

    ProteinModelPortali Q5MJS3.
    SMRi Q5MJS3. Positions 220-565.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000026972.

    PTM databases

    PhosphoSitei Q5MJS3.

    Proteomic databases

    PaxDbi Q5MJS3.
    PRIDEi Q5MJS3.

    Protocols and materials databases

    DNASUi 80752.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026972 ; ENSMUSP00000026972 ; ENSMUSG00000025854 .
    GeneIDi 80752.
    KEGGi mmu:80752.
    UCSCi uc009afv.2. mouse.

    Organism-specific databases

    CTDi 56975.
    MGIi MGI:2136853. Fam20c.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG238678.
    GeneTreei ENSGT00390000007484.
    HOGENOMi HOG000231437.
    HOVERGENi HBG051635.
    InParanoidi Q5MJS3.
    OMAi PDWPREG.
    OrthoDBi EOG7JX33T.
    PhylomeDBi Q5MJS3.
    TreeFami TF313276.

    Miscellaneous databases

    ChiTaRSi FAM20C. mouse.
    NextBioi 350121.
    PROi Q5MJS3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q5MJS3.
    Bgeei Q5MJS3.
    Genevestigatori Q5MJS3.

    Family and domain databases

    InterProi IPR009581. DUF1193.
    IPR024869. FAM20.
    [Graphical view ]
    PANTHERi PTHR12450. PTHR12450. 1 hit.
    Pfami PF06702. DUF1193. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dentin matrix protein 4, a novel secretory calcium-binding protein that modulates odontoblast differentiation."
      Hao J., Narayanan K., Muni T., Ramachandran A., George A.
      J. Biol. Chem. 282:15357-15365(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Seino S., Nishimura M., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreatic islet.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver and Mammary tumor.
    4. "Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
      Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 331-579.
    5. "Whole-exome sequencing identifies FAM20A mutations as a cause of amelogenesis imperfecta and gingival hyperplasia syndrome."
      O'Sullivan J., Bitu C.C., Daly S.B., Urquhart J.E., Barron M.J., Bhaskar S.S., Martelli-Junior H., dos Santos Neto P.E., Mansilla M.A., Murray J.C., Coletta R.D., Black G.C., Dixon M.J.
      Am. J. Hum. Genet. 88:616-620(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiDMP4_MOUSE
    AccessioniPrimary (citable) accession number: Q5MJS3
    Secondary accession number(s): Q4FJP0
    , Q571A3, Q6PKA8, Q8JZP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 21, 2007
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3