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Q5MJ70 (SPDYA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Speedy protein A
Alternative name(s):
Rapid inducer of G2/M progression in oocytes A
Short name=RINGO A
Short name=hSpy/Ringo A
Speedy-1
Short name=Spy1
Gene names
Name:SPDYA
Synonyms:SPDY1, SPY1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2. Ref.1 Ref.5 Ref.6 UniProtKB Q5IBH7

Subunit structure

Interacts with CDK1, CDK2 and CDKN1B/KIP1. Found in a complex with both CDK2 and CDKN1B/KIP1. Ref.1 Ref.5 Ref.6

Subcellular location

Nucleus Ref.1 Ref.6.

Tissue specificity

Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus. Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase. Ref.1 Ref.2

Domain

The C-terminus is required for CDK2-activation, but not CDK2-binding By similarity. UniProtKB Q5IBH7

Sequence similarities

Belongs to the Speedy/Ringo family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 Ref.2 Ref.3 (identifier: Q5MJ70-2)

Also known as: A2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 Ref.2 (identifier: Q5MJ70-1)

Also known as: A1;

The sequence of this isoform differs from the canonical sequence as follows:
     284-286: VVN → GMI
     287-313: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Speedy protein A
PRO_0000234112

Regions

Region68 – 200133Speedy/Ringo box; Required for CDK-binding By similarity UniProtKB Q5IBH7

Natural variations

Alternative sequence284 – 2863VVN → GMI in isoform 1. Ref.2
VSP_052027
Alternative sequence287 – 31327Missing in isoform 1. Ref.2
VSP_052028

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (A2) [UniParc].

Last modified May 2, 2006. Version 2.
Checksum: F511EECB26245EEC

FASTA31336,463
        10         20         30         40         50         60 
MRHNQMCCET PPTVTVYVKS GSNRSHQPKK PITLKRPICK DNWQAFEKNT HNNNKSKRPK 

        70         80         90        100        110        120 
GPCLVIQRQD MTAFFKLFDD DLIQDFLWMD CCCKIADKYL LAMTFVYFKR AKFTISEHTR 

       130        140        150        160        170        180 
INFFIALYLA NTVEEDEEET KYEIFPWALG KNWRKLFPNF LKLRDQLWDR IDYRAIVSRR 

       190        200        210        220        230        240 
CCEEVMAIAP THYIWQRERS VHHSGAVRNY NRDEVQLPRG PSATPVDCSL CGKKRRYVRL 

       250        260        270        280        290        300 
GLSSSSSLSS HTAGVTEKHS QDSYNSLSMD IIGDPSQAYT GSEVVNDHQS NKGKKTNFLK 

       310 
KDKSMEWFTG SEE 

« Hide

Isoform 1 (A1) [UniParc].

Checksum: 348BAE62F09024E9
Show »

FASTA28633,269

References

« Hide 'large scale' references
[1]"Human Speedy: a novel cell cycle regulator that enhances proliferation through activation of Cdk2."
Porter L.A., Dellinger R.W., Tynan J.A., Barnes E.A., Kong M., Lenormand J.-L., Donoghue D.J.
J. Cell Biol. 157:357-366(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH CDK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Testis.
[2]"Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins."
Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.
Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
Tissue: Testis.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[5]"Human Spy1 promotes survival of mammalian cells following DNA damage."
Barnes E.A., Porter L.A., Lenormand J.-L., Dellinger R.W., Donoghue D.J.
Cancer Res. 63:3701-3707(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDK2.
[6]"Spy1 interacts with p27Kip1 to allow G1/S progression."
Porter L.A., Kong-Beltran M., Donoghue D.J.
Mol. Biol. Cell 14:3664-3674(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDKN1B, IDENTIFICATION IN A COMPLEX WITH CDKN1B AND CDK2, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY820303 mRNA. Translation: AAW30394.1.
AY820304 mRNA. Translation: AAW30395.1.
AC097720 Genomic DNA. Translation: AAY24014.1.
BC093005 mRNA. Translation: AAH93005.1.
RefSeqNP_001008779.1. NM_001008779.1.
NP_001136106.1. NM_001142634.1.
NP_877433.2. NM_182756.3.
UniGeneHs.511956.

3D structure databases

ProteinModelPortalQ5MJ70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128828. 5 interactions.
IntActQ5MJ70. 2 interactions.
MINTMINT-7226374.
STRING9606.ENSP00000335628.

PTM databases

PhosphoSiteQ5MJ70.

Polymorphism databases

DMDM94730574.

Proteomic databases

PaxDbQ5MJ70.
PRIDEQ5MJ70.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000334056; ENSP00000335628; ENSG00000163806. [Q5MJ70-2]
ENST00000379579; ENSP00000368898; ENSG00000163806. [Q5MJ70-2]
GeneID245711.
KEGGhsa:245711.
UCSCuc002rmi.3. human. [Q5MJ70-1]
uc002rmj.3. human. [Q5MJ70-2]

Organism-specific databases

CTD245711.
GeneCardsGC02P029007.
H-InvDBHIX0021816.
HGNCHGNC:30613. SPDYA.
MIM614029. gene.
neXtProtNX_Q5MJ70.
PharmGKBPA134954857.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42793.
HOGENOMHOG000060281.
HOVERGENHBG086798.
InParanoidQ5MJ70.
KOK08694.
OMAKHSQESH.
OrthoDBEOG7PS1FZ.
PhylomeDBQ5MJ70.
TreeFamTF329827.

Gene expression databases

ArrayExpressQ5MJ70.
BgeeQ5MJ70.
CleanExHS_SPDYA.
GenevestigatorQ5MJ70.

Family and domain databases

InterProIPR020984. Cell_cycle_regulatory_Spy1.
[Graphical view]
PfamPF11357. Spy1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSPDYA. human.
GeneWikiSPDYA.
GenomeRNAi245711.
NextBio91722.
PROQ5MJ70.
SOURCESearch...

Entry information

Entry nameSPDYA_HUMAN
AccessionPrimary (citable) accession number: Q5MJ70
Secondary accession number(s): Q53R05, Q5MJ69
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: April 16, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM