Q5MJ70 (SPDYA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Speedy protein A

Alternative name(s):
Rapid inducer of G2/M progression in oocytes A
Short name=RINGO A
Short name=hSpy/Ringo A
Speedy-1
Short name=Spy1

Gene names

Name:	SPDYA
Synonyms:	SPDY1, SPY1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2. Ref.1 Ref.5 Ref.6 UniProtKB Q5IBH7
Subunit structure	Interacts with CDK1, CDK2 and CDKN1B/KIP1. Found in a complex with both CDK2 and CDKN1B/KIP1. Ref.1 Ref.5 Ref.6
Subcellular location	Nucleus Ref.1 Ref.6.
Tissue specificity	Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus. Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase. Ref.1 Ref.2
Domain	The C-terminus is required for CDK2-activation, but not CDK2-binding By similarity. UniProtKB Q5IBH7
Sequence similarities	Belongs to the Speedy/Ringo family.

Ontologies

Keywords
Biological process	Cell cycle DNA damage
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from direct assay Ref.1. Source: UniProtKB male meiosis Inferred from sequence or structural similarity. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell proliferation Inferred from direct assay Ref.1. Source: UniProtKB positive regulation of cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: Compara response to DNA damage stimulus Inferred from direct assay Ref.5. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 Ref.2 Ref.3 (identifier: Q5MJ70-2)

Also known as: A2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 Ref.2 (identifier: Q5MJ70-1)

Also known as: A1;

The sequence of this isoform differs from the canonical sequence as follows:
     284-286: VVN → GMI
     287-313: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Speedy protein A		PRO_0000234112
Regions
	Region	68 – 200	133	Speedy/Ringo box; Required for CDK-binding By similarity UniProtKB Q5IBH7
Natural variations
	Alternative sequence	284 – 286	3	VVN → GMI in isoform 1. Ref.2		VSP_052027
	Alternative sequence	287 – 313	27	Missing in isoform 1. Ref.2		VSP_052028

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 (A2) [UniParc]. Last modified May 2, 2006. Version 2. Checksum: F511EECB26245EEC	FASTA	313	36,463
	10 20 30 40 50 60 MRHNQMCCET PPTVTVYVKS GSNRSHQPKK PITLKRPICK DNWQAFEKNT HNNNKSKRPK 70 80 90 100 110 120 GPCLVIQRQD MTAFFKLFDD DLIQDFLWMD CCCKIADKYL LAMTFVYFKR AKFTISEHTR 130 140 150 160 170 180 INFFIALYLA NTVEEDEEET KYEIFPWALG KNWRKLFPNF LKLRDQLWDR IDYRAIVSRR 190 200 210 220 230 240 CCEEVMAIAP THYIWQRERS VHHSGAVRNY NRDEVQLPRG PSATPVDCSL CGKKRRYVRL 250 260 270 280 290 300 GLSSSSSLSS HTAGVTEKHS QDSYNSLSMD IIGDPSQAYT GSEVVNDHQS NKGKKTNFLK 310 KDKSMEWFTG SEE
	Isoform 1 (A1) [UniParc]. Checksum: 348BAE62F09024E9	FASTA	286	33,269
	10 20 30 40 50 60 MRHNQMCCET PPTVTVYVKS GSNRSHQPKK PITLKRPICK DNWQAFEKNT HNNNKSKRPK 70 80 90 100 110 120 GPCLVIQRQD MTAFFKLFDD DLIQDFLWMD CCCKIADKYL LAMTFVYFKR AKFTISEHTR 130 140 150 160 170 180 INFFIALYLA NTVEEDEEET KYEIFPWALG KNWRKLFPNF LKLRDQLWDR IDYRAIVSRR 190 200 210 220 230 240 CCEEVMAIAP THYIWQRERS VHHSGAVRNY NRDEVQLPRG PSATPVDCSL CGKKRRYVRL 250 260 270 280 GLSSSSSLSS HTAGVTEKHS QDSYNSLSMD IIGDPSQAYT GSEGMI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human Speedy: a novel cell cycle regulator that enhances proliferation through activation of Cdk2." Porter L.A., Dellinger R.W., Tynan J.A., Barnes E.A., Kong M., Lenormand J.-L., Donoghue D.J. J. Cell Biol. 157:357-366(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH CDK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins." Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J. Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Testis.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[5]	"Human Spy1 promotes survival of mammalian cells following DNA damage." Barnes E.A., Porter L.A., Lenormand J.-L., Dellinger R.W., Donoghue D.J. Cancer Res. 63:3701-3707(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDK2.
[6]	"Spy1 interacts with p27Kip1 to allow G1/S progression." Porter L.A., Kong-Beltran M., Donoghue D.J. Mol. Biol. Cell 14:3664-3674(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDKN1B, IDENTIFICATION IN A COMPLEX WITH CDKN1B AND CDK2, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY820303 mRNA. Translation: AAW30394.1. AY820304 mRNA. Translation: AAW30395.1. AC097720 Genomic DNA. Translation: AAY24014.1. BC093005 mRNA. Translation: AAH93005.1.
IPI	IPI00549840. IPI00554586.
RefSeq	NP_001008779.1. NM_001008779.1. NP_001136106.1. NM_001142634.1. NP_877433.2. NM_182756.3.
UniGene	Hs.511956.
3D structure databases
ProteinModelPortal	Q5MJ70.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-7226374.
STRING	9606.ENSP00000335628.
PTM databases
PhosphoSite	Q5MJ70.
Polymorphism databases
DMDM	94730574.
Proteomic databases
PaxDb	Q5MJ70.
PRIDE	Q5MJ70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334056; ENSP00000335628; ENSG00000163806. ENST00000379579; ENSP00000368898; ENSG00000163806.
GeneID	245711.
KEGG	hsa:245711.
UCSC	uc002rmi.3. human. uc002rmj.3. human.
Organism-specific databases
CTD	245711.
GeneCards	GC02P029007.
H-InvDB	HIX0021816.
HGNC	HGNC:30613. SPDYA.
MIM	614029. gene.
neXtProt	NX_Q5MJ70.
PharmGKB	PA134954857.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42793.
HOGENOM	HOG000060281.
HOVERGEN	HBG086798.
InParanoid	Q5MJ70.
KO	K08694.
OMA	HSQESHN.
OrthoDB	EOG4KD6MG.
Gene expression databases
ArrayExpress	Q5MJ70.
Bgee	Q5MJ70.
CleanEx	HS_SPDYA.
Genevestigator	Q5MJ70.
GermOnline	ENSG00000163806. Homo sapiens.
Family and domain databases
InterPro	IPR020984. Cell_cycle_regulatory_Spy1. [Graphical view]
Pfam	PF11357. Spy1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SPDYA. human.
GenomeRNAi	245711.
NextBio	91722.
SOURCE	Search...

Entry information

Entry name

SPDYA_HUMAN

Accession

Primary (citable) accession number: Q5MJ70
Secondary accession number(s): Q53R05, Q5MJ69

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	April 3, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.