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Protein

Speedy protein A

Gene

SPDYA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:12972555). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation (PubMed:28666995). Mediates cell survival during the DNA damage process through activation of CDK2 (PubMed:12839962).4 Publications

GO - Molecular functioni

  • protein kinase activator activity Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processCell cycle, DNA damage

Names & Taxonomyi

Protein namesi
Recommended name:
Speedy protein A
Alternative name(s):
Rapid inducer of G2/M progression in oocytes A
Short name:
RINGO A
Short name:
hSpy/Ringo A
Speedy-1
Short name:
Spy1
Gene namesi
Name:SPDYAImported
Synonyms:SPDY1Imported, SPY1Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163806.15.
HGNCiHGNC:30613. SPDYA.
MIMi614029. gene.
neXtProtiNX_Q5MJ70.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97D → N: Loss of CDK2 activation; when associated with Q-135. 1 Publication1
Mutagenesisi135E → Q: Loss of CDK2 activation; when associated with N-97. 1 Publication1

Organism-specific databases

DisGeNETi245711.
OpenTargetsiENSG00000163806.
PharmGKBiPA134954857.

Polymorphism and mutation databases

BioMutaiSPDYA.
DMDMi94730574.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002341121 – 313Speedy protein AAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphoserineBy similarity1
Modified residuei224PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5MJ70.
PeptideAtlasiQ5MJ70.
PRIDEiQ5MJ70.

PTM databases

iPTMnetiQ5MJ70.
PhosphoSitePlusiQ5MJ70.

Expressioni

Tissue specificityi

Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus. Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase.2 Publications

Gene expression databases

BgeeiENSG00000163806.
CleanExiHS_SPDYA.
ExpressionAtlasiQ5MJ70. baseline and differential.

Organism-specific databases

HPAiHPA035162.
HPA066214.

Interactioni

Subunit structurei

Interacts with CDK1 (By similarity). Interacts with CDK2 (PubMed:11980914, PubMed:12839962, PubMed:12972555, PubMed:28666995). May interact with CDKN1B/KIP1 (PubMed:12972555). Identified in a complex with CDK2 and CDKN1B/KIP1, where it interacts primarily with CDK2 (PubMed:12972555, PubMed:28666995).By similarity4 Publications

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi128828. 5 interactors.
IntActiQ5MJ70. 3 interactors.
MINTiMINT-7226374.
STRINGi9606.ENSP00000335628.

Structurei

Secondary structure

1313
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi72 – 75Combined sources4
Helixi76 – 79Combined sources4
Helixi81 – 89Combined sources9
Beta strandi94 – 96Combined sources3
Helixi98 – 110Combined sources13
Helixi115 – 117Combined sources3
Helixi120 – 134Combined sources15
Helixi140 – 143Combined sources4
Helixi144 – 149Combined sources6
Helixi153 – 155Combined sources3
Helixi157 – 170Combined sources14
Turni171 – 173Combined sources3
Helixi179 – 185Combined sources7
Beta strandi187 – 189Combined sources3
Helixi193 – 196Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5UQ1X-ray3.20B/D61-213[»]
5UQ2X-ray2.70B61-213[»]
5UQ3X-ray3.60B61-213[»]
ProteinModelPortaliQ5MJ70.
SMRiQ5MJ70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 200Speedy/Ringo box; Required for CDK-binding1 PublicationAdd BLAST133

Domaini

The C-terminus is required for CDK2-activation, but not CDK2-binding.By similarity

Sequence similaritiesi

Belongs to the Speedy/Ringo family.Curated

Phylogenomic databases

eggNOGiENOG410IWT5. Eukaryota.
ENOG41114KA. LUCA.
GeneTreeiENSGT00420000029723.
HOGENOMiHOG000060281.
HOVERGENiHBG086798.
InParanoidiQ5MJ70.
KOiK08694.
OMAiTNFMKKD.
OrthoDBiEOG091G0CF6.
PhylomeDBiQ5MJ70.
TreeFamiTF329827.

Family and domain databases

InterProiView protein in InterPro
IPR020984. Speedy.
PfamiView protein in Pfam
PF11357. Spy1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 22 Publications (identifier: Q5MJ70-2) [UniParc]FASTAAdd to basket
Also known as: A21 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRHNQMCCET PPTVTVYVKS GSNRSHQPKK PITLKRPICK DNWQAFEKNT
60 70 80 90 100
HNNNKSKRPK GPCLVIQRQD MTAFFKLFDD DLIQDFLWMD CCCKIADKYL
110 120 130 140 150
LAMTFVYFKR AKFTISEHTR INFFIALYLA NTVEEDEEET KYEIFPWALG
160 170 180 190 200
KNWRKLFPNF LKLRDQLWDR IDYRAIVSRR CCEEVMAIAP THYIWQRERS
210 220 230 240 250
VHHSGAVRNY NRDEVQLPRG PSATPVDCSL CGKKRRYVRL GLSSSSSLSS
260 270 280 290 300
HTAGVTEKHS QDSYNSLSMD IIGDPSQAYT GSEVVNDHQS NKGKKTNFLK
310
KDKSMEWFTG SEE
Length:313
Mass (Da):36,463
Last modified:May 2, 2006 - v2
Checksum:iF511EECB26245EEC
GO
Isoform 11 Publication (identifier: Q5MJ70-1) [UniParc]FASTAAdd to basket
Also known as: A11 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     284-286: VVN → GMI
     287-313: Missing.

Show »
Length:286
Mass (Da):33,269
Checksum:i348BAE62F09024E9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052027284 – 286VVN → GMI in isoform 1. 2 Publications3
Alternative sequenceiVSP_052028287 – 313Missing in isoform 1. 2 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY820303 mRNA. Translation: AAW30394.1.
AY820304 mRNA. Translation: AAW30395.1.
AC097720 Genomic DNA. Translation: AAY24014.1.
BC093005 mRNA. Translation: AAH93005.1.
CCDSiCCDS1767.2. [Q5MJ70-2]
RefSeqiNP_001008779.1. NM_001008779.1. [Q5MJ70-1]
NP_001136106.1. NM_001142634.1. [Q5MJ70-2]
NP_877433.2. NM_182756.3. [Q5MJ70-2]
UniGeneiHs.511956.

Genome annotation databases

EnsembliENST00000334056; ENSP00000335628; ENSG00000163806. [Q5MJ70-2]
ENST00000379579; ENSP00000368898; ENSG00000163806. [Q5MJ70-2]
GeneIDi245711.
KEGGihsa:245711.
UCSCiuc002rmj.4. human. [Q5MJ70-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPDYA_HUMAN
AccessioniPrimary (citable) accession number: Q5MJ70
Secondary accession number(s): Q53R05, Q5MJ69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: December 20, 2017
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families