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Q5MJ70

- SPDYA_HUMAN

UniProt

Q5MJ70 - SPDYA_HUMAN

Protein

Speedy protein A

Gene

SPDYA

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 2 (02 May 2006)
      Previous versions | rss
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    Functioni

    Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2.3 Publications

    GO - Molecular functioni

    1. protein kinase binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB
    2. G1/S transition of mitotic cell cycle Source: UniProtKB
    3. male meiosis Source: UniProtKB
    4. multicellular organismal development Source: UniProtKB-KW
    5. positive regulation of cell proliferation Source: UniProtKB
    6. positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Cell cycle, DNA damage

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Speedy protein A
    Alternative name(s):
    Rapid inducer of G2/M progression in oocytes A
    Short name:
    RINGO A
    Short name:
    hSpy/Ringo A
    Speedy-1
    Short name:
    Spy1
    Gene namesi
    Name:SPDYAImported
    Synonyms:SPDY1Imported, SPY1Imported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:30613. SPDYA.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134954857.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Speedy protein APRO_0000234112Add
    BLAST

    Proteomic databases

    PaxDbiQ5MJ70.
    PRIDEiQ5MJ70.

    PTM databases

    PhosphoSiteiQ5MJ70.

    Expressioni

    Tissue specificityi

    Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus. Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase.2 Publications

    Gene expression databases

    ArrayExpressiQ5MJ70.
    BgeeiQ5MJ70.
    CleanExiHS_SPDYA.
    GenevestigatoriQ5MJ70.

    Interactioni

    Subunit structurei

    Interacts with CDK1, CDK2 and CDKN1B/KIP1. Found in a complex with both CDK2 and CDKN1B/KIP1.3 Publications

    Protein-protein interaction databases

    BioGridi128828. 5 interactions.
    IntActiQ5MJ70. 2 interactions.
    MINTiMINT-7226374.
    STRINGi9606.ENSP00000335628.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5MJ70.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni68 – 200133Speedy/Ringo box; Required for CDK-bindingBy similarityAdd
    BLAST

    Domaini

    The C-terminus is required for CDK2-activation, but not CDK2-binding.By similarity

    Sequence similaritiesi

    Belongs to the Speedy/Ringo family.Curated

    Phylogenomic databases

    eggNOGiNOG42793.
    HOGENOMiHOG000060281.
    HOVERGENiHBG086798.
    InParanoidiQ5MJ70.
    KOiK08694.
    OMAiKHSQESH.
    OrthoDBiEOG7PS1FZ.
    PhylomeDBiQ5MJ70.
    TreeFamiTF329827.

    Family and domain databases

    InterProiIPR020984. Cell_cycle_regulatory_Spy1.
    [Graphical view]
    PfamiPF11357. Spy1. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 22 Publications (identifier: Q5MJ70-2) [UniParc]FASTAAdd to Basket

    Also known as: A21 Publication

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRHNQMCCET PPTVTVYVKS GSNRSHQPKK PITLKRPICK DNWQAFEKNT    50
    HNNNKSKRPK GPCLVIQRQD MTAFFKLFDD DLIQDFLWMD CCCKIADKYL 100
    LAMTFVYFKR AKFTISEHTR INFFIALYLA NTVEEDEEET KYEIFPWALG 150
    KNWRKLFPNF LKLRDQLWDR IDYRAIVSRR CCEEVMAIAP THYIWQRERS 200
    VHHSGAVRNY NRDEVQLPRG PSATPVDCSL CGKKRRYVRL GLSSSSSLSS 250
    HTAGVTEKHS QDSYNSLSMD IIGDPSQAYT GSEVVNDHQS NKGKKTNFLK 300
    KDKSMEWFTG SEE 313
    Length:313
    Mass (Da):36,463
    Last modified:May 2, 2006 - v2
    Checksum:iF511EECB26245EEC
    GO
    Isoform 11 Publication (identifier: Q5MJ70-1) [UniParc]FASTAAdd to Basket

    Also known as: A11 Publication

    The sequence of this isoform differs from the canonical sequence as follows:
         284-286: VVN → GMI
         287-313: Missing.

    Show »
    Length:286
    Mass (Da):33,269
    Checksum:i348BAE62F09024E9
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei284 – 2863VVN → GMI in isoform 1. 2 PublicationsVSP_052027
    Alternative sequencei287 – 31327Missing in isoform 1. 2 PublicationsVSP_052028Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY820303 mRNA. Translation: AAW30394.1.
    AY820304 mRNA. Translation: AAW30395.1.
    AC097720 Genomic DNA. Translation: AAY24014.1.
    BC093005 mRNA. Translation: AAH93005.1.
    CCDSiCCDS1767.2. [Q5MJ70-2]
    RefSeqiNP_001008779.1. NM_001008779.1. [Q5MJ70-1]
    NP_001136106.1. NM_001142634.1. [Q5MJ70-2]
    NP_877433.2. NM_182756.3. [Q5MJ70-2]
    UniGeneiHs.511956.

    Genome annotation databases

    EnsembliENST00000334056; ENSP00000335628; ENSG00000163806. [Q5MJ70-2]
    ENST00000379579; ENSP00000368898; ENSG00000163806. [Q5MJ70-2]
    GeneIDi245711.
    KEGGihsa:245711.
    UCSCiuc002rmi.3. human. [Q5MJ70-1]
    uc002rmj.3. human. [Q5MJ70-2]

    Polymorphism databases

    DMDMi94730574.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY820303 mRNA. Translation: AAW30394.1 .
    AY820304 mRNA. Translation: AAW30395.1 .
    AC097720 Genomic DNA. Translation: AAY24014.1 .
    BC093005 mRNA. Translation: AAH93005.1 .
    CCDSi CCDS1767.2. [Q5MJ70-2 ]
    RefSeqi NP_001008779.1. NM_001008779.1. [Q5MJ70-1 ]
    NP_001136106.1. NM_001142634.1. [Q5MJ70-2 ]
    NP_877433.2. NM_182756.3. [Q5MJ70-2 ]
    UniGenei Hs.511956.

    3D structure databases

    ProteinModelPortali Q5MJ70.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 128828. 5 interactions.
    IntActi Q5MJ70. 2 interactions.
    MINTi MINT-7226374.
    STRINGi 9606.ENSP00000335628.

    PTM databases

    PhosphoSitei Q5MJ70.

    Polymorphism databases

    DMDMi 94730574.

    Proteomic databases

    PaxDbi Q5MJ70.
    PRIDEi Q5MJ70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000334056 ; ENSP00000335628 ; ENSG00000163806 . [Q5MJ70-2 ]
    ENST00000379579 ; ENSP00000368898 ; ENSG00000163806 . [Q5MJ70-2 ]
    GeneIDi 245711.
    KEGGi hsa:245711.
    UCSCi uc002rmi.3. human. [Q5MJ70-1 ]
    uc002rmj.3. human. [Q5MJ70-2 ]

    Organism-specific databases

    CTDi 245711.
    GeneCardsi GC02P029007.
    H-InvDB HIX0021816.
    HGNCi HGNC:30613. SPDYA.
    MIMi 614029. gene.
    neXtProti NX_Q5MJ70.
    PharmGKBi PA134954857.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG42793.
    HOGENOMi HOG000060281.
    HOVERGENi HBG086798.
    InParanoidi Q5MJ70.
    KOi K08694.
    OMAi KHSQESH.
    OrthoDBi EOG7PS1FZ.
    PhylomeDBi Q5MJ70.
    TreeFami TF329827.

    Miscellaneous databases

    ChiTaRSi SPDYA. human.
    GeneWikii SPDYA.
    GenomeRNAii 245711.
    NextBioi 91722.
    PROi Q5MJ70.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q5MJ70.
    Bgeei Q5MJ70.
    CleanExi HS_SPDYA.
    Genevestigatori Q5MJ70.

    Family and domain databases

    InterProi IPR020984. Cell_cycle_regulatory_Spy1.
    [Graphical view ]
    Pfami PF11357. Spy1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human Speedy: a novel cell cycle regulator that enhances proliferation through activation of Cdk2."
      Porter L.A., Dellinger R.W., Tynan J.A., Barnes E.A., Kong M., Lenormand J.-L., Donoghue D.J.
      J. Cell Biol. 157:357-366(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH CDK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Testis1 Publication.
    2. "Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins."
      Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.
      Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
      Tissue: TestisImported.
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: TestisImported.
    5. "Human Spy1 promotes survival of mammalian cells following DNA damage."
      Barnes E.A., Porter L.A., Lenormand J.-L., Dellinger R.W., Donoghue D.J.
      Cancer Res. 63:3701-3707(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDK2.
    6. "Spy1 interacts with p27Kip1 to allow G1/S progression."
      Porter L.A., Kong-Beltran M., Donoghue D.J.
      Mol. Biol. Cell 14:3664-3674(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDKN1B, IDENTIFICATION IN A COMPLEX WITH CDKN1B AND CDK2, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSPDYA_HUMAN
    AccessioniPrimary (citable) accession number: Q5MJ70
    Secondary accession number(s): Q53R05, Q5MJ69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 72 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3