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Q5MFV8

- PME5_ARATH

UniProt

Q5MFV8 - PME5_ARATH

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Protein
Pectinesterase 5
Gene
PME5, ARATH67, VGD1, At2g47040, F14M4.13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin. Plays an important role in growth of pollen tubes in female floral tissues, possibly via enhancing the interaction between the pollen tube and female floral tissues by modification of the cell walls.1 Publication

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei360 – 3601Substrate By similarity
Binding sitei390 – 3901Substrate By similarity
Sitei412 – 4121Transition state stabilizer By similarity
Active sitei413 – 4131Proton donor By similarity
Active sitei434 – 4341Nucleophile By similarity
Binding sitei503 – 5031Substrate By similarity
Binding sitei505 – 5051Substrate By similarity

GO - Molecular functioni

  1. aspartyl esterase activity Source: UniProtKB-KW
  2. enzyme inhibitor activity Source: InterPro
  3. pectinesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall modification Source: InterPro
  2. pectin catabolic process Source: UniProtKB-UniPathway
  3. pollen tube growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G47040-MONOMER.
BRENDAi3.1.1.11. 399.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase 5 (EC:3.1.1.11)
Short name:
PE 5
Alternative name(s):
Pectin methylesterase 5
Short name:
AtPME5
Pectin methylesterase 67
Short name:
AtPME67
Protein VANGUARD 1
Gene namesi
Name:PME5
Synonyms:ARATH67, VGD1
Ordered Locus Names:At2g47040
ORF Names:F14M4.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G47040.

Subcellular locationi

Cell membrane. Secretedcell wall
Note: Distributed in the whole pollen tube, including the plasma membrane and pollen tube wall.1 Publication

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
  4. pollen tube Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Chaini25 – 595571Pectinesterase 5
PRO_0000023477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi86 – 861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi206 – 2061N-linked (GlcNAc...) Reviewed prediction
Glycosylationi349 – 3491N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5MFV8.
PRIDEiQ5MFV8.

Expressioni

Tissue specificityi

Expressed in pollen grains and pollen tubes.1 Publication

Developmental stagei

Expressed throughout silique development.1 Publication

Gene expression databases

GenevestigatoriQ5MFV8.

Interactioni

Protein-protein interaction databases

BioGridi4653. 2 interactions.
STRINGi3702.AT2G47040.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ5MFV8.
SMRiQ5MFV8. Positions 285-595.

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.
In the C-terminal section; belongs to the pectinesterase family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4677.
HOGENOMiHOG000217409.
InParanoidiQ5MFV8.
OMAiTHANILM.
PhylomeDBiQ5MFV8.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR018040. Pectinesterase_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5MFV8-1 [UniParc]FASTAAdd to Basket

« Hide

MIGKVVVSVA SILLIVGVAI GVVAYINKNG DANLSPQMKA VRGICEATSD    50
KASCVKTLEP VKSDDPNKLI KAFMLATRDA ITQSSNFTGK TEGNLGSGIS 100
PNNKAVLDYC KKVFMYALED LSTIVEEMGE DLNQIGSKID QLKQWLTGVY 150
NYQTDCLDDI EEDDLRKTIG EGIASSKILT SNAIDIFHTV VSAMAKLNLK 200
VEDFKNMTGG IFAPSDKGAA PVNKGTPPVA DDSPVADPDG PARRLLEDID 250
ETGIPTWVSG ADRKLMTKAG RGSNDGGARI RATFVVAKDG SGQFKTVQQA 300
VNACPEKNPG RCIIHIKAGI YREQVIIPKK KNNIFMFGDG ARKTVISYNR 350
SVKLSPGTTT SLSGTVQVES EGFMAKWIGF KNTAGPMGHQ AVAIRVNGDR 400
AVIFNCRFDG YQDTLYVNNG RQFYRNIVVS GTVDFIFGKS ATVIQNSLIV 450
VRKGNKGQFN TVTADGNEKG LAMKIGIVLQ NCRIVPDKKL AAERLIVESY 500
LGRPWKKFST TVIINSEIGD VIRPEGWKIW DGESFHKSCR YVEYNNRGPG 550
AITNRRVNWV KIARSAAEVN DFTVANWLGP INWIQEANVP VTLGL 595
Length:595
Mass (Da):64,728
Last modified:March 15, 2005 - v2
Checksum:iE314E53201F3CD77
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501D → V in AAV91508. 1 Publication
Sequence conflicti93 – 931G → E in CAB58974. 1 Publication
Sequence conflicti138 – 1381K → E in AAV91508. 1 Publication
Sequence conflicti267 – 2671T → A1 Publication
Sequence conflicti267 – 2671T → A1 Publication
Sequence conflicti537 – 5371K → Q in AAV91508. 1 Publication
Sequence conflicti545 – 5451N → S in AAV91508. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY830948 mRNA. Translation: AAV91508.1.
AJ250430 mRNA. Translation: CAB58974.1.
AC004411 Genomic DNA. Translation: AAC34240.1.
CP002685 Genomic DNA. Translation: AEC10791.1.
AY091768 mRNA. Translation: AAM10316.1.
BT001120 mRNA. Translation: AAN64511.1.
PIRiT02183.
T52327.
RefSeqiNP_182227.1. NM_130272.3.
UniGeneiAt.24875.

Genome annotation databases

EnsemblPlantsiAT2G47040.1; AT2G47040.1; AT2G47040.
GeneIDi819318.
KEGGiath:AT2G47040.

Cross-referencesi

Web resourcesi

Protein Spotlight

When tough is soft - Issue 106 of June 2009

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY830948 mRNA. Translation: AAV91508.1 .
AJ250430 mRNA. Translation: CAB58974.1 .
AC004411 Genomic DNA. Translation: AAC34240.1 .
CP002685 Genomic DNA. Translation: AEC10791.1 .
AY091768 mRNA. Translation: AAM10316.1 .
BT001120 mRNA. Translation: AAN64511.1 .
PIRi T02183.
T52327.
RefSeqi NP_182227.1. NM_130272.3.
UniGenei At.24875.

3D structure databases

ProteinModelPortali Q5MFV8.
SMRi Q5MFV8. Positions 285-595.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4653. 2 interactions.
STRINGi 3702.AT2G47040.1-P.

Proteomic databases

PaxDbi Q5MFV8.
PRIDEi Q5MFV8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G47040.1 ; AT2G47040.1 ; AT2G47040 .
GeneIDi 819318.
KEGGi ath:AT2G47040.

Organism-specific databases

GeneFarmi 443. 8.
TAIRi AT2G47040.

Phylogenomic databases

eggNOGi COG4677.
HOGENOMi HOG000217409.
InParanoidi Q5MFV8.
OMAi THANILM.
PhylomeDBi Q5MFV8.

Enzyme and pathway databases

UniPathwayi UPA00545 ; UER00823 .
BioCyci ARA:AT2G47040-MONOMER.
BRENDAi 3.1.1.11. 399.

Gene expression databases

Genevestigatori Q5MFV8.

Family and domain databases

Gene3Di 1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProi IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR018040. Pectinesterase_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
[Graphical view ]
Pfami PF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view ]
SMARTi SM00856. PMEI. 1 hit.
[Graphical view ]
SUPFAMi SSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsi TIGR01614. PME_inhib. 1 hit.
PROSITEi PS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "VANGUARD1 encodes a pectin methylesterase that enhances pollen tube growth in the Arabidopsis style and transmitting tract."
    Jiang L., Yang S.-L., Xie L.-F., Puah C.S., Zhang X.-Q., Yang W.-C., Sundaresan V., Ye D.
    Plant Cell 17:584-596(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: cv. Landsberg erecta.
  2. "Characterization of a flower-specific gene encoding pectin methylesterase in Arabidopsis thaliana."
    Torki M., Mache R., Mandaron P., Falconet D.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Flower bud.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Pectin methylesterases: sequence-structural features and phylogenetic relationships."
    Markovic O., Janecek S.
    Carbohydr. Res. 339:2281-2295(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana."
    Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J.
    Planta 224:782-791(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPME5_ARATH
AccessioniPrimary (citable) accession number: Q5MFV8
Secondary accession number(s): O80721, Q9SMV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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