Q5MFV5 (P2C34_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 34
Short name=OsPP2C34
EC=3.1.3.16

Alternative name(s):
BTH-induced protein phosphatase 2C 2
Short name=OsBIPP2C2

Gene names

Name:	BIPP2C2
ORF Names:	OsI_013174

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Induction	By benzothiadiazole (BTH). Ref.1
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5MFV5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5MFV5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     288-317: TGIANRLVKAALKEATRKREVSFRDLKTIE → TVSSDNIYSIRSFHHREYIFPVNERGVLTQ
     318-380: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q5MFV5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     233-255: VGPPIPLKRPALSAEPSIQVRKL → MVSGSISVTTLRCRLSSRIQGRV
     256-380: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Probable protein phosphatase 2C 34		PRO_0000363280
Regions
	Domain	26 – 307	282	PP2C-like
Sites
	Metal binding	66	1	Manganese 1 By similarity
	Metal binding	66	1	Manganese 2 By similarity
	Metal binding	67	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	267	1	Manganese 2 By similarity
	Metal binding	326	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	233 – 255	23	VGPPI…QVRKL → MVSGSISVTTLRCRLSSRIQ GRV in isoform 3.		VSP_036259
	Alternative sequence	256 – 380	125	Missing in isoform 3.		VSP_036260
	Alternative sequence	288 – 317	30	TGIAN…LKTIE → TVSSDNIYSIRSFHHREYIF PVNERGVLTQ in isoform 2.		VSP_036261
	Alternative sequence	318 – 380	63	Missing in isoform 2.		VSP_036262
Experimental info
	Sequence conflict	84	1	H → L in AAW29521. Ref.1
	Sequence conflict	84	1	H → L in AAW29522. Ref.1
	Sequence conflict	175	1	A → T in AAW29521. Ref.1
	Sequence conflict	175	1	A → T in AAW29522. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: A52FA669C224987F	FASTA	380	41,754
	10 20 30 40 50 60 MLRAVARCCG HWPPGAAAAD GMLWQTELRP HAAGEFSMAA AQANLAMEDQ AQVLASPAAT 70 80 90 100 110 120 LVGVYDGHGG ADASRFLRSR LFPHVQRFEK EQGGMSTEVI RRAFGAAEEE FLQQVRQAWR 130 140 150 160 170 180 QRPKMAAVGS CCLLGAISGD TLYVANLGDS RAVLGRRVVG GGVAVAERLT DEHNAASEEV 190 200 210 220 230 240 RRELTALNPD DAQIVVHARG AWRVKGIIQV SRTIGDVYLK KQEYSMDPVF RNVGPPIPLK 250 260 270 280 290 300 RPALSAEPSI QVRKLKPNDL FLIFASDGLW EHLSDDAAVQ IVFKNPRTGI ANRLVKAALK 310 320 330 340 350 360 EATRKREVSF RDLKTIEKGV RRHFHDDISV IVVYLDRHRG RRHTRVVDSS SNCTNAPVDI 370 380 YSSNSGQSVE TLQAHRGSGW
	Isoform 2 [UniParc]. Checksum: B3765511788DEA42	FASTA	317	34,806
	10 20 30 40 50 60 MLRAVARCCG HWPPGAAAAD GMLWQTELRP HAAGEFSMAA AQANLAMEDQ AQVLASPAAT 70 80 90 100 110 120 LVGVYDGHGG ADASRFLRSR LFPHVQRFEK EQGGMSTEVI RRAFGAAEEE FLQQVRQAWR 130 140 150 160 170 180 QRPKMAAVGS CCLLGAISGD TLYVANLGDS RAVLGRRVVG GGVAVAERLT DEHNAASEEV 190 200 210 220 230 240 RRELTALNPD DAQIVVHARG AWRVKGIIQV SRTIGDVYLK KQEYSMDPVF RNVGPPIPLK 250 260 270 280 290 300 RPALSAEPSI QVRKLKPNDL FLIFASDGLW EHLSDDAAVQ IVFKNPRTVS SDNIYSIRSF 310 HHREYIFPVN ERGVLTQ
	Isoform 3 [UniParc]. Checksum: 9F404754981AA892	FASTA	255	27,659
	10 20 30 40 50 60 MLRAVARCCG HWPPGAAAAD GMLWQTELRP HAAGEFSMAA AQANLAMEDQ AQVLASPAAT 70 80 90 100 110 120 LVGVYDGHGG ADASRFLRSR LFPHVQRFEK EQGGMSTEVI RRAFGAAEEE FLQQVRQAWR 130 140 150 160 170 180 QRPKMAAVGS CCLLGAISGD TLYVANLGDS RAVLGRRVVG GGVAVAERLT DEHNAASEEV 190 200 210 220 230 240 RRELTALNPD DAQIVVHARG AWRVKGIIQV SRTIGDVYLK KQEYSMDPVF RNMVSGSISV 250 TTLRCRLSSR IQGRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of four rice genes encoding ethylene-responsive transcriptional factors and their expressions in response to biotic and abiotic stress." Cao Y., Song F., Goodman R.M., Zheng Z. J. Plant Physiol. 163:1167-1178(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), INDUCTION. Strain: cv. Yuanfengzao. Tissue: Seedling.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.
[3]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY831390 mRNA. Translation: AAW29521.1. AY831391 mRNA. Translation: AAW29522.1. CM000128 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	Q5MFV5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q5MFV5.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000238703.
Gene expression databases
ArrayExpress	Q5MFV5.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C34_ORYSI

Accession

Primary (citable) accession number: Q5MFV5
Secondary accession number(s): A2XM81, Q5MFV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	April 3, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program