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Q5MD89 (VGFR3_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vascular endothelial growth factor receptor 3
Short name=VEGFR-3
EC=2.7.10.1
Gene names
Name:flt4
Synonyms:flt-4, vegfr3
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length1357 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for vegf or vegfc. Combinations of multiple VEGF receptors are required for development of different blood vessel types in the embryo. Involved in angiogenesis, specifically in VEGF-induced sprouting of new blood vessels, but not required for proper vasculogenesis or hematopoiesis. Ref.2

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation

Present in an inactive conformation in the absence of bound ligand. Binding of vegfc or vegfd leads to dimerization and activation by autophosphorylation on tyrosine residues By similarity.

Subunit structure

Interacts with vegfc and vegfd. Monomer in the absence of bound vegfc or vegfd. Homodimer in the presence of bound vegfc or vegfd By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Note: Ligand-mediated autophosphorylation leads to rapid internalization By similarity.

Post-translational modification

Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 13571333Vascular endothelial growth factor receptor 3
PRO_0000249464

Regions

Topological domain25 – 796772Extracellular Potential
Transmembrane797 – 81721Helical; Potential
Topological domain818 – 1357540Cytoplasmic Potential
Domain25 – 12197Ig-like C2-type 1
Domain138 – 244107Ig-like C2-type 2
Domain255 – 34389Ig-like C2-type 3
Domain352 – 44291Ig-like C2-type 4
Domain453 – 583131Ig-like C2-type 5
Domain583 – 690108Ig-like C2-type 6
Domain699 – 78587Ig-like C2-type 7
Domain866 – 1181316Protein kinase
Nucleotide binding872 – 8809ATP By similarity

Sites

Active site10451Proton acceptor By similarity
Binding site9001ATP By similarity

Amino acid modifications

Modified residue10711Phosphotyrosine; by autocatalysis By similarity
Modified residue10761Phosphotyrosine; by autocatalysis By similarity
Modified residue12261Phosphotyrosine; by autocatalysis By similarity
Modified residue12271Phosphotyrosine; by autocatalysis By similarity
Modified residue13341Phosphotyrosine; by autocatalysis By similarity
Modified residue13381Phosphotyrosine; by autocatalysis By similarity
Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation2161N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation3601N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential
Glycosylation4401N-linked (GlcNAc...) Potential
Glycosylation5531N-linked (GlcNAc...) Potential
Glycosylation6101N-linked (GlcNAc...) Potential
Glycosylation6601N-linked (GlcNAc...) Potential
Glycosylation7071N-linked (GlcNAc...) Potential
Glycosylation7111N-linked (GlcNAc...) Potential
Glycosylation7511N-linked (GlcNAc...) Potential
Disulfide bond51 ↔ 121 By similarity
Disulfide bond173 ↔ 225 By similarity
Disulfide bond272 ↔ 331 By similarity
Disulfide bond473 ↔ 562 By similarity
Disulfide bond606 ↔ 674 By similarity
Disulfide bond720 ↔ 772 By similarity

Experimental info

Sequence conflict5671R → K in AAS92272. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5MD89 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: D68C197828BE278E

FASTA1,357153,178
        10         20         30         40         50         60 
MKRDFTFFCR IWIGIPFFSG LVNGFSMSPP TLDNTKDQLV INANDTLNIT CRGQRILDWS 

        70         80         90        100        110        120 
WPEESLSKVE FTDRQGQQSP TDTPGYREIR LKECQGVAGK PYCKILILTN AQANDSGYYR 

       130        140        150        160        170        180 
CFYKDIKAVI DGTTAASIFV FVRDPEHPFI KRGDNDMETI FITDSETHIE VPCLVSDPDL 

       190        200        210        220        230        240 
KVTLFSLVPY PEPVDGSVVT WNNKKGWSIP RHIIQNTSTF IGFYCSISVQ NSQHTSSIYV 

       250        260        270        280        290        300 
VQVIGLKFYE FKLFPEDSPV ELMQGESLVL NCTALVDFNT GVDFQWDYPG KKENRLASLQ 

       310        320        330        340        350        360 
PLRNVLDEAT EISSILSIRN IHLDDSGYYT CWANTLEMKR ELTTVVIVHE KPFISLDYRN 

       370        380        390        400        410        420 
GSVIEAKEGQ KSVRLSVKVS AYPSPEIQWY KNGKLISSKN SSRFKVQQHS LQIRDVCKQD 

       430        440        450        460        470        480 
AGEYMLVLKN SPAALEKRLN FTLIVNVPPQ IHEKEAAPPT NLYGKGTRQI LTCTADGSPP 

       490        500        510        520        530        540 
ASISWQWRPW SPCDLERTRR ALRRRGGRDQ SPFCHNWMDL DPEHAVNPIE SIDTLTQMVD 

       550        560        570        580        590        600 
GKEKTVGRVV IQNASVPAMY KCLAENRVGK DERLIYFYVT TIPEGFDIEM EPSEDPLEQD 

       610        620        630        640        650        660 
LVQLKCNADN FTYENLRWYR LDPQTVPPEL DCKSLHQYAT FLEGQLSFQT TSNNWVLQLN 

       670        680        690        700        710        720 
ITNIQLQDEG NYVCEVQNRR TGVKHCHRKY IPVKAMEAPR YRHNPTNHTV NVSESLQMNC 

       730        740        750        760        770        780 
DVEGTPFPQL SWFKDNQPLH QISGILLQDS NRTLSIQRVR EEDAGLYTCS ACNQKGCVQS 

       790        800        810        820        830        840 
SATVSVIGSD DKTNVEIVIL IGTGVIAIFF WVLLLVIFCN VKRVNPADIK TGYLSIIMDP 

       850        860        870        880        890        900 
GEVPLEEQCE YLPYDSSQWE ISRDRLRLGK VLGHGAFGKV IEASIFGHDK KSSANTVAVK 

       910        920        930        940        950        960 
MLKEGATASE HKALMSELKI LIHIGNHLNV VNLLGACTKP NGPLMVIVEY CKYGNLSNFL 

       970        980        990       1000       1010       1020 
RAKREFFLPY RDRSPKTQSQ VRRMIEAGQA SQSEHQPSTS STNPPRVTVD DLWKTPLTIE 

      1030       1040       1050       1060       1070       1080 
DLICYSFQVA RGMEFLASRK CIHRDLAARN ILLSENNVVK ICDFGLARDI YKDPDYVRKG 

      1090       1100       1110       1120       1130       1140 
NARLPLKWMA PESIFDKVYT SQSDVWSFGV LLWEIFSLGA SPYPGIQIDE DFCKRLKDGT 

      1150       1160       1170       1180       1190       1200 
RMRAPDNASP EIYGIMLACW QGEPRERPTF PALVEILGDL LQENSLPEIP FNVSQSSEDD 

      1210       1220       1230       1240       1250       1260 
GFSQASSRPP SQEEIRLACN TLPTRYYNCV PFAGCVMVGP SSTCHSRVKT FEELPMEMTS 

      1270       1280       1290       1300       1310       1320 
HKTQHDSQTD SGMVLASDEL ERFEHKHRGA MLTTATTGQS TDRLISCPSV SSSGSGGGLL 

      1330       1340       1350 
RPVFFTQLSG QTFYNNEYGH LSEEGVSDYF SSSDQAV

« Hide

References

[1]"Synergistic signaling of vegf receptors is required for vasculogenesis in zebrafish."
Habeck H., Langhoff J., Vogel A.M., Trowe T., Koblizek T.I., Schulte-Merker S.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Distinct genetic interactions between multiple Vegf receptors are required for development of different blood vessel types in zebrafish."
Covassin L.D., Villefranc J.A., Kacergis M.C., Weinstein B.M., Lawson N.D.
Proc. Natl. Acad. Sci. U.S.A. 103:6554-6559(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY524001 mRNA. Translation: AAS92272.1.
AY833404 mRNA. Translation: AAV93318.1.
IPIIPI00491968.
RefSeqNP_571020.1. NM_130945.1.
UniGeneDr.81298.

3D structure databases

ProteinModelPortalQ5MD89.
SMRQ5MD89. Positions 848-1185.
ModBaseSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000005480.

Proteomic databases

PRIDEQ5MD89.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID30121.
KEGGdre:30121.

Organism-specific databases

CTD2324.
ZFINZDB-GENE-980526-326. flt4.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000037949.
HOVERGENHBG053432.
InParanoidQ5GIT2.
KOK05097.
OrthoDBEOG49W2DJ.

Family and domain databases

Gene3D2.60.40.10. 7 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001824. Tyr_kinase_rcpt_3_CS.
IPR009137. Tyr_kinase_VEGFR3_rcpt_N.
IPR009134. Tyr_kinase_VEGFR_rcpt_N.
[Graphical view]
PfamPF07679. I-set. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSPR01832. VEGFRECEPTOR.
PR01835. VEGFRECEPTR3.
SMARTSM00409. IG. 3 hits.
SM00408. IGc2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS50835. IG_LIKE. 5 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806600.

Entry information

Entry nameVGFR3_DANRE
AccessionPrimary (citable) accession number: Q5MD89
Secondary accession number(s): Q5GIT2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: February 1, 2005
Last modified: May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents