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Protein

Protein HEXIM1

Gene

Hexim1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processImmunity, Innate immunity, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HEXIM1
Alternative name(s):
Cardiac lineage protein 1
Gene namesi
Name:Hexim1
Synonyms:Clp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1559851 Hexim1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003052651 – 356Protein HEXIM1Add BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei98PhosphoserineBy similarity1
Modified residuei103PhosphoserineCombined sources1
Modified residuei230PhosphoserineBy similarity1
Modified residuei233PhosphothreonineBy similarity1
Modified residuei234PhosphoserineBy similarity1
Modified residuei249PhosphoserineCombined sources1
Modified residuei257PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5M9G1
PRIDEiQ5M9G1

PTM databases

iPTMnetiQ5M9G1
PhosphoSitePlusiQ5M9G1

Expressioni

Inductioni

Up-regulated by HMBA (hexamethylene bisacetamide).1 Publication

Gene expression databases

BgeeiENSRNOG00000003203
GenevisibleiQ5M9G1 RN

Interactioni

Subunit structurei

Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Interacts with CCNT2; mediates formation of a tripartite complex with KPNA2. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 non-coding RNA.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004272

Structurei

3D structure databases

ProteinModelPortaliQ5M9G1
SMRiQ5M9G1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 174Basic region; mediates nuclear localization and interaction with 7SK snRNA and NR3C1By similarityAdd BLAST28
Regioni199 – 202Interaction with P-TEFbBy similarity4
Regioni207 – 247Autoinhibitory acidic region; in absence of 7SK snRNA interacts with the basic region preventing interaction with P-TEFb and modulating subcellular localizationBy similarityAdd BLAST41
Regioni283 – 311Mediates interaction with CCNT1By similarityAdd BLAST29
Regioni307 – 352Required for inhibition of ESR1-dependent transcriptionBy similarityAdd BLAST46

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili280 – 346Sequence analysisAdd BLAST67

Domaini

The coiled-coil domain mediates oligomerization.By similarity

Sequence similaritiesi

Belongs to the HEXIM family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IH8I Eukaryota
ENOG4111K9Z LUCA
GeneTreeiENSGT00390000002808
HOGENOMiHOG000060338
HOVERGENiHBG053249
InParanoidiQ5M9G1
KOiK15189
OMAiEAWGQQQ
OrthoDBiEOG091G0Y9A
PhylomeDBiQ5M9G1
TreeFamiTF336851

Family and domain databases

InterProiView protein in InterPro
IPR024872 HEXIM
PANTHERiPTHR13469 PTHR13469, 1 hit
PfamiView protein in Pfam
PF15313 HEXIM, 1 hit
PRINTSiPR02094 HEXIMFAMILY

Sequencei

Sequence statusi: Complete.

Q5M9G1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPLLSEHQ HQPQTSNCTG AAVVHEEQNS ERPPSAEERV PKEDSRWQSR
60 70 80 90 100
ASLQSGSRPG QEGDGGLKHQ LPPLQTNACP ELSCLEKGEK GQNGEDLSTG
110 120 130 140 150
GASPSAEGEP MSESLVQPGH DSEATKLEAP VAGVEEPWGQ QQRQLGKKKH
160 170 180 190 200
RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRVRAEM FAKGQPVAPY
210 220 230 240 250
NTTQFLMDDH DQEEPDLKTG LYPKRAAAKS DDTSDEDFVE EAGEEDGGSD
260 270 280 290 300
GMGGDGSEFL QRDFSETYER YHAESLQNMS KQELIKEYLE LEKCLSRKED
310 320 330 340 350
ENNRLRLESK RLGGVDARVR ELELELDRLR AENRQLLTEN ELHRQQERAP

PSKFGD
Length:356
Mass (Da):40,317
Last modified:February 1, 2005 - v1
Checksum:i7854474F7E628E6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087133 mRNA Translation: AAH87133.1
RefSeqiNP_001020307.1, NM_001025136.1
UniGeneiRn.113703

Genome annotation databases

EnsembliENSRNOT00000004272; ENSRNOP00000004272; ENSRNOG00000003203
GeneIDi498008
KEGGirno:498008
UCSCiRGD:1559851 rat

Similar proteinsi

Entry informationi

Entry nameiHEXI1_RAT
AccessioniPrimary (citable) accession number: Q5M9G1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health