Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable tRNA(His) guanylyltransferase

Gene

Thg1l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis (By similarity).By similarity

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Magnesium 1; catalyticBy similarity
Metal bindingi58 – 581Magnesium 2; catalyticBy similarity
Metal bindingi59 – 591Magnesium 1; via carbonyl oxygen; catalyticBy similarity
Metal bindingi105 – 1051Magnesium 1; catalyticBy similarity
Metal bindingi105 – 1051Magnesium 2; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi58 – 636GTPBy similarity
Nucleotide bindingi104 – 1052GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA(His) guanylyltransferase (EC:2.7.7.79)
Alternative name(s):
tRNA-histidine guanylyltransferase
Gene namesi
Name:Thg1l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1359513. Thg1l.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Probable tRNA(His) guanylyltransferasePRO_0000284986Add
BLAST

Proteomic databases

PaxDbiQ5M965.
PRIDEiQ5M965.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005539.
GenevisibleiQ5M965. RN.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007917.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2721. Eukaryota.
COG4021. LUCA.
GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
HOVERGENiHBG054289.
InParanoidiQ5M965.
KOiK10761.
OMAiRFEYVKS.
OrthoDBiEOG091G0GYS.
PhylomeDBiQ5M965.
TreeFamiTF325119.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5M965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAFSTARIG SPLAATSVTL RRCLRLGVAM AKSKFEYVRD FEVDDTCLPH
60 70 80 90 100
CWVVVRLDGR NFHRFAEKHN FAKPNDSRAL HLMTKCAQTV MQELEDIVIA
110 120 130 140 150
YGQSDEYSFV FRKRSNWFKR RASKFMTLVA SQFASSYVFY WRDYFEDQPL
160 170 180 190 200
LYPPGFDGRV VLYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT
210 220 230 240 250
PVQAQQRLKG TLTADKNEIL FSEFHINYNN EPHMYRKGTV LVWQKVNEVR
260 270 280 290
TQEIRLPAEM EGEKMAVTRT RTKLVALNCD LIGDAFWKEH PEILEGED
Length:298
Mass (Da):34,850
Last modified:February 1, 2005 - v1
Checksum:i6822E64142F9AE02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087596 mRNA. Translation: AAH87596.1.
RefSeqiNP_001013988.1. NM_001013966.1.
UniGeneiRn.160832.

Genome annotation databases

EnsembliENSRNOT00000007917; ENSRNOP00000007917; ENSRNOG00000005539.
GeneIDi303067.
KEGGirno:303067.
UCSCiRGD:1359513. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087596 mRNA. Translation: AAH87596.1.
RefSeqiNP_001013988.1. NM_001013966.1.
UniGeneiRn.160832.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007917.

Proteomic databases

PaxDbiQ5M965.
PRIDEiQ5M965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007917; ENSRNOP00000007917; ENSRNOG00000005539.
GeneIDi303067.
KEGGirno:303067.
UCSCiRGD:1359513. rat.

Organism-specific databases

CTDi54974.
RGDi1359513. Thg1l.

Phylogenomic databases

eggNOGiKOG2721. Eukaryota.
COG4021. LUCA.
GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
HOVERGENiHBG054289.
InParanoidiQ5M965.
KOiK10761.
OMAiRFEYVKS.
OrthoDBiEOG091G0GYS.
PhylomeDBiQ5M965.
TreeFamiTF325119.

Miscellaneous databases

PROiQ5M965.

Gene expression databases

BgeeiENSRNOG00000005539.
GenevisibleiQ5M965. RN.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHG1_RAT
AccessioniPrimary (citable) accession number: Q5M965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.