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Protein

Probable tRNA(His) guanylyltransferase

Gene

Thg1l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis (By similarity).By similarity

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58Magnesium 1; catalyticBy similarity1
Metal bindingi58Magnesium 2; catalyticBy similarity1
Metal bindingi59Magnesium 1; via carbonyl oxygen; catalyticBy similarity1
Metal bindingi105Magnesium 1; catalyticBy similarity1
Metal bindingi105Magnesium 2; catalyticBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 63GTPBy similarity6
Nucleotide bindingi104 – 105GTPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processtRNA processing
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA(His) guanylyltransferase (EC:2.7.7.79)
Alternative name(s):
tRNA-histidine guanylyltransferase
Gene namesi
Name:Thg1l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1359513 Thg1l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002849861 – 298Probable tRNA(His) guanylyltransferaseAdd BLAST298

Proteomic databases

PaxDbiQ5M965
PRIDEiQ5M965

Expressioni

Gene expression databases

BgeeiENSRNOG00000005539
GenevisibleiQ5M965 RN

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007917

Structurei

3D structure databases

SMRiQ5M965
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2721 Eukaryota
COG4021 LUCA
GeneTreeiENSGT00390000011705
HOGENOMiHOG000204890
HOVERGENiHBG054289
InParanoidiQ5M965
KOiK10761
OMAiNTAFWKL
OrthoDBiEOG091G0GYS
PhylomeDBiQ5M965
TreeFamiTF325119

Family and domain databases

Gene3Di3.30.70.3000, 1 hit
InterProiView protein in InterPro
IPR025845 Thg1_C_dom
IPR024956 tRNAHis_GuaTrfase_cat
IPR007537 tRNAHis_GuaTrfase_Thg1
IPR038469 tRNAHis_GuaTrfase_Thg1_sf
PANTHERiPTHR12729 PTHR12729, 1 hit
PfamiView protein in Pfam
PF04446 Thg1, 1 hit
PF14413 Thg1C, 1 hit
PIRSFiPIRSF028980 tRNAHis_guanylyltransferase, 1 hit

Sequencei

Sequence statusi: Complete.

Q5M965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAFSTARIG SPLAATSVTL RRCLRLGVAM AKSKFEYVRD FEVDDTCLPH
60 70 80 90 100
CWVVVRLDGR NFHRFAEKHN FAKPNDSRAL HLMTKCAQTV MQELEDIVIA
110 120 130 140 150
YGQSDEYSFV FRKRSNWFKR RASKFMTLVA SQFASSYVFY WRDYFEDQPL
160 170 180 190 200
LYPPGFDGRV VLYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT
210 220 230 240 250
PVQAQQRLKG TLTADKNEIL FSEFHINYNN EPHMYRKGTV LVWQKVNEVR
260 270 280 290
TQEIRLPAEM EGEKMAVTRT RTKLVALNCD LIGDAFWKEH PEILEGED
Length:298
Mass (Da):34,850
Last modified:February 1, 2005 - v1
Checksum:i6822E64142F9AE02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087596 mRNA Translation: AAH87596.1
RefSeqiNP_001013988.1, NM_001013966.1
UniGeneiRn.160832

Genome annotation databases

EnsembliENSRNOT00000007917; ENSRNOP00000007917; ENSRNOG00000005539
GeneIDi303067
KEGGirno:303067
UCSCiRGD:1359513 rat

Similar proteinsi

Entry informationi

Entry nameiTHG1_RAT
AccessioniPrimary (citable) accession number: Q5M965
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 1, 2005
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health