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Protein

tRNA-splicing endonuclease subunit Sen2

Gene

Tsen2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei367By similarity1
Active sitei375By similarity1
Active sitei414By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processmRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2
Gene namesi
Name:Tsen2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1309946. Tsen2.

Subcellular locationi

  • Nucleus By similarity
  • Nucleusnucleolus By similarity

  • Note: May be transiently localized in the nucleolus.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094541 – 463tRNA-splicing endonuclease subunit Sen2Add BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineCombined sources1
Modified residuei147PhosphoserineCombined sources1
Modified residuei406PhosphoserineBy similarity1
Modified residuei409PhosphoserineBy similarity1
Modified residuei413PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5M954.
PRIDEiQ5M954.

PTM databases

iPTMnetiQ5M954.
PhosphoSitePlusiQ5M954.

Expressioni

Gene expression databases

BgeeiENSRNOG00000060175.
GenevisibleiQ5M954. RN.

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053267.

Structurei

3D structure databases

ProteinModelPortaliQ5M954.
SMRiQ5M954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiKOG4685. Eukaryota.
COG1676. LUCA.
GeneTreeiENSGT00390000013266.
HOGENOMiHOG000154285.
HOVERGENiHBG056610.
InParanoidiQ5M954.
KOiK15322.
OMAiTFEETFF.
OrthoDBiEOG091G0FMD.
PhylomeDBiQ5M954.

Family and domain databases

InterProiView protein in InterPro
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016589. tRNA_splic_SEN2.
PANTHERiPTHR21227. PTHR21227. 1 hit.
PfamiView protein in Pfam
PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5M954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAVFRAPK RKRRVYESYE SPLPIPFSQD QSPRKEFRIF QAEMISNNVV
60 70 80 90 100
VRGTEDMEQL YGKGYFGKGI LSRSRPNFTI SNPKLAARWK GVQTDMPIIT
110 120 130 140 150
SEKYQHRVEW ARDFMRRQGH DESTVQKILT DYTEPLEPPY RERKGESPQH
160 170 180 190 200
EPLSSKADSS LEGREGKDEL SVTTGGAGQS DDLQGLNTHS DCRQEGPGHA
210 220 230 240 250
TLTVASPSSL NGHAIEDPEA LSQIPCCSQE ALGQQDDLWP EASSQIAGES
260 270 280 290 300
RAAHEYVLIE EELCDVQEGA APHDELLKRK RLVCRRNPYR IFEYLQLSLE
310 320 330 340 350
EAFFLAYALG CLSIYYEKEP LTIVKLWQAF TAVQPTFRTT YMAYHYFRSK
360 370 380 390 400
GWVPKVGLKY GTDLLLYRKG PPFYHASYSV IIELVDDNFE GSLRRPFSWK
410 420 430 440 450
SLAALSRVSG NVSKELMLCY LIKPSTMTNE DMETPECMRR IQVQEVILSR
460
WVSSRERSDQ DEL
Length:463
Mass (Da):52,952
Last modified:February 1, 2005 - v1
Checksum:iF66CD72240AB7229
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087631 mRNA. Translation: AAH87631.1.
RefSeqiNP_001014079.1. NM_001014057.1.
XP_008761462.1. XM_008763240.2.
XP_008761463.1. XM_008763241.1.
XP_008761464.1. XM_008763242.2.
UniGeneiRn.206216.

Genome annotation databases

EnsembliENSRNOT00000081957; ENSRNOP00000073692; ENSRNOG00000060175.
GeneIDi312649.
KEGGirno:312649.
UCSCiRGD:1309946. rat.

Similar proteinsi

Entry informationi

Entry nameiSEN2_RAT
AccessioniPrimary (citable) accession number: Q5M954
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: February 1, 2005
Last modified: August 30, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families