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Q5M954 (SEN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease subunit Sen2

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease Sen2
Gene names
Name:Tsen2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Subunit structure

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 By similarity.

Subcellular location

Nucleus By similarity. Nucleusnucleolus By similarity. Note: May be transiently localized in the nucleolus By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 463463tRNA-splicing endonuclease subunit Sen2
PRO_0000109454

Sites

Active site3671 By similarity
Active site3751 By similarity
Active site4141 By similarity

Amino acid modifications

Modified residue4061Phosphoserine By similarity
Modified residue4091Phosphoserine By similarity
Modified residue4131Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5M954 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: F66CD72240AB7229

FASTA46352,952
        10         20         30         40         50         60 
MAEAVFRAPK RKRRVYESYE SPLPIPFSQD QSPRKEFRIF QAEMISNNVV VRGTEDMEQL 

        70         80         90        100        110        120 
YGKGYFGKGI LSRSRPNFTI SNPKLAARWK GVQTDMPIIT SEKYQHRVEW ARDFMRRQGH 

       130        140        150        160        170        180 
DESTVQKILT DYTEPLEPPY RERKGESPQH EPLSSKADSS LEGREGKDEL SVTTGGAGQS 

       190        200        210        220        230        240 
DDLQGLNTHS DCRQEGPGHA TLTVASPSSL NGHAIEDPEA LSQIPCCSQE ALGQQDDLWP 

       250        260        270        280        290        300 
EASSQIAGES RAAHEYVLIE EELCDVQEGA APHDELLKRK RLVCRRNPYR IFEYLQLSLE 

       310        320        330        340        350        360 
EAFFLAYALG CLSIYYEKEP LTIVKLWQAF TAVQPTFRTT YMAYHYFRSK GWVPKVGLKY 

       370        380        390        400        410        420 
GTDLLLYRKG PPFYHASYSV IIELVDDNFE GSLRRPFSWK SLAALSRVSG NVSKELMLCY 

       430        440        450        460 
LIKPSTMTNE DMETPECMRR IQVQEVILSR WVSSRERSDQ DEL 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC087631 mRNA. Translation: AAH87631.1.
RefSeqNP_001014079.1. NM_001014057.1.
UniGeneRn.206216.

3D structure databases

ProteinModelPortalQ5M954.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000053267.

PTM databases

PhosphoSiteQ5M954.

Proteomic databases

PRIDEQ5M954.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000056429; ENSRNOP00000053267; ENSRNOG00000027158.
GeneID312649.
KEGGrno:312649.
UCSCRGD:1309946. rat.

Organism-specific databases

CTD80746.
RGD1309946. Tsen2.

Phylogenomic databases

eggNOGCOG1676.
GeneTreeENSGT00390000013266.
HOGENOMHOG000154285.
HOVERGENHBG056610.
KOK15322.
OMAKLVCRRN.
OrthoDBEOG76X623.
PhylomeDBQ5M954.

Gene expression databases

GenevestigatorQ5M954.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR016589. tRNA_splic_SEN2.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFPIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
ProtoNetSearch...

Other

NextBio665062.
PROQ5M954.

Entry information

Entry nameSEN2_RAT
AccessionPrimary (citable) accession number: Q5M954
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families