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Q5M954

- SEN2_RAT

UniProt

Q5M954 - SEN2_RAT

Protein

tRNA-splicing endonuclease subunit Sen2

Gene

Tsen2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei367 – 3671By similarity
    Active sitei375 – 3751By similarity
    Active sitei414 – 4141By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    mRNA processing, tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit Sen2 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease Sen2
    Gene namesi
    Name:Tsen2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi1309946. Tsen2.

    Subcellular locationi

    Nucleus By similarity. Nucleusnucleolus By similarity
    Note: May be transiently localized in the nucleolus.By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. cytoplasm Source: Ensembl
    3. nucleolus Source: UniProtKB-SubCell
    4. tRNA-intron endonuclease complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463tRNA-splicing endonuclease subunit Sen2PRO_0000109454Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei406 – 4061PhosphoserineBy similarity
    Modified residuei409 – 4091PhosphoserineBy similarity
    Modified residuei413 – 4131PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ5M954.

    PTM databases

    PhosphoSiteiQ5M954.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5M954.

    Interactioni

    Subunit structurei

    tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000053267.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5M954.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    GeneTreeiENSGT00390000013266.
    HOGENOMiHOG000154285.
    HOVERGENiHBG056610.
    KOiK15322.
    OMAiKLVCRRN.
    OrthoDBiEOG76X623.
    PhylomeDBiQ5M954.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR016589. tRNA_splic_SEN2.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5M954-1 [UniParc]FASTAAdd to Basket

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    MAEAVFRAPK RKRRVYESYE SPLPIPFSQD QSPRKEFRIF QAEMISNNVV    50
    VRGTEDMEQL YGKGYFGKGI LSRSRPNFTI SNPKLAARWK GVQTDMPIIT 100
    SEKYQHRVEW ARDFMRRQGH DESTVQKILT DYTEPLEPPY RERKGESPQH 150
    EPLSSKADSS LEGREGKDEL SVTTGGAGQS DDLQGLNTHS DCRQEGPGHA 200
    TLTVASPSSL NGHAIEDPEA LSQIPCCSQE ALGQQDDLWP EASSQIAGES 250
    RAAHEYVLIE EELCDVQEGA APHDELLKRK RLVCRRNPYR IFEYLQLSLE 300
    EAFFLAYALG CLSIYYEKEP LTIVKLWQAF TAVQPTFRTT YMAYHYFRSK 350
    GWVPKVGLKY GTDLLLYRKG PPFYHASYSV IIELVDDNFE GSLRRPFSWK 400
    SLAALSRVSG NVSKELMLCY LIKPSTMTNE DMETPECMRR IQVQEVILSR 450
    WVSSRERSDQ DEL 463
    Length:463
    Mass (Da):52,952
    Last modified:February 1, 2005 - v1
    Checksum:iF66CD72240AB7229
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC087631 mRNA. Translation: AAH87631.1.
    RefSeqiNP_001014079.1. NM_001014057.1.
    UniGeneiRn.206216.

    Genome annotation databases

    EnsembliENSRNOT00000056429; ENSRNOP00000053267; ENSRNOG00000027158.
    GeneIDi312649.
    KEGGirno:312649.
    UCSCiRGD:1309946. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC087631 mRNA. Translation: AAH87631.1 .
    RefSeqi NP_001014079.1. NM_001014057.1.
    UniGenei Rn.206216.

    3D structure databases

    ProteinModelPortali Q5M954.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000053267.

    PTM databases

    PhosphoSitei Q5M954.

    Proteomic databases

    PRIDEi Q5M954.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000056429 ; ENSRNOP00000053267 ; ENSRNOG00000027158 .
    GeneIDi 312649.
    KEGGi rno:312649.
    UCSCi RGD:1309946. rat.

    Organism-specific databases

    CTDi 80746.
    RGDi 1309946. Tsen2.

    Phylogenomic databases

    eggNOGi COG1676.
    GeneTreei ENSGT00390000013266.
    HOGENOMi HOG000154285.
    HOVERGENi HBG056610.
    KOi K15322.
    OMAi KLVCRRN.
    OrthoDBi EOG76X623.
    PhylomeDBi Q5M954.

    Miscellaneous databases

    NextBioi 665062.
    PROi Q5M954.

    Gene expression databases

    Genevestigatori Q5M954.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR016589. tRNA_splic_SEN2.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF011789. tRNA_splic_SEN2. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiSEN2_RAT
    AccessioniPrimary (citable) accession number: Q5M954
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3