Reviewed, UniProtKB/Swiss-Prot Q5M8Z0 (BHMT1_XENTR)

Names and origin

Protein names

Recommended name:
    Betaine--homocysteine S-methyltransferase 1
    EC=2.1.1.5

Gene names

Name:

bhmt

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline By similarity.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Betaine--homocysteine S-methyltransferase 1		PRO_0000273223
Regions
	Domain	8 – 311	304	Hcy-binding
Sites
	Metal binding	214	1	Zinc By similarity
	Metal binding	296	1	Zinc By similarity
	Metal binding	297	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5M8Z0-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 079A8C12A4A73CDB	FASTA	403	44,154
	10 20 30 40 50 60 MAPTGAKKGL LERLDAGEVV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF 70 80 90 100 110 120 LRAGANVMQT FTFYASDDKL ENRGNYVAKK ISGQKVNEAA CDIAREVANE GDALVAGGVS 130 140 150 160 170 180 QTPSYLSCKS EVEVKGIFRK QLDVFIKKNV DFLIAEYFEH VEEAVWAVEV LKESGKPVAA 190 200 210 220 230 240 TLCIGPQGDL NGVTPGECAV RLAKAGASVV GVNCHFDPMT CIATVKLMKE GLVAAKVKAH 250 260 270 280 290 300 LMTQPLAYHT PDCGKQGFID LPEFPFALEP RIVTRWDIHK YAREAYNLGV RYIGGCCGFE 310 320 330 340 350 360 PYHTRAIAEE LAPERGFLPP GSEKHGSWGS GLEMHTKPWV RARARRDYWE KLPPASGRPC 370 380 390 400 CPSMSKPDAW GVTKGDADLM QQKEATTEQQ LIDLFAKQCI KSN

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC087774 mRNA. Translation: AAH87774.1. BC089235 mRNA. Translation: AAH89235.1.
RefSeq	NP_001011217.1.
UniGene	Str.8632
3D structure databases
SMR	Q5M8Z0. Positions 7-395.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5M8Z0.
Genome annotation databases
Ensembl	ENSXETT00000001133; ENSXETP00000001133; ENSXETG00000000523; Xenopus tropicalis. [Genome view]
GeneID	496651.
KEGG	xtr:496651.
Organism-specific databases
CTD	496651.
Xenbase	XB-FEAT-1008381. bhmt.
Phylogenomic databases
HOVERGEN	Q5M8Z0.
Enzyme and pathway databases
BRENDA	2.1.1.5. 279072.
Gene expression databases
Bgee	Q5M8Z0.
Family and domain databases
InterPro	IPR017226. Betaine-hCys_S-MeTrfase_BHMT. IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF037505. Betaine_HMT. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHMT1_XENTR

Accession

Primary (citable) accession number: Q5M8Z0
Secondary accession number(s): Q5FWR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	February 1, 2005
Last modified:	September 1, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project