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Protein

N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)

Gene

Acy3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids (By similarity).By similarity

Catalytic activityi

An N-acyl-aromatic-L-amino acid + H2O = an aromatic-L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi21ZincBy similarity1
Metal bindingi24ZincBy similarity1
Binding sitei63SubstrateBy similarity1
Metal bindingi116ZincBy similarity1
Binding sitei178SubstrateBy similarity1
Binding sitei288SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-5423646 Aflatoxin activation and detoxification

Names & Taxonomyi

Protein namesi
Recommended name:
N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming) (EC:3.5.1.114)
Alternative name(s):
Aminoacylase-3
Short name:
ACY-3
Aspartoacylase-2
Gene namesi
Name:Acy3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1305394 Acy3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003633621 – 319N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)Add BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei318PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5M876
PRIDEiQ5M876

PTM databases

iPTMnetiQ5M876
PhosphoSitePlusiQ5M876

Expressioni

Gene expression databases

BgeeiENSRNOG00000017901
GenevisibleiQ5M876 RN

Interactioni

Subunit structurei

Exists as a mixture of homodimers and homotetramer, both catalytically active.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024112

Structurei

3D structure databases

ProteinModelPortaliQ5M876
SMRiQ5M876
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 210Hydrolytic domainBy similarityAdd BLAST210
Regioni70 – 71Substrate bindingBy similarity2
Regioni211 – 318Shielding domainBy similarityAdd BLAST108

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IERR Eukaryota
COG2988 LUCA
GeneTreeiENSGT00390000001189
HOGENOMiHOG000232489
HOVERGENiHBG004172
InParanoidiQ5M876
KOiK18458
OMAiDDPYEVT
OrthoDBiEOG091G0BL6
PhylomeDBiQ5M876
TreeFamiTF328708

Family and domain databases

CDDicd06909 M14_ASPA, 1 hit
HAMAPiMF_00704 Aspartoacylase, 1 hit
InterProiView protein in InterPro
IPR016708 Aspartoacylase
IPR007036 Aste_AspA
PfamiView protein in Pfam
PF04952 AstE_AspA, 1 hit
PIRSFiPIRSF018001 Aspartoacylase, 1 hit

Sequencei

Sequence statusi: Complete.

Q5M876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSLPGSRKP LLRVAVTGGT HGNEMCGVYL ARYWLQNPGE LQRPSFSAMP
60 70 80 90 100
VLANPAATAA CRRYIDRDLN RTFTLTFLGS TATPDDPYEV KRAQELNQLL
110 120 130 140 150
GPKGTCQAFD FILDLHNTTA NTGACLISEV SQNPFNLHLC HYLQLQNPGL
160 170 180 190 200
PCRLFQFEPP GTESYSMDSV SKNGISLELG PQPQGVLRAE LFSQMRAMVA
210 220 230 240 250
SILDFIELFN QGMEFPAFEM EVYKNLGSVD FPRTTDGHLT GTVHSRLQDH
260 270 280 290 300
DFEPLRPGEP IFKLFSGEDV LYEGDSVVYP LFVNEAAYYE KRVAFLKSEK
310
IRISVPALPG LTPSSTQTP
Length:319
Mass (Da):35,419
Last modified:February 1, 2005 - v1
Checksum:iAA6849FBE91D62E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088190 mRNA Translation: AAH88190.1
RefSeqiNP_001009603.1, NM_001009603.1
XP_006230795.1, XM_006230733.3
XP_008758320.1, XM_008760098.2
UniGeneiRn.22552

Genome annotation databases

EnsembliENSRNOT00000024112; ENSRNOP00000024112; ENSRNOG00000017901
GeneIDi293653
KEGGirno:293653
UCSCiRGD:1305394 rat

Similar proteinsi

Entry informationi

Entry nameiACY3_RAT
AccessioniPrimary (citable) accession number: Q5M876
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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