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Protein

N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)

Gene

Acy3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids (By similarity).By similarity

Catalytic activityi

An N-acyl-aromatic-L-amino acid + H2O = an aromatic-L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi21 – 211ZincBy similarity
Metal bindingi24 – 241ZincBy similarity
Binding sitei63 – 631SubstrateBy similarity
Metal bindingi116 – 1161ZincBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Binding sitei288 – 2881SubstrateBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-5423646. Aflatoxin activation and detoxification.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming) (EC:3.5.1.114)
Alternative name(s):
Aminoacylase-3
Short name:
ACY-3
Aspartoacylase-2
Gene namesi
Name:Acy3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1305394. Acy3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)PRO_0000363362Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei318 – 3181PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5M876.
PRIDEiQ5M876.

PTM databases

iPTMnetiQ5M876.
PhosphoSiteiQ5M876.

Expressioni

Gene expression databases

GenevisibleiQ5M876. RN.

Interactioni

Subunit structurei

Exists as a mixture of homodimers and homotetramer, both catalytically active.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024112.

Structurei

3D structure databases

ProteinModelPortaliQ5M876.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 210210Hydrolytic domainBy similarityAdd
BLAST
Regioni70 – 712Substrate bindingBy similarity
Regioni211 – 318108Shielding domainBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IERR. Eukaryota.
COG2988. LUCA.
GeneTreeiENSGT00390000001189.
HOGENOMiHOG000232489.
HOVERGENiHBG004172.
InParanoidiQ5M876.
KOiK18458.
OMAiTANMGTC.
OrthoDBiEOG78SQJ6.
PhylomeDBiQ5M876.
TreeFamiTF328708.

Family and domain databases

HAMAPiMF_00704. Aspartoacylase.
InterProiIPR016708. Aspartoacylase.
IPR007036. Aste_AspA.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF018001. Aspartoacylase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5M876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSLPGSRKP LLRVAVTGGT HGNEMCGVYL ARYWLQNPGE LQRPSFSAMP
60 70 80 90 100
VLANPAATAA CRRYIDRDLN RTFTLTFLGS TATPDDPYEV KRAQELNQLL
110 120 130 140 150
GPKGTCQAFD FILDLHNTTA NTGACLISEV SQNPFNLHLC HYLQLQNPGL
160 170 180 190 200
PCRLFQFEPP GTESYSMDSV SKNGISLELG PQPQGVLRAE LFSQMRAMVA
210 220 230 240 250
SILDFIELFN QGMEFPAFEM EVYKNLGSVD FPRTTDGHLT GTVHSRLQDH
260 270 280 290 300
DFEPLRPGEP IFKLFSGEDV LYEGDSVVYP LFVNEAAYYE KRVAFLKSEK
310
IRISVPALPG LTPSSTQTP
Length:319
Mass (Da):35,419
Last modified:February 1, 2005 - v1
Checksum:iAA6849FBE91D62E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088190 mRNA. Translation: AAH88190.1.
RefSeqiNP_001009603.1. NM_001009603.1.
XP_006230795.1. XM_006230733.2.
XP_008758320.1. XM_008760098.1.
UniGeneiRn.22552.

Genome annotation databases

EnsembliENSRNOT00000024112; ENSRNOP00000024112; ENSRNOG00000017901.
GeneIDi293653.
KEGGirno:293653.
UCSCiRGD:1305394. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088190 mRNA. Translation: AAH88190.1.
RefSeqiNP_001009603.1. NM_001009603.1.
XP_006230795.1. XM_006230733.2.
XP_008758320.1. XM_008760098.1.
UniGeneiRn.22552.

3D structure databases

ProteinModelPortaliQ5M876.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024112.

PTM databases

iPTMnetiQ5M876.
PhosphoSiteiQ5M876.

Proteomic databases

PaxDbiQ5M876.
PRIDEiQ5M876.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024112; ENSRNOP00000024112; ENSRNOG00000017901.
GeneIDi293653.
KEGGirno:293653.
UCSCiRGD:1305394. rat.

Organism-specific databases

CTDi91703.
RGDi1305394. Acy3.

Phylogenomic databases

eggNOGiENOG410IERR. Eukaryota.
COG2988. LUCA.
GeneTreeiENSGT00390000001189.
HOGENOMiHOG000232489.
HOVERGENiHBG004172.
InParanoidiQ5M876.
KOiK18458.
OMAiTANMGTC.
OrthoDBiEOG78SQJ6.
PhylomeDBiQ5M876.
TreeFamiTF328708.

Enzyme and pathway databases

ReactomeiR-RNO-5423646. Aflatoxin activation and detoxification.

Miscellaneous databases

PROiQ5M876.

Gene expression databases

GenevisibleiQ5M876. RN.

Family and domain databases

HAMAPiMF_00704. Aspartoacylase.
InterProiIPR016708. Aspartoacylase.
IPR007036. Aste_AspA.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF018001. Aspartoacylase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-318, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiACY3_RAT
AccessioniPrimary (citable) accession number: Q5M876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 1, 2005
Last modified: June 8, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.