Q5M872 (DPEP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dipeptidase 2
EC=3.4.13.19

Gene names

Name:

Dpep2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable metalloprotease which hydrolyzes leukotriene D4 (LTD4) into leukotriene E4 (LTE4) By similarity.
Catalytic activity	Hydrolysis of dipeptides.
Cofactor	Zinc By similarity.
Enzyme regulation	Inhibited by L-penicillamine By similarity.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the peptidase M19 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Dipeptidase Hydrolase Metalloprotease Protease
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	dipeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW dipeptidyl-peptidase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 462	432	Dipeptidase 2		PRO_0000231607
	Propeptide	463 – 481	19	Removed in mature form Potential		PRO_0000231608
Sites
	Metal binding	90	1	Zinc 1; catalytic By similarity
	Metal binding	92	1	Zinc 1; catalytic By similarity
	Metal binding	189	1	Zinc 1; catalytic By similarity
	Metal binding	189	1	Zinc 2; catalytic By similarity
	Metal binding	262	1	Zinc 2; catalytic By similarity
	Metal binding	283	1	Zinc 2; catalytic By similarity
	Binding site	216	1	Substrate By similarity
	Binding site	294	1	Substrate By similarity
	Binding site	352	1	Substrate By similarity
Amino acid modifications
	Lipidation	462	1	GPI-anchor amidated serine Potential
	Glycosylation	112	1	N-linked (GlcNAc...) By similarity
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Disulfide bond	139 ↔ 218		By similarity
	Disulfide bond	290 ↔ 322		By similarity
	Disulfide bond	427		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5M872 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: DC39B50C10A96AC1	FASTA	481	53,285
	10 20 30 40 50 60 MSLKGLEGHW VLSQVFLLVV VLLLLGPSEP LIRAQTKPGI ADASTAPSPL RTLTKPAIFS 70 80 90 100 110 120 IPTTPGNPNF PDLRDRTRAL MQDFPLIDGH NDLPLVLRQF YQNGLQDTNL RNFTHGQTSL 130 140 150 160 170 180 NRLKDGFVGA QFWSAYVPCQ TQDRDALRLT LEQIDLIRRM CASYSELELV TSVQALNSTQ 190 200 210 220 230 240 KLACLIGVEG GHSLDNSLAV LRSFYLLGVR YLTLTHTCNT PWAESSSKDV HSFYSSVKGL 250 260 270 280 290 300 TSFGEKVVAE MNRLGMMIDL SHVSDATARQ ALEVSQAPVI FSHSAARAVC PNARNLPDDI 310 320 330 340 350 360 LQLLKKNGGI VMVTFAVGVL PCNPLANVST VADHFDHIRT VIGSEFIGVG GDYDGTKQFP 370 380 390 400 410 420 QGLEDVSTYP VLIEELLRRG WGEQELQGVL RGNLLRVFRQ VEQVREKNKW QSPLEDMIPE 430 440 450 460 470 480 EQLDSACHSV LPHRRQYPEK DPPETPDSHT HKLSPKMPYS KSSPLRASSL TIMATFLGLL I

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC088195 mRNA. Translation: AAH88195.1.
IPI	IPI00392114.
RefSeq	NP_001011928.1. NM_001011928.1.
UniGene	Rn.20731.
3D structure databases
ProteinModelPortal	Q5M872.
SMR	Q5M872. Positions 72-430.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000031955.
Protein family/group databases
MEROPS	M19.004.
Proteomic databases
PRIDE	Q5M872.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000034722; ENSRNOP00000031955; ENSRNOG00000023303.
GeneID	291984.
KEGG	rno:291984.
UCSC	RGD:1305746. rat.
Organism-specific databases
CTD	64174.
RGD	1305746. Dpep2.
Phylogenomic databases
eggNOG	COG2355.
GeneTree	ENSGT00390000017920.
HOGENOM	HOG000072016.
HOVERGEN	HBG002339.
InParanoid	Q5M872.
OrthoDB	EOG4X97J0.
Gene expression databases
Genevestigator	Q5M872.
GermOnline	ENSRNOG00000023303. Rattus norvegicus.
Family and domain databases
InterPro	IPR000180. Pept_M19_AS. IPR008257. Peptidase_M19. [Graphical view]
PANTHER	PTHR10443. PTHR10443. 1 hit.
Pfam	PF01244. Peptidase_M19. 1 hit. [Graphical view]
PROSITE	PS00869. RENAL_DIPEPTIDASE_1. 1 hit. PS51365. RENAL_DIPEPTIDASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	633560.

Entry information

Entry name

DPEP2_RAT

Accession

Primary (citable) accession number: Q5M872

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	February 1, 2005
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program