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Protein

Signaling threshold-regulating transmembrane adapter 1

Gene

Sit1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates T-cell antigen receptor (TCR)-mediated signaling. Involved in positive selection of T-cells.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Signaling threshold-regulating transmembrane adapter 1
Alternative name(s):
SHP2-interacting transmembrane adapter protein
Suppression-inducing transmembrane adapter 1
Gene namesi
Name:Sit1
Synonyms:Sit
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1559919. Sit1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23ExtracellularSequence analysisAdd BLAST23
Transmembranei24 – 44Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini45 – 178CytoplasmicSequence analysisAdd BLAST134

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833421 – 178Signaling threshold-regulating transmembrane adapter 1Add BLAST178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi6N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi7InterchainBy similarity
Modified residuei62PhosphoserineBy similarity1
Modified residuei65PhosphoserineCombined sources1
Modified residuei72PhosphotyrosineBy similarity1
Modified residuei84PhosphoserineBy similarity1
Modified residuei87PhosphoserineCombined sources1
Modified residuei89PhosphoserineCombined sources1
Modified residuei126PhosphothreonineBy similarity1
Modified residuei130PhosphotyrosineBy similarity1
Modified residuei164PhosphoserineCombined sources1
Modified residuei170PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosines upon TCR activation; which promotes recruitment of PTPN11, GRB2 and CSK.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5M869.
PRIDEiQ5M869.

PTM databases

iPTMnetiQ5M869.
PhosphoSitePlusiQ5M869.

Expressioni

Tissue specificityi

Lymph node, spleen and thymus.1 Publication

Gene expression databases

BgeeiENSRNOG00000021546.
GenevisibleiQ5M869. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. When phosphorylated, interacts with PTPN11/SHP2, GRB2 and CSK (By similarity).By similarity

GO - Molecular functioni

Structurei

3D structure databases

SMRiQ5M869.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 75Interaction with GRB2By similarity4
Regioni128 – 133Interaction with PTPN11By similarity6
Regioni151 – 154Interaction with CSKBy similarity4
Regioni170 – 173Interaction with GRB2By similarity4

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYYR. Eukaryota.
ENOG4111C3Q. LUCA.
GeneTreeiENSGT00390000016476.
HOGENOMiHOG000231524.
HOVERGENiHBG080311.
InParanoidiQ5M869.
OMAiQAWGLWA.
OrthoDBiEOG091G0U43.
PhylomeDBiQ5M869.
TreeFamiTF337816.

Family and domain databases

InterProiView protein in InterPro
IPR033269. Sit1.
PANTHERiPTHR15604. PTHR15604. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD358400. PD358400. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5M869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRENNCTTA DLAWGIPSIT QAWGLWALFG VVTMLLLISL AALLSQWTRG
60 70 80 90 100
RRRTQEEQGP PSGRSVEEVP LYGNLHYLQT GRLSEESRSE EQDPSSGGLA
110 120 130 140 150
RGAEEATCYT SLQLRPAQGR IPSSGTPIKY CEVVLDSEPK PQASGPEPEL
160 170
YASVCAQTRR ARASFPDQAY ANSQPAPS
Length:178
Mass (Da):19,368
Last modified:February 1, 2005 - v1
Checksum:i518FD149CC3D7E2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088199 mRNA. Translation: AAH88199.1.
RefSeqiNP_001019515.1. NM_001024344.1.
UniGeneiRn.47894.

Genome annotation databases

EnsembliENSRNOT00000035040; ENSRNOP00000036171; ENSRNOG00000021546.
GeneIDi500449.
KEGGirno:500449.
UCSCiRGD:1559919. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088199 mRNA. Translation: AAH88199.1.
RefSeqiNP_001019515.1. NM_001024344.1.
UniGeneiRn.47894.

3D structure databases

SMRiQ5M869.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ5M869.
PhosphoSitePlusiQ5M869.

Proteomic databases

PaxDbiQ5M869.
PRIDEiQ5M869.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000035040; ENSRNOP00000036171; ENSRNOG00000021546.
GeneIDi500449.
KEGGirno:500449.
UCSCiRGD:1559919. rat.

Organism-specific databases

CTDi27240.
RGDi1559919. Sit1.

Phylogenomic databases

eggNOGiENOG410IYYR. Eukaryota.
ENOG4111C3Q. LUCA.
GeneTreeiENSGT00390000016476.
HOGENOMiHOG000231524.
HOVERGENiHBG080311.
InParanoidiQ5M869.
OMAiQAWGLWA.
OrthoDBiEOG091G0U43.
PhylomeDBiQ5M869.
TreeFamiTF337816.

Miscellaneous databases

PROiPR:Q5M869.

Gene expression databases

BgeeiENSRNOG00000021546.
GenevisibleiQ5M869. RN.

Family and domain databases

InterProiView protein in InterPro
IPR033269. Sit1.
PANTHERiPTHR15604. PTHR15604. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD358400. PD358400. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIT1_RAT
AccessioniPrimary (citable) accession number: Q5M869
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: May 10, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.