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Q5M868

- GBA2_RAT

UniProt

Q5M868 - GBA2_RAT

Protein

Non-lysosomal glucosylceramidase

Gene

Gba2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 2 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide (GlcCer) to free glucose and ceramide. Involved in sphingomyelin generation and prevention of glycolipid accumulation. May also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo By similarity. Plays a role in central nevous system development By similarity. Required for proper formation of motor neuron axons By similarity.By similarity

    Catalytic activityi

    D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

    Enzyme regulationi

    Enzymatic activity is dependent on membrane association and requires the presence of lipids.By similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB
    2. glucosylceramidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. bile acid metabolic process Source: UniProtKB
    2. central nervous system neuron development Source: UniProtKB
    3. glucosylceramide catabolic process Source: InterPro
    4. glycoside catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_198596. Glycosphingolipid metabolism.

    Protein family/group databases

    CAZyiGH116. Glycoside Hydrolase Family 116.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-lysosomal glucosylceramidase (EC:3.2.1.45)
    Short name:
    NLGase
    Alternative name(s):
    Beta-glucocerebrosidase 2
    Short name:
    Beta-glucosidase 2
    Glucosylceramidase 2
    Gene namesi
    Name:Gba2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1305598. Gba2.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Not localized to lipid rafts.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 912912Non-lysosomal glucosylceramidasePRO_0000283760Add
    BLAST

    Proteomic databases

    PaxDbiQ5M868.
    PRIDEiQ5M868.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5M868.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000022002.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5M868.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG4354.
    HOGENOMiHOG000234168.
    HOVERGENiHBG105975.
    InParanoidiQ5M868.
    PhylomeDBiQ5M868.
    TreeFamiTF313888.

    Family and domain databases

    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR014551. Beta_glucosidase_GBA2-type.
    IPR024462. GBA2_N.
    IPR006775. Glucosylceramidase.
    [Graphical view]
    PfamiPF04685. DUF608. 1 hit.
    PF12215. GBA2_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF028944. Beta_gluc_GBA2. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5M868-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVTCVPASEQ IGCAERDSQI YSEDTGGTEA VRVTDCRSPE DSGPQNEPGY    50
    CNSEDSGQLM ASYEGKARGY QVPPFGWRIC LAHEFAEKRK PFQANNVSLS 100
    NLVKHFGMGL RYLKWWYRKT QVEKKTPFID MFNSVPLRQI YGCPLGGIGG 150
    GTITRGWRGQ FCRWQLNPGM YQHQTVIADQ FIVCLRRDGR TVYQQVLSLE 200
    LPSVLRSWNW GLCGYFAFYH ALYPRAWTVY QLPGQNVTLT CRQITPILPH 250
    DYQDSSLPVG VFVWDVENEG DETLDVSIMF SMRNGLGGED DAAGGLWNEP 300
    FRLEQDGTTV QGLLLHHPTP PNPYTMAVAA RHTADTTVTY TTAFDPDSTG 350
    QQVWQDLLQD GQLDSPAGQS TPTQRGEGVA GAVCASSKLL PRGRCCLEFS 400
    LAWDMPRIMF GAKGQVHYRR YTRFFGSDGD VAPALSHYAL CQYAGWENSI 450
    SAWQNPVLDD RSLPAWYKSA LFNELYFLAD GGTVWLEVPE DSLPEELGGS 500
    MYQLRPILQD YGRFGYLEGQ EYRMYNTYDV HFYASFALVM LWPKLELSLQ 550
    YDMALATFKE DLTRRRYLMS GVVAPVKRRN VIPHDIGDPD DEPWLRVNAY 600
    LIHDTADWKD LNLKFVLQVY RDYYLTGDQG FLKDMWPVCL AVMESEMKFD 650
    KDQDGLIENG GYADQTYDGW VTTGPSAYCG GLWLAAVAVM VQMAVLCGAQ 700
    DVQDKFSSIL CRGREAYERL LWNGRYYNYD SSSQPQSRSV MSDQCAGQWF 750
    LRACGLGEGD TEVFPTLHVV RALKTIFELN VQAFAGGAMG AVNGMQPHGV 800
    PDRSSVQSDE VWVGVVYGLA ATMIQEGLTW EGFRTAEGCY RTVWERLGLA 850
    FQTPEAYCQQ RVFRSLAYMR PLSIWAMQLA LQQQQHKKNS SRPAVTQGTA 900
    PSQPECGPKR SL 912
    Length:912
    Mass (Da):102,747
    Last modified:April 3, 2007 - v2
    Checksum:iC4A47C8C5F3D248A
    GO
    Isoform 2 (identifier: Q5M868-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         181-195: FIVCLRRDGRTVYQQ → VRKGAGRRRSDSWLA
         196-912: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:195
    Mass (Da):21,993
    Checksum:i15E7B9B8FA13E3A3
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei181 – 19515FIVCL…TVYQQ → VRKGAGRRRSDSWLA in isoform 2. 1 PublicationVSP_024385Add
    BLAST
    Alternative sequencei196 – 912717Missing in isoform 2. 1 PublicationVSP_024386Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AABR03040369 Genomic DNA. No translation available.
    BC088200 mRNA. Translation: AAH88200.1.
    UniGeneiRn.146071.

    Genome annotation databases

    UCSCiRGD:1305598. rat. [Q5M868-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AABR03040369 Genomic DNA. No translation available.
    BC088200 mRNA. Translation: AAH88200.1 .
    UniGenei Rn.146071.

    3D structure databases

    ProteinModelPortali Q5M868.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000022002.

    Protein family/group databases

    CAZyi GH116. Glycoside Hydrolase Family 116.

    Proteomic databases

    PaxDbi Q5M868.
    PRIDEi Q5M868.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:1305598. rat. [Q5M868-1 ]

    Organism-specific databases

    RGDi 1305598. Gba2.

    Phylogenomic databases

    eggNOGi COG4354.
    HOGENOMi HOG000234168.
    HOVERGENi HBG105975.
    InParanoidi Q5M868.
    PhylomeDBi Q5M868.
    TreeFami TF313888.

    Enzyme and pathway databases

    Reactomei REACT_198596. Glycosphingolipid metabolism.

    Miscellaneous databases

    PROi Q5M868.

    Gene expression databases

    Genevestigatori Q5M868.

    Family and domain databases

    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR014551. Beta_glucosidase_GBA2-type.
    IPR024462. GBA2_N.
    IPR006775. Glucosylceramidase.
    [Graphical view ]
    Pfami PF04685. DUF608. 1 hit.
    PF12215. GBA2_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF028944. Beta_gluc_GBA2. 1 hit.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
      , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
      Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Spleen.

    Entry informationi

    Entry nameiGBA2_RAT
    AccessioniPrimary (citable) accession number: Q5M868
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 68 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3