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Q5M868

- GBA2_RAT

UniProt

Q5M868 - GBA2_RAT

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Protein

Non-lysosomal glucosylceramidase

Gene

Gba2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide (GlcCer) to free glucose and ceramide. Involved in sphingomyelin generation and prevention of glycolipid accumulation. May also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo (By similarity). Plays a role in central nevous system development (By similarity). Required for proper formation of motor neuron axons (By similarity).By similarity

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Enzyme regulationi

Enzymatic activity is dependent on membrane association and requires the presence of lipids.By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB
  2. glucosylceramidase activity Source: UniProtKB-EC

GO - Biological processi

  1. bile acid metabolic process Source: UniProtKB
  2. central nervous system neuron development Source: UniProtKB
  3. glucosylceramide catabolic process Source: InterPro
  4. glycoside catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiREACT_198596. Glycosphingolipid metabolism.

Protein family/group databases

CAZyiGH116. Glycoside Hydrolase Family 116.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-lysosomal glucosylceramidase (EC:3.2.1.45)
Short name:
NLGase
Alternative name(s):
Beta-glucocerebrosidase 2
Short name:
Beta-glucosidase 2
Glucosylceramidase 2
Gene namesi
Name:Gba2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1305598. Gba2.

Subcellular locationi

Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Not localized to lipid rafts.By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912Non-lysosomal glucosylceramidasePRO_0000283760Add
BLAST

Proteomic databases

PaxDbiQ5M868.
PRIDEiQ5M868.

Expressioni

Gene expression databases

ExpressionAtlasiQ5M868. baseline and differential.
GenevestigatoriQ5M868.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022002.

Structurei

3D structure databases

ProteinModelPortaliQ5M868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4354.
HOGENOMiHOG000234168.
HOVERGENiHBG105975.
InParanoidiQ5M868.
PhylomeDBiQ5M868.
TreeFamiTF313888.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR014551. Beta_glucosidase_GBA2-type.
IPR024462. GBA2_N.
IPR006775. Glucosylceramidase.
[Graphical view]
PfamiPF04685. DUF608. 1 hit.
PF12215. GBA2_N. 1 hit.
[Graphical view]
PIRSFiPIRSF028944. Beta_gluc_GBA2. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q5M868-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVTCVPASEQ IGCAERDSQI YSEDTGGTEA VRVTDCRSPE DSGPQNEPGY
60 70 80 90 100
CNSEDSGQLM ASYEGKARGY QVPPFGWRIC LAHEFAEKRK PFQANNVSLS
110 120 130 140 150
NLVKHFGMGL RYLKWWYRKT QVEKKTPFID MFNSVPLRQI YGCPLGGIGG
160 170 180 190 200
GTITRGWRGQ FCRWQLNPGM YQHQTVIADQ FIVCLRRDGR TVYQQVLSLE
210 220 230 240 250
LPSVLRSWNW GLCGYFAFYH ALYPRAWTVY QLPGQNVTLT CRQITPILPH
260 270 280 290 300
DYQDSSLPVG VFVWDVENEG DETLDVSIMF SMRNGLGGED DAAGGLWNEP
310 320 330 340 350
FRLEQDGTTV QGLLLHHPTP PNPYTMAVAA RHTADTTVTY TTAFDPDSTG
360 370 380 390 400
QQVWQDLLQD GQLDSPAGQS TPTQRGEGVA GAVCASSKLL PRGRCCLEFS
410 420 430 440 450
LAWDMPRIMF GAKGQVHYRR YTRFFGSDGD VAPALSHYAL CQYAGWENSI
460 470 480 490 500
SAWQNPVLDD RSLPAWYKSA LFNELYFLAD GGTVWLEVPE DSLPEELGGS
510 520 530 540 550
MYQLRPILQD YGRFGYLEGQ EYRMYNTYDV HFYASFALVM LWPKLELSLQ
560 570 580 590 600
YDMALATFKE DLTRRRYLMS GVVAPVKRRN VIPHDIGDPD DEPWLRVNAY
610 620 630 640 650
LIHDTADWKD LNLKFVLQVY RDYYLTGDQG FLKDMWPVCL AVMESEMKFD
660 670 680 690 700
KDQDGLIENG GYADQTYDGW VTTGPSAYCG GLWLAAVAVM VQMAVLCGAQ
710 720 730 740 750
DVQDKFSSIL CRGREAYERL LWNGRYYNYD SSSQPQSRSV MSDQCAGQWF
760 770 780 790 800
LRACGLGEGD TEVFPTLHVV RALKTIFELN VQAFAGGAMG AVNGMQPHGV
810 820 830 840 850
PDRSSVQSDE VWVGVVYGLA ATMIQEGLTW EGFRTAEGCY RTVWERLGLA
860 870 880 890 900
FQTPEAYCQQ RVFRSLAYMR PLSIWAMQLA LQQQQHKKNS SRPAVTQGTA
910
PSQPECGPKR SL
Length:912
Mass (Da):102,747
Last modified:April 3, 2007 - v2
Checksum:iC4A47C8C5F3D248A
GO
Isoform 2 (identifier: Q5M868-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     181-195: FIVCLRRDGRTVYQQ → VRKGAGRRRSDSWLA
     196-912: Missing.

Note: No experimental confirmation available.

Show »
Length:195
Mass (Da):21,993
Checksum:i15E7B9B8FA13E3A3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei181 – 19515FIVCL…TVYQQ → VRKGAGRRRSDSWLA in isoform 2. 1 PublicationVSP_024385Add
BLAST
Alternative sequencei196 – 912717Missing in isoform 2. 1 PublicationVSP_024386Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03040369 Genomic DNA. No translation available.
BC088200 mRNA. Translation: AAH88200.1.
UniGeneiRn.146071.

Genome annotation databases

UCSCiRGD:1305598. rat. [Q5M868-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03040369 Genomic DNA. No translation available.
BC088200 mRNA. Translation: AAH88200.1 .
UniGenei Rn.146071.

3D structure databases

ProteinModelPortali Q5M868.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000022002.

Protein family/group databases

CAZyi GH116. Glycoside Hydrolase Family 116.

Proteomic databases

PaxDbi Q5M868.
PRIDEi Q5M868.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:1305598. rat. [Q5M868-1 ]

Organism-specific databases

RGDi 1305598. Gba2.

Phylogenomic databases

eggNOGi COG4354.
HOGENOMi HOG000234168.
HOVERGENi HBG105975.
InParanoidi Q5M868.
PhylomeDBi Q5M868.
TreeFami TF313888.

Enzyme and pathway databases

Reactomei REACT_198596. Glycosphingolipid metabolism.

Miscellaneous databases

PROi Q5M868.

Gene expression databases

ExpressionAtlasi Q5M868. baseline and differential.
Genevestigatori Q5M868.

Family and domain databases

InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR014551. Beta_glucosidase_GBA2-type.
IPR024462. GBA2_N.
IPR006775. Glucosylceramidase.
[Graphical view ]
Pfami PF04685. DUF608. 1 hit.
PF12215. GBA2_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF028944. Beta_gluc_GBA2. 1 hit.
SUPFAMi SSF48208. SSF48208. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Spleen.

Entry informationi

Entry nameiGBA2_RAT
AccessioniPrimary (citable) accession number: Q5M868
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: October 29, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3