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Protein

PAXIP1-associated glutamate-rich protein 1

Gene

PAGR1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Its association with the histone methyltransferase MLL2/MLL3 complex is suggesting a role in epigenetic transcriptional activation. However, in association with PAXIP1/PTIP is proposed to function at least in part independently of the MLL2/MLL3 complex. Proposed to be recruited by PAXIP1 to sites of DNA damage where the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage independently of the MLL2/MLL3 complex. However, its function in DNA damage has been questioned. During immunoglobulin class switching in activated B-cells is involved in transcription regulation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus independently of the MLL2/MLL3 complex. Involved in both estrogen receptor-regulated gene transcription and estrogen-stimulated G1/S cell-cycle transition. Acts as transcriptional cofactor for nuclear hormone receptors. Inhibits the induction properties of several steroid receptors such as NR3C1, AR and PPARG; the mechanism of inhibition appears to be gene-dependent.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
PAXIP1-associated glutamate-rich protein 1
Alternative name(s):
PAXIP1-associated protein 1
PTIP-associated protein 1
Gene namesi
Name:PAGR1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1305592. RGD1305592.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253PAXIP1-associated glutamate-rich protein 1PRO_0000248336Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei137 – 1371PhosphothreonineCombined sources
Modified residuei142 – 1421PhosphoserineCombined sources
Modified residuei147 – 1471PhosphoserineCombined sources
Modified residuei236 – 2361PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5M865.
PRIDEiQ5M865.

PTM databases

iPTMnetiQ5M865.

Expressioni

Gene expression databases

GenevisibleiQ5M865. RN.

Interactioni

Subunit structurei

Component of the KMT2 family MLL2/MLL3 complex, at least composed of the histone methyltransferases KMT2D and/or KMT2C, the common subunits ASH2L, RBBP5, WDR5 and DPY30, and the complex type-specific subunits PAXIP1/PTIP, PAGR1, NCOA6 and KDM6A; PAXIP1 is required for the association with the MLL2/MLL3 complex (By similarity). Forms a constitutive complex with PAXIP1/PTIP independently of the MLL2/MLL3 complex. Interacts with NCOA1, ESR1, NR3C1, AR.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027392.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni115 – 15945Sufficient for interaction with NCOA1By similarityAdd
BLAST
Regioni160 – 25394Sufficient for interaction with ESR1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi47 – 159113Glu-richAdd
BLAST

Phylogenomic databases

eggNOGiENOG410IXWI. Eukaryota.
ENOG4111Q6K. LUCA.
GeneTreeiENSGT00390000016049.
HOGENOMiHOG000115419.
HOVERGENiHBG080642.
InParanoidiQ5M865.
KOiK14973.
OMAiEDPKNWM.
OrthoDBiEOG7GQXXC.
PhylomeDBiQ5M865.
TreeFamiTF326621.

Family and domain databases

InterProiIPR028213. PA1.
[Graphical view]
PfamiPF15364. PAXIP1_C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5M865-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLALGHGTI AGSTAAPLSE EGEVTSGLQA LAVEDTGGPS VSASKAEEEG
60 70 80 90 100
KGSQEEAGRE ASRAEEALEA SSAVSDERAE GESEDWCVPC SDEEVELPAN
110 120 130 140 150
GQSWMPPPSE IQRLYELLAT QGTLELQAEI LPRRPPTPEA QSEEERSDEE
160 170 180 190 200
PEAKEEEEEK PHMPTEFDFD DEPVTPKDSL IDRRRTPGSS ARSQKREARL
210 220 230 240 250
DKVLSDMKRH KKLEEQILRT GRDLFSLDSE GPSPASPPLR SSGNSLFPRQ

RKY
Length:253
Mass (Da):27,683
Last modified:September 5, 2006 - v2
Checksum:iBF5C8549355D152C
GO
Isoform 2 (identifier: Q5M865-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     161-253: Missing.

Note: No experimental confirmation available.
Show »
Length:160
Mass (Da):17,009
Checksum:i33043A0F02BBC5B5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei161 – 25393Missing in isoform 2. 1 PublicationVSP_020244Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03004725 Genomic DNA. No translation available.
BC088203 mRNA. Translation: AAH88203.1.
RefSeqiNP_001013923.1. NM_001013901.1. [Q5M865-2]
UniGeneiRn.159917.

Genome annotation databases

EnsembliENSRNOT00000027392; ENSRNOP00000027392; ENSRNOG00000020217. [Q5M865-1]
GeneIDi293500.
KEGGirno:293500.
UCSCiRGD:1305592. rat. [Q5M865-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03004725 Genomic DNA. No translation available.
BC088203 mRNA. Translation: AAH88203.1.
RefSeqiNP_001013923.1. NM_001013901.1. [Q5M865-2]
UniGeneiRn.159917.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027392.

PTM databases

iPTMnetiQ5M865.

Proteomic databases

PaxDbiQ5M865.
PRIDEiQ5M865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027392; ENSRNOP00000027392; ENSRNOG00000020217. [Q5M865-1]
GeneIDi293500.
KEGGirno:293500.
UCSCiRGD:1305592. rat. [Q5M865-1]

Organism-specific databases

CTDi79447.
RGDi1305592. RGD1305592.

Phylogenomic databases

eggNOGiENOG410IXWI. Eukaryota.
ENOG4111Q6K. LUCA.
GeneTreeiENSGT00390000016049.
HOGENOMiHOG000115419.
HOVERGENiHBG080642.
InParanoidiQ5M865.
KOiK14973.
OMAiEDPKNWM.
OrthoDBiEOG7GQXXC.
PhylomeDBiQ5M865.
TreeFamiTF326621.

Miscellaneous databases

PROiQ5M865.

Gene expression databases

GenevisibleiQ5M865. RN.

Family and domain databases

InterProiIPR028213. PA1.
[Graphical view]
PfamiPF15364. PAXIP1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Thymus.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-137; SER-142; SER-147 AND SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPAGR1_RAT
AccessioniPrimary (citable) accession number: Q5M865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: June 8, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The terminology of MLL proteins in mammalia is not consistent also concerning the terminology of MLL protein-containing complexes. The decribed MLL2/MLL3 complex is commonly described as MLL3/MLL4 complex in literature.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.