Reviewed, UniProtKB/Swiss-Prot Q5M854 (MGT4A_RAT)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
    EC=2.4.1.145
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
      Short name=N-acetylglucosaminyltransferase IVa
      Short name=GlcNAc-T IVa
      Short name=GnT-IVa
Cleaved into the following chain:
    1- Recommended name:
            Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form

Gene names

Name:

Mgat4a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form: Secreted By similarity.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Domain	Coiled coil Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A		PRO_0000288589
	Chain	84 – 526	443	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form By similarity		PRO_0000288590
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 526	499	Lumenal Potential
	Coiled coil	28 – 63	36	Potential
Amino acid modifications
	Glycosylation	449	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5M854-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: DB998ED2CC79D7D9	FASTA	526	60,590
	10 20 30 40 50 60 MRLRNGTVAT ALVFVTSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRV AEHRISQRSS 70 80 90 100 110 120 ELNTIVQQFR RAGAETNGNN TIKLLKELTS KKSLQVPSIY YHLPHLLQNE RSLQPAVQIG 130 140 150 160 170 180 SGRTGVSIVM GIPTVKREVK SYLIETLHSL IDNLYPEEKL DCVIVVFIGE TDLDYVHSVV 190 200 210 220 230 240 ANLEKEFSRE ISSGLLEVIS PPESYYPDLT NLKETFGDSK ERVRWRTKQN LDYCFLMMYA 250 260 270 280 290 300 QEKGIYYIQL EDDIIVKQNY FNTIKNFALQ LSSEEWMILE FSQLGFIGKM FQAPDLALVV 310 320 330 340 350 360 EFILMFYKEK PIDWLLDHIL WVKVCNPEKD AKHCDRQKAN LRIRFRPSLF QHVGLHSSLS 370 380 390 400 410 420 GKIQKLTDKD YMKPLLLKIH VNPPAEVSTS LKVYQGHTLE KTYMGEDFFW AITPTAGDYI 430 440 450 460 470 480 LFKFDKPVNV ESYLFHSGNQ EHPGDILLNT TVEVLPLKSD SLEISKETKD KRLEDGYFRI 490 500 510 520 GKFEYGVAEG IVDPGLNPIS AFRLSVIQNS AVWAILNEIH IKKVTS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC088215 mRNA. Translation: AAH88215.1.
IPI	IPI00360706.
RefSeq	NP_001012225.1. NP_001153627.1.
UniGene	Rn.17906
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5M854.
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Genome annotation databases
Ensembl	ENSRNOT00000024701; ENSRNOP00000024701; ENSRNOG00000018276; Rattus norvegicus. [Genome view]
GeneID	367252.
KEGG	rno:367252.
UCSC	NM_001012225. rat.
Organism-specific databases
CTD	367252.
RGD	1305650. Mgat4a.
Phylogenomic databases
eggNOG	roNOG12162.
HOVERGEN	Q5M854.
InParanoid	Q5M854.
Enzyme and pathway databases
BRENDA	2.4.1.145. 248.
Gene expression databases
Genevestigator	Q5M854.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	690965.

Entry information

Entry name

MGT4A_RAT

Accession

Primary (citable) accession number: Q5M854

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)