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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

Mgat4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-(beta-D-GlcNAc-(1->4))-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein].

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandMetal-binding

Enzyme and pathway databases

UniPathwayiUPA00378

Protein family/group databases

CAZyiGT54 Glycosyltransferase Family 54

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name:
GlcNAc-T IVa
Short name:
GnT-IVa
Short name:
N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
Cleaved into the following chain:
Gene namesi
Name:Mgat4a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi1305650 Mgat4a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 526LumenalSequence analysisAdd BLAST499

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002885891 – 526Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase AAdd BLAST526
ChainiPRO_000028859084 – 526Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble formBy similarityAdd BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi449N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei465PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5M854

PTM databases

iPTMnetiQ5M854
PhosphoSitePlusiQ5M854

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024701

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili28 – 63Sequence analysisAdd BLAST36

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEDC Eukaryota
ENOG410XPV0 LUCA
HOGENOMiHOG000015085
HOVERGENiHBG053194
InParanoidiQ5M854
KOiK00738
PhylomeDBiQ5M854

Family and domain databases

InterProiView protein in InterPro
IPR006759 Glyco_transf_54
PANTHERiPTHR12062 PTHR12062, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5M854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNGTVAT ALVFVTSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRV
60 70 80 90 100
AEHRISQRSS ELNTIVQQFR RAGAETNGNN TIKLLKELTS KKSLQVPSIY
110 120 130 140 150
YHLPHLLQNE RSLQPAVQIG SGRTGVSIVM GIPTVKREVK SYLIETLHSL
160 170 180 190 200
IDNLYPEEKL DCVIVVFIGE TDLDYVHSVV ANLEKEFSRE ISSGLLEVIS
210 220 230 240 250
PPESYYPDLT NLKETFGDSK ERVRWRTKQN LDYCFLMMYA QEKGIYYIQL
260 270 280 290 300
EDDIIVKQNY FNTIKNFALQ LSSEEWMILE FSQLGFIGKM FQAPDLALVV
310 320 330 340 350
EFILMFYKEK PIDWLLDHIL WVKVCNPEKD AKHCDRQKAN LRIRFRPSLF
360 370 380 390 400
QHVGLHSSLS GKIQKLTDKD YMKPLLLKIH VNPPAEVSTS LKVYQGHTLE
410 420 430 440 450
KTYMGEDFFW AITPTAGDYI LFKFDKPVNV ESYLFHSGNQ EHPGDILLNT
460 470 480 490 500
TVEVLPLKSD SLEISKETKD KRLEDGYFRI GKFEYGVAEG IVDPGLNPIS
510 520
AFRLSVIQNS AVWAILNEIH IKKVTS
Length:526
Mass (Da):60,590
Last modified:February 1, 2005 - v1
Checksum:iDB998ED2CC79D7D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088215 mRNA Translation: AAH88215.1
RefSeqiNP_001012225.1, NM_001012225.2
NP_001153627.1, NM_001160155.1
UniGeneiRn.17906

Genome annotation databases

GeneIDi367252
KEGGirno:367252
UCSCiRGD:1305650 rat

Entry informationi

Entry nameiMGT4A_RAT
AccessioniPrimary (citable) accession number: Q5M854
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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