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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

Mgat4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name:
GlcNAc-T IVa
Short name:
GnT-IVa
Short name:
N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
Cleaved into the following chain:
Gene namesi
Name:Mgat4a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1305650. Mgat4a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 526499LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase APRO_0000288589Add
BLAST
Chaini84 – 526443Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble formBy similarityPRO_0000288590Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence analysis
Modified residuei465 – 4651PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5M854.

PTM databases

iPTMnetiQ5M854.
PhosphoSiteiQ5M854.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024701.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili28 – 6336Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiQ5M854.
KOiK00738.
PhylomeDBiQ5M854.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5M854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNGTVAT ALVFVTSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRV
60 70 80 90 100
AEHRISQRSS ELNTIVQQFR RAGAETNGNN TIKLLKELTS KKSLQVPSIY
110 120 130 140 150
YHLPHLLQNE RSLQPAVQIG SGRTGVSIVM GIPTVKREVK SYLIETLHSL
160 170 180 190 200
IDNLYPEEKL DCVIVVFIGE TDLDYVHSVV ANLEKEFSRE ISSGLLEVIS
210 220 230 240 250
PPESYYPDLT NLKETFGDSK ERVRWRTKQN LDYCFLMMYA QEKGIYYIQL
260 270 280 290 300
EDDIIVKQNY FNTIKNFALQ LSSEEWMILE FSQLGFIGKM FQAPDLALVV
310 320 330 340 350
EFILMFYKEK PIDWLLDHIL WVKVCNPEKD AKHCDRQKAN LRIRFRPSLF
360 370 380 390 400
QHVGLHSSLS GKIQKLTDKD YMKPLLLKIH VNPPAEVSTS LKVYQGHTLE
410 420 430 440 450
KTYMGEDFFW AITPTAGDYI LFKFDKPVNV ESYLFHSGNQ EHPGDILLNT
460 470 480 490 500
TVEVLPLKSD SLEISKETKD KRLEDGYFRI GKFEYGVAEG IVDPGLNPIS
510 520
AFRLSVIQNS AVWAILNEIH IKKVTS
Length:526
Mass (Da):60,590
Last modified:February 1, 2005 - v1
Checksum:iDB998ED2CC79D7D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088215 mRNA. Translation: AAH88215.1.
RefSeqiNP_001012225.1. NM_001012225.2.
NP_001153627.1. NM_001160155.1.
UniGeneiRn.17906.

Genome annotation databases

GeneIDi367252.
KEGGirno:367252.
UCSCiRGD:1305650. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088215 mRNA. Translation: AAH88215.1.
RefSeqiNP_001012225.1. NM_001012225.2.
NP_001153627.1. NM_001160155.1.
UniGeneiRn.17906.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024701.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

PTM databases

iPTMnetiQ5M854.
PhosphoSiteiQ5M854.

Proteomic databases

PaxDbiQ5M854.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi367252.
KEGGirno:367252.
UCSCiRGD:1305650. rat.

Organism-specific databases

CTDi11320.
RGDi1305650. Mgat4a.

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiQ5M854.
KOiK00738.
PhylomeDBiQ5M854.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ5M854.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGT4A_RAT
AccessioniPrimary (citable) accession number: Q5M854
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.