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Protein

Calcium release-activated calcium channel protein 1

Gene

Orai1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.By similarity

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • positive regulation of calcium ion transport Source: UniProtKB
  • store-operated calcium entry Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Adaptive immunity, Calcium transport, Immunity, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium release-activated calcium channel protein 1
Alternative name(s):
Protein orai-1
Transmembrane protein 142A
Gene namesi
Name:Orai1
Synonyms:Cracm1, Tmem142a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1311873. Orai1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cytoplasmic vesicleautophagosome By similarity

  • Note: Colocalizes with UBQLN1 in the autophagosome.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8888CytoplasmicBy similarityAdd
BLAST
Transmembranei89 – 10618HelicalSequence analysisAdd
BLAST
Topological domaini107 – 12014ExtracellularSequence analysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence analysisAdd
BLAST
Topological domaini142 – 17433CytoplasmicSequence analysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence analysisAdd
BLAST
Topological domaini196 – 23742ExtracellularSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Topological domaini259 – 30446CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • autophagosome Source: UniProtKB-SubCell
  • cytoplasmic vesicle Source: UniProtKB-KW
  • growth cone Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Calcium release-activated calcium channel protein 1PRO_0000234383Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence analysis
Modified residuei298 – 2981PhosphothreonineBy similarity

Post-translational modificationi

N-glycosylated.By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5M848.

Interactioni

Subunit structurei

Interacts with STIM1 and STIM2; this regulates channel activity (By similarity). Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity (PubMed:23109337). Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+ (By similarity). Forms a complex with CRACR2A/EFCAB4B and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations (By similarity). Interacts with SLC35G1 (By similarity). Interacts with UBQLN1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Stim2D4A5X13EBI-9700451,EBI-9700435

Protein-protein interaction databases

IntActiQ5M848. 2 interactions.
STRINGi10116.ENSRNOP00000001806.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 9121Interaction with STIM1By similarityAdd
BLAST
Regioni275 – 29521Interaction with STIM1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 497Poly-Pro

Sequence similaritiesi

Belongs to the Orai family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4298. Eukaryota.
ENOG4110DSB. LUCA.
HOGENOMiHOG000246925.
HOVERGENiHBG081343.
InParanoidiQ5M848.
KOiK16056.
PhylomeDBiQ5M848.

Family and domain databases

InterProiIPR012446. CRAC_channel.
[Graphical view]
PANTHERiPTHR31501. PTHR31501. 2 hits.
PfamiPF07856. Orai-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5M848-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPEPAPPPN NSNPELPLSG GSSTSGSRRS RRRSGDGEPT GAPPLPPPPA
60 70 80 90 100
VSYPDWIGQS YSEVMSLNEH SMQALSWRKL YLSRAKLKAS SRTSALLSGF
110 120 130 140 150
AMVAMVEVQL DTDHDYPPGL LIVFSACTTV LVAVHLFALM ISTCILPNIE
160 170 180 190 200
AVSNVHNLNS VKESPHERMH RHIELAWAFS TVIGTLLFLA EVVLLCWVKF
210 220 230 240 250
LPLKRQAGQP SPTKPPTEPA VVVANSSNNG GITPGEAAAI ASTAIMVPCG
260 270 280 290 300
LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG

THYA
Length:304
Mass (Da):33,040
Last modified:February 1, 2005 - v1
Checksum:i98264D8880982C74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088225 mRNA. Translation: AAH88225.1.
RefSeqiNP_001014004.1. NM_001013982.1.
UniGeneiRn.3869.

Genome annotation databases

GeneIDi304496.
KEGGirno:304496.
UCSCiRGD:1311873. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088225 mRNA. Translation: AAH88225.1.
RefSeqiNP_001014004.1. NM_001013982.1.
UniGeneiRn.3869.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5M848. 2 interactions.
STRINGi10116.ENSRNOP00000001806.

Proteomic databases

PaxDbiQ5M848.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi304496.
KEGGirno:304496.
UCSCiRGD:1311873. rat.

Organism-specific databases

CTDi84876.
RGDi1311873. Orai1.

Phylogenomic databases

eggNOGiKOG4298. Eukaryota.
ENOG4110DSB. LUCA.
HOGENOMiHOG000246925.
HOVERGENiHBG081343.
InParanoidiQ5M848.
KOiK16056.
PhylomeDBiQ5M848.

Miscellaneous databases

NextBioi653128.
PROiQ5M848.

Family and domain databases

InterProiIPR012446. CRAC_channel.
[Graphical view]
PANTHERiPTHR31501. PTHR31501. 2 hits.
PfamiPF07856. Orai-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  2. "Crystal structure of calmodulin binding domain of orai1 in complex with Ca2+ calmodulin displays a unique binding mode."
    Liu Y., Zheng X., Mueller G.A., Sobhany M., DeRose E.F., Zhang Y., London R.E., Birnbaumer L.
    J. Biol. Chem. 287:43030-43041(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CALM.

Entry informationi

Entry nameiCRCM1_RAT
AccessioniPrimary (citable) accession number: Q5M848
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: February 1, 2005
Last modified: November 11, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.