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Protein

Nesprin-4

Gene

Syne4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Behaves as a kinesin cargo, providing a functional binding site for kinesin-1 at the nuclear envelope. Hence may contribute to the establishment of secretory epithelial morphology, by promoting kinesin-dependent apical migration of the centrosome and Golgi apparatus and basal localization of the nucleus (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Nesprin-4
Alternative name(s):
Nuclear envelope spectrin repeat protein 4
Gene namesi
Name:Syne4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1304580. Syne4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 291291CytoplasmicPROSITE-ProRule annotationAdd
BLAST
Transmembranei292 – 31221Helical; Anchor for type IV membrane proteinPROSITE-ProRule annotationAdd
BLAST
Topological domaini313 – 34028Perinuclear spacePROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Nesprin-4PRO_0000306266Add
BLAST

Proteomic databases

PaxDbiQ5M844.
PRIDEiQ5M844.

Expressioni

Gene expression databases

ExpressionAtlasiQ5M844. baseline and differential.
GenevisibleiQ5M844. RN.

Interactioni

Subunit structurei

Core component of the LINC complex which is composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN domain-containing proteins interact with A-type lamins of the nuclear lamina, while at the other end of the complex, nesprins interact with unique cytoskeletal components. Interacts with kinesins KIF5B and KLC1 (By similarity).By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini283 – 34058KASHPROSITE-ProRule annotationAdd
BLAST

Domaini

The KASH domain, which contains a transmembrane domain, mediates the nuclear envelope targeting and is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.By similarity

Sequence similaritiesi

Belongs to the nesprin family.Curated
Contains 1 KASH domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIPP. Eukaryota.
ENOG4111B09. LUCA.
GeneTreeiENSGT00510000049061.
HOGENOMiHOG000111992.
HOVERGENiHBG107763.
InParanoidiQ5M844.
OMAiSHRKHLA.
OrthoDBiEOG7NW69N.

Family and domain databases

InterProiIPR012315. KASH.
IPR030268. SYNE4.
[Graphical view]
PANTHERiPTHR21640:SF1. PTHR21640:SF1. 1 hit.
PfamiPF10541. KASH. 1 hit.
[Graphical view]
SMARTiSM01249. KASH. 1 hit.
[Graphical view]
PROSITEiPS51049. KASH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5M844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFPLLGHG FPPEPVNHPL GGPRGLDVAG PTICPAPEEE PSRPEQVQAS
60 70 80 90 100
LDAPEHFMDE PKSTESATSP SKLPLASSHE HQDGGKPCEA LQAELQGAAE
110 120 130 140 150
RVDALLVFGE GLAERSEPRA WTSLEQVLRA LGTHRDTIFQ RLWQLQAQLI
160 170 180 190 200
SYSLVLEKAN LLDQDLEVEG DSDGPAAGGV WGPWAPSIFP TPAELEWDPA
210 220 230 240 250
GDVGGLGPSG QKISRIPGAP CELCGYRGSQ SSGQGFEDLL SLGLGHRKHL
260 270 280 290 300
AAHHRRRLQK PQDKKRQGPP SLPDAMLEVD RGVPAPASRR PLTFLLLLLF
310 320 330 340
LLLVGATLLL PLSGVPCCSH TRLARTPYLV LSYVNGLPPI
Length:340
Mass (Da):36,515
Last modified:February 1, 2005 - v1
Checksum:i6A902E1B950B4346
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088237 mRNA. Translation: AAH88237.1.
RefSeqiNP_001009283.1. NM_001009283.1.
XP_003748869.1. XM_003748821.2.
UniGeneiRn.2550.

Genome annotation databases

EnsembliENSRNOT00000028265; ENSRNOP00000028265; ENSRNOG00000049277.
ENSRNOT00000051422; ENSRNOP00000044396; ENSRNOG00000020830.
GeneIDi100911963.
292781.
KEGGirno:100911963.
rno:292781.
UCSCiRGD:1304580. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088237 mRNA. Translation: AAH88237.1.
RefSeqiNP_001009283.1. NM_001009283.1.
XP_003748869.1. XM_003748821.2.
UniGeneiRn.2550.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ5M844.
PRIDEiQ5M844.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028265; ENSRNOP00000028265; ENSRNOG00000049277.
ENSRNOT00000051422; ENSRNOP00000044396; ENSRNOG00000020830.
GeneIDi100911963.
292781.
KEGGirno:100911963.
rno:292781.
UCSCiRGD:1304580. rat.

Organism-specific databases

CTDi163183.
RGDi1304580. Syne4.

Phylogenomic databases

eggNOGiENOG410IIPP. Eukaryota.
ENOG4111B09. LUCA.
GeneTreeiENSGT00510000049061.
HOGENOMiHOG000111992.
HOVERGENiHBG107763.
InParanoidiQ5M844.
OMAiSHRKHLA.
OrthoDBiEOG7NW69N.

Miscellaneous databases

NextBioi634781.
PROiQ5M844.

Gene expression databases

ExpressionAtlasiQ5M844. baseline and differential.
GenevisibleiQ5M844. RN.

Family and domain databases

InterProiIPR012315. KASH.
IPR030268. SYNE4.
[Graphical view]
PANTHERiPTHR21640:SF1. PTHR21640:SF1. 1 hit.
PfamiPF10541. KASH. 1 hit.
[Graphical view]
SMARTiSM01249. KASH. 1 hit.
[Graphical view]
PROSITEiPS51049. KASH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiSYNE4_RAT
AccessioniPrimary (citable) accession number: Q5M844
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: February 1, 2005
Last modified: May 11, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.