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Protein

Pirin

Gene

Pir

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro) (By similarity).By similarity

Catalytic activityi

Quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+.

Cofactori

Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity

Pathwayi: quercetin degradation

This protein is involved in the pathway quercetin degradation, which is part of Flavonoid metabolism.
View all proteins of this organism that are known to be involved in the pathway quercetin degradation and in Flavonoid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561IronBy similarity
Metal bindingi58 – 581IronBy similarity
Metal bindingi101 – 1011IronBy similarity
Metal bindingi103 – 1031IronBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00724.

Names & Taxonomyi

Protein namesi
Recommended name:
Pirin (EC:1.13.11.24)
Alternative name(s):
Probable quercetin 2,3-dioxygenase PIR
Short name:
Probable quercetinase
Gene namesi
Name:Pir
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi1359212. Pir.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Predominantly localized in dot-like subnuclear structures.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291PirinPRO_0000288474Add
BLAST

Proteomic databases

PaxDbiQ5M827.
PRIDEiQ5M827.

PTM databases

iPTMnetiQ5M827.

Expressioni

Gene expression databases

GenevisibleiQ5M827. RN.

Interactioni

Subunit structurei

May interact with NF1/CTF1. Interacts with BCL3. Identified in a complex comprised of PIR, BLC3, NFKB1 and target DNA (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004884.

Structurei

3D structure databases

ProteinModelPortaliQ5M827.
SMRiQ5M827. Positions 3-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pirin family.Curated

Phylogenomic databases

eggNOGiENOG410IF52. Eukaryota.
COG1741. LUCA.
GeneTreeiENSGT00390000008044.
HOGENOMiHOG000248360.
HOVERGENiHBG019151.
InParanoidiQ5M827.
KOiK06911.
OMAiDPFVHMD.
OrthoDBiEOG7JMGFH.
PhylomeDBiQ5M827.
TreeFamiTF300002.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR012093. Pirin.
IPR008778. Pirin_C_dom.
IPR003829. Pirin_N_dom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR13903. PTHR13903. 1 hit.
PfamiPF02678. Pirin. 1 hit.
PF05726. Pirin_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006232. Pirin. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5M827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSKKVTLS VLSREQSEGV GARVRRSIGG PELKMLDPFL LFDEFKGGRP
60 70 80 90 100
GGFPDHPHRG FETVSYLLEG GSMAHEDFCG HVGKMNPGDL QWMTAGRGIL
110 120 130 140 150
HAEMPCSEEP AHGLQLWVNL KRSEKMVEPQ YQELKSEEIP KPSKDGVTVA
160 170 180 190 200
VISGEALGIK SKVYTRTPTL YLDFKLDQGA KHSQPIPKGW TSFIYTISGD
210 220 230 240 250
VYIGPDDAQQ KIEPHRTAVL GEGDTVQLEN KDPKRSHFVL IAGEPLREPV
260 270 280 290
VQHGPFVMNT NEEISEAILD FRNAKNGFEG AKTWKSKIGN Q
Length:291
Mass (Da):32,179
Last modified:February 1, 2005 - v1
Checksum:iE2DD54C259D831F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088290 mRNA. Translation: AAH88290.1.
RefSeqiNP_001009474.1. NM_001009474.1.
UniGeneiRn.103685.

Genome annotation databases

EnsembliENSRNOT00000004884; ENSRNOP00000004884; ENSRNOG00000003674.
GeneIDi363465.
KEGGirno:363465.
UCSCiRGD:1359212. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088290 mRNA. Translation: AAH88290.1.
RefSeqiNP_001009474.1. NM_001009474.1.
UniGeneiRn.103685.

3D structure databases

ProteinModelPortaliQ5M827.
SMRiQ5M827. Positions 3-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004884.

PTM databases

iPTMnetiQ5M827.

Proteomic databases

PaxDbiQ5M827.
PRIDEiQ5M827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004884; ENSRNOP00000004884; ENSRNOG00000003674.
GeneIDi363465.
KEGGirno:363465.
UCSCiRGD:1359212. rat.

Organism-specific databases

CTDi8544.
RGDi1359212. Pir.

Phylogenomic databases

eggNOGiENOG410IF52. Eukaryota.
COG1741. LUCA.
GeneTreeiENSGT00390000008044.
HOGENOMiHOG000248360.
HOVERGENiHBG019151.
InParanoidiQ5M827.
KOiK06911.
OMAiDPFVHMD.
OrthoDBiEOG7JMGFH.
PhylomeDBiQ5M827.
TreeFamiTF300002.

Enzyme and pathway databases

UniPathwayiUPA00724.

Miscellaneous databases

PROiQ5M827.

Gene expression databases

GenevisibleiQ5M827. RN.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR012093. Pirin.
IPR008778. Pirin_C_dom.
IPR003829. Pirin_N_dom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR13903. PTHR13903. 1 hit.
PfamiPF02678. Pirin. 1 hit.
PF05726. Pirin_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006232. Pirin. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  2. Lubec G., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 35-46 AND 145-160, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiPIR_RAT
AccessioniPrimary (citable) accession number: Q5M827
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 1, 2005
Last modified: June 8, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.