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Protein

Microtubule-associated protein 4

Gene

Map4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Non-neuronal microtubule-associated protein. Promotes microtubule assembly (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein 4
Short name:
MAP-4
Gene namesi
Name:Map4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1564359. Map4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10571056Microtubule-associated protein 4PRO_0000323721Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei5 – 51PhosphoserineBy similarity
Modified residuei60 – 601PhosphoserineBy similarity
Modified residuei99 – 991PhosphoserineBy similarity
Modified residuei254 – 2541PhosphoserineBy similarity
Modified residuei257 – 2571PhosphoserineCombined sources
Modified residuei417 – 4171PhosphoserineBy similarity
Modified residuei419 – 4191PhosphothreonineBy similarity
Modified residuei450 – 4501PhosphothreonineBy similarity
Modified residuei497 – 4971PhosphothreonineBy similarity
Modified residuei506 – 5061PhosphothreonineBy similarity
Modified residuei509 – 5091PhosphoserineCombined sources
Modified residuei515 – 5151PhosphoserineCombined sources
Modified residuei520 – 5201PhosphoserineCombined sources
Modified residuei522 – 5221PhosphoserineCombined sources
Modified residuei546 – 5461PhosphothreonineBy similarity
Modified residuei599 – 5991PhosphoserineBy similarity
Modified residuei618 – 6181PhosphoserineCombined sources
Modified residuei659 – 6591PhosphothreonineCombined sources
Modified residuei668 – 6681PhosphoserineBy similarity
Modified residuei685 – 6851PhosphoserineBy similarity
Modified residuei695 – 6951PhosphoserineBy similarity
Modified residuei761 – 7611PhosphoserineBy similarity
Modified residuei799 – 7991PhosphoserineBy similarity
Modified residuei801 – 8011PhosphoserineBy similarity
Modified residuei827 – 8271PhosphoserineBy similarity
Modified residuei902 – 9021PhosphoserineCombined sources
Modified residuei915 – 9151PhosphoserineCombined sources
Modified residuei915 – 9151Phosphoserine; by MARK11 Publication
Modified residuei916 – 9161PhosphothreonineBy similarity
Modified residuei978 – 9781PhosphoserineCombined sources
Modified residuei1050 – 10501PhosphoserineBy similarity
Modified residuei1056 – 10561PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation on Ser-761 negatively regulates MAP4 activity to promote microtubule assembly. Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ5M7W5.

PTM databases

iPTMnetiQ5M7W5.
PhosphoSiteiQ5M7W5.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020748.
ExpressionAtlasiQ5M7W5. baseline and differential.
GenevisibleiQ5M7W5. RN.

Interactioni

Subunit structurei

Interacts with SEPT2; this interaction impedes tubulin-binding (By similarity). Interacts with TRAF3IP1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi266360. 1 interaction.
IntActiQ5M7W5. 1 interaction.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati897 – 92731Tau/MAP 1Add
BLAST
Repeati928 – 95831Tau/MAP 2Add
BLAST
Repeati959 – 99032Tau/MAP 3Add
BLAST

Sequence similaritiesi

Contains 3 Tau/MAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000063491.
HOGENOMiHOG000113478.
HOVERGENiHBG006323.
InParanoidiQ5M7W5.
KOiK10431.
PhylomeDBiQ5M7W5.

Family and domain databases

InterProiIPR027324. MAP2/MAP4/Tau.
IPR027323. MAP4.
IPR001084. MAP_tubulin-bd_rpt.
[Graphical view]
PANTHERiPTHR11501. PTHR11501. 1 hit.
PTHR11501:SF16. PTHR11501:SF16. 1 hit.
PfamiPF00418. Tubulin-binding. 3 hits.
[Graphical view]
PROSITEiPS00229. TAU_MAP_1. 3 hits.
PS51491. TAU_MAP_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5M7W5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADLSLVDAL TEPPPEIEGE IKRDFMAALE AEPYDDIVGE TVEKTEFIPL
60 70 80 90 100
LDGDEKSGNS ESKRKPCVDT SQVEDIPSSK PTLLANGDHG VEGNNTTGSP
110 120 130 140 150
TDFLVENVDY EDYQNSQSWP EDASFCFQPQ QVLDTNQADP FNVHHDDGLA
160 170 180 190 200
DLLFVSSGPT NASAFIEENN PLEDSYGVLP CDSFAPTAVV SQEWSVGAPD
210 220 230 240 250
SPHSEPCVSP EVTIETAQPA TELSKAVDLE SVKEQLPAKA LEMMAGQTTD
260 270 280 290 300
AVPSKESEGS PDTDAAPGPD TDVTLTKDIE ESSSPDVISA NVTQPFTESD
310 320 330 340 350
MFLTQEMELL IGTEAAQVKD TMSSVEPDIS SAKNTAPPTE EETVPGKDMT
360 370 380 390 400
FPKEAETALP IEMDLAPPED VALPKETELE LAPAAGTAPL SETEVALAKD
410 420 430 440 450
EEPSTGIPAA QEMLLSSETE VVLPSDSIMT LTKEVTVPLE AAGPLVSDMT
460 470 480 490 500
AILETEMTLG GGTATPTETK LGKVKDMAPL PESEVALGKD VVTLPETKVT
510 520 530 540 550
EFNNVTPLSE EEVASIKDVS PSPETETAKN ADLHSGTELT LDNSMTPPSD
560 570 580 590 600
PALPLETKVA TVQIKDKETV QTQEELSEDS QLESVQLEGQ SAVPPCTISP
610 620 630 640 650
EPVKAADQKS TLPVDEGSPL EKLEQKETSG SQPPELCSGV SRQEEGKAAV
660 670 680 690 700
GLTGNDIATP PNKELPPSPE KKAKPLATTQ PAKTSTSKAK IQPTSLPKQP
710 720 730 740 750
APTTSGGLNK KPMSLASGSV PAAPHKRPAA ATATARPSTL PARDLKPKPI
760 770 780 790 800
TETKVAEKRT SPSKPSSAPA LRPGPKTTPT ISKATSPSTL VSTGSSSRSP
810 820 830 840 850
STTLPKRPTT TKTEGKPADV KRMTAKSATA DLSRSKTTSA SSVKRNTTPT
860 870 880 890 900
GATPPAGMAS TRVKPMSAPC RSSVALSVDK KPTSTKPSSS APRVSRLATT
910 920 930 940 950
VSAPDLKSVR SKVGSTENMK HQPGGGRVQI QNKKVDISKV SSKCGSKANI
960 970 980 990 1000
KHKPGGGDVK IESQKLNFKE KAQAKVGSLD NVGHLPAGGT VKTEGGGSEA
1010 1020 1030 1040 1050
PPCPGPPAGE EPAIPEAAPD AGAPTSASGL SGHTTLSGGG DQREPQTLDS

QIQETSI
Length:1,057
Mass (Da):110,301
Last modified:February 1, 2005 - v1
Checksum:iC306932206B242CB
GO
Isoform 2 (identifier: Q5M7W5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     674-747: Missing.

Show »
Length:983
Mass (Da):102,840
Checksum:iA718DCE21B776850
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei674 – 74774Missing in isoform 2. 1 PublicationVSP_032080Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088405 mRNA. Translation: AAH88405.1.
AB175782 mRNA. Translation: BAD14292.1.
AB175783 mRNA. Translation: BAD14293.1.
RefSeqiNP_001019449.1. NM_001024278.1. [Q5M7W5-1]
UniGeneiRn.203122.

Genome annotation databases

EnsembliENSRNOT00000056166; ENSRNOP00000053011; ENSRNOG00000020748. [Q5M7W5-1]
GeneIDi367171.
KEGGirno:367171.
UCSCiRGD:1564359. rat. [Q5M7W5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088405 mRNA. Translation: AAH88405.1.
AB175782 mRNA. Translation: BAD14292.1.
AB175783 mRNA. Translation: BAD14293.1.
RefSeqiNP_001019449.1. NM_001024278.1. [Q5M7W5-1]
UniGeneiRn.203122.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi266360. 1 interaction.
IntActiQ5M7W5. 1 interaction.

PTM databases

iPTMnetiQ5M7W5.
PhosphoSiteiQ5M7W5.

Proteomic databases

PRIDEiQ5M7W5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000056166; ENSRNOP00000053011; ENSRNOG00000020748. [Q5M7W5-1]
GeneIDi367171.
KEGGirno:367171.
UCSCiRGD:1564359. rat. [Q5M7W5-1]

Organism-specific databases

CTDi4134.
RGDi1564359. Map4.

Phylogenomic databases

GeneTreeiENSGT00530000063491.
HOGENOMiHOG000113478.
HOVERGENiHBG006323.
InParanoidiQ5M7W5.
KOiK10431.
PhylomeDBiQ5M7W5.

Miscellaneous databases

PROiQ5M7W5.

Gene expression databases

BgeeiENSRNOG00000020748.
ExpressionAtlasiQ5M7W5. baseline and differential.
GenevisibleiQ5M7W5. RN.

Family and domain databases

InterProiIPR027324. MAP2/MAP4/Tau.
IPR027323. MAP4.
IPR001084. MAP_tubulin-bd_rpt.
[Graphical view]
PANTHERiPTHR11501. PTHR11501. 1 hit.
PTHR11501:SF16. PTHR11501:SF16. 1 hit.
PfamiPF00418. Tubulin-binding. 3 hits.
[Graphical view]
PROSITEiPS00229. TAU_MAP_1. 3 hits.
PS51491. TAU_MAP_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAP4_RAT
AccessioniPrimary (citable) accession number: Q5M7W5
Secondary accession number(s): Q75NR5, Q75NR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.