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Protein

Thioredoxin-interacting protein

Gene

Txnip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular response to tumor cell Source: UniProtKB
  • keratinocyte differentiation Source: Ensembl
  • negative regulation of cell division Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • platelet-derived growth factor receptor signaling pathway Source: Ensembl
  • positive regulation of apoptotic process Source: RGD
  • protein import into nucleus Source: Ensembl
  • regulation of cell proliferation Source: RGD
  • response to calcium ion Source: RGD
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to glucose Source: RGD
  • response to hydrogen peroxide Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to progesterone Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-844456. The NLRP3 inflammasome.

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin-interacting protein
Alternative name(s):
Vitamin D3 up-regulated protein 1
Gene namesi
Name:Txnip
Synonyms:Vdup1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi620886. Txnip.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • mitochondrial intermembrane space Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Thioredoxin-interacting proteinPRO_0000250493Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi63 – 63InterchainBy similarity
Cross-linki212 – 212Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei361 – 3611PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.By similarity

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5M7W1.
PRIDEiQ5M7W1.

Expressioni

Gene expression databases

GenevisibleiQ5M7W1. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1 (By similarity). Interacts (via C-terminus) with ITCH (via WW domains). Interacts with DDIT4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi250736. 1 interaction.
IntActiQ5M7W1. 1 interaction.
STRINGi10116.ENSRNOP00000028793.

Structurei

3D structure databases

ProteinModelPortaliQ5M7W1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arrestin family.Curated

Phylogenomic databases

eggNOGiKOG3780. Eukaryota.
ENOG41102NY. LUCA.
GeneTreeiENSGT00550000074356.
HOGENOMiHOG000237328.
HOVERGENiHBG066469.
InParanoidiQ5M7W1.
OMAiLEDQPTG.
OrthoDBiEOG7P02HT.
PhylomeDBiQ5M7W1.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5M7W1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVMFKKIKSF EVVFNDPEKV YGSGEKVAGR VTVEVCEVTR VKAVRILACG
60 70 80 90 100
VAKVLWMQGS QQCKQTLDYL RYEDTLLLED QPTGENEMVI MRPGNKYEYK
110 120 130 140 150
FGFELPQGPL GTSFKGKYGC VDYWVKAFLD RPSQPTQEAK KNFEVMDLVD
160 170 180 190 200
VNTPDLMAPV SAKKEKKVSC MFIPDGRVSV SARIDRKGFC EGDDISIHAD
210 220 230 240 250
FENTCSRIVV PKAAIVARHT YLANGQTKVL TQKLSSVRGN HIISGTCASW
260 270 280 290 300
RGKSLRVQKI RPSILGCNIL RVEYSLLIYV SVPGSKKVIL DLPLVIGSRS
310 320 330 340 350
GLSSRTSSMA SRTSSEMSWI DLNIPDTPEA PPCYMDVIPE DHRLESPTTP
360 370 380 390
LLDDVDDSQD SPIFMYAPEF QFMPPPTYTE VDPCVLNNNN NNVQ
Length:394
Mass (Da):44,018
Last modified:February 1, 2005 - v1
Checksum:i6CE164C68D4B0CBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088411 mRNA. Translation: AAH88411.1.
RefSeqiNP_001008767.1. NM_001008767.1.
UniGeneiRn.2758.

Genome annotation databases

EnsembliENSRNOT00000028793; ENSRNOP00000028793; ENSRNOG00000021201.
GeneIDi117514.
KEGGirno:117514.
UCSCiRGD:620886. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088411 mRNA. Translation: AAH88411.1.
RefSeqiNP_001008767.1. NM_001008767.1.
UniGeneiRn.2758.

3D structure databases

ProteinModelPortaliQ5M7W1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250736. 1 interaction.
IntActiQ5M7W1. 1 interaction.
STRINGi10116.ENSRNOP00000028793.

Proteomic databases

PaxDbiQ5M7W1.
PRIDEiQ5M7W1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028793; ENSRNOP00000028793; ENSRNOG00000021201.
GeneIDi117514.
KEGGirno:117514.
UCSCiRGD:620886. rat.

Organism-specific databases

CTDi10628.
RGDi620886. Txnip.

Phylogenomic databases

eggNOGiKOG3780. Eukaryota.
ENOG41102NY. LUCA.
GeneTreeiENSGT00550000074356.
HOGENOMiHOG000237328.
HOVERGENiHBG066469.
InParanoidiQ5M7W1.
OMAiLEDQPTG.
OrthoDBiEOG7P02HT.
PhylomeDBiQ5M7W1.

Enzyme and pathway databases

ReactomeiR-RNO-844456. The NLRP3 inflammasome.

Miscellaneous databases

NextBioi620265.
PROiQ5M7W1.

Gene expression databases

GenevisibleiQ5M7W1. RN.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiTXNIP_RAT
AccessioniPrimary (citable) accession number: Q5M7W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: February 1, 2005
Last modified: November 11, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.