Q5M7N8 (SYCC_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase, cytoplasmic
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:

cars

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 747	747	Cysteine--tRNA ligase, cytoplasmic		PRO_0000250747
Regions
	Motif	56 – 66	11	"HIGH" region
	Motif	405 – 409	5	"KMSKS" region
Sites
	Metal binding	54	1	Zinc By similarity
	Metal binding	347	1	Zinc By similarity
	Metal binding	372	1	Zinc By similarity
	Metal binding	376	1	Zinc By similarity
	Binding site	408	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5M7N8 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 27E4CEDCD43FB970	FASTA	747	85,477
	10 20 30 40 50 60 MTDSWERGKG RRTQPPWSAP NTQAQPGLRL YNSLTRSKEL FVPQDGNKVT WYCCGPTVYD 70 80 90 100 110 120 ASHMGHARSY ISFDILRRVL RDYFKYDVFY CMNITDIDDK IIKRARQRHL FQQYRERNPR 130 140 150 160 170 180 PSDLLQDVSA ALTPFLQRIS EANDPDKRQM LERIHGSVSA ALLPLQDAVS SNARAEELER 190 200 210 220 230 240 LSQELMEAAV DLLSDWLDEK HGAQITDNSI FSQLPKHWES EYHRDMEALN VLPPDVLTRV 250 260 270 280 290 300 SEYVPEIVAF VQRIVDNGYG YVSNGSVYFS TAKFHASEKH YYAKLVPEAV GDQKALQEGE 310 320 330 340 350 360 GDLSISADRL SEKQSPNDFA LWKASKPGEP SWESPWGKGR PGWHIECSAM AGSILGESMD 370 380 390 400 410 420 IHGGGFDLRF PHHDNELAQS EAYFDNDHWV RYFLHTGHLT IAGCKMSKSL KNFITIKDAL 430 440 450 460 470 480 QKNTARQLRL AFLMHAWKDT LDYSSNTMES AVQYEKFMNE FFLNVKDLLR APTDVTGQFV 490 500 510 520 530 540 KWEVPELELN SCFYSKKAAV HEALCDNIDT RTVMEEMRSL VSQCNSYIAS RKAAKQPPNR 550 560 570 580 590 600 LLLRSVSSYL TAMLKVFGAI EGEEVIGFPI GGSDNSMNLE STVMPYLQVL SQFREGVRQI 610 620 630 640 650 660 ARQHKVTEVL QLSDLLRDDV LPELGVRLED HEGLPTVVKL VDRETLLKEK EEKRKAEEEK 670 680 690 700 710 720 QRKKEEAARK KQQQEAAKLE KMKVSPSQMF QLETDKYSQF DESGFPTHDT EGKELSKGQS 730 740 KKLRKLYEAQ EKLHKEYLQM AQNGTTG

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC088531 mRNA. Translation: AAH88531.1.
RefSeq	NP_001011365.1. NM_001011365.1.
UniGene	Str.11779.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ProteinModelPortal	Q5M7N8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5M7N8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	496832.
KEGG	xtr:496832.
Organism-specific databases
CTD	833.
Xenbase	XB-GENE-5825933. cars.
Phylogenomic databases
eggNOG	veNOG07440.
GeneTree	ENSGT00390000006347.
HOVERGEN	HBG002089.
Family and domain databases
InterPro	IPR015803. Cys-tRNA-synt. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCC_XENTR

Accession

Primary (citable) accession number: Q5M7N8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program