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Protein

Probable protein S-acyltransferase 12

Gene

PAT12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Palmitoyl acyltransferase.By similarity1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei141S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein S-acyltransferase 12 (EC:2.3.1.225)
Alternative name(s):
Probable palmitoyltransferase At4g00840
Zinc finger DHHC domain-containing protein At4g00840
Gene namesi
Name:PAT12
Ordered Locus Names:At4g00840
ORF Names:A_TM018A10.8, T18A10.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G00840.
TAIRilocus:2134643. AT4G00840.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003636011 – 291Probable protein S-acyltransferase 12Add BLAST291

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ5M757.

Expressioni

Gene expression databases

GenevisibleiQ5M757. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G00840.1.

Structurei

3D structure databases

ProteinModelPortaliQ5M757.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 161DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1315. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000234777.
InParanoidiQ5M757.
KOiK20028.
OMAiGLRCCQR.
OrthoDBiEOG09360KT0.
PhylomeDBiQ5M757.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5M757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLFRFCSGL KVLGYFMILL VVAVVGVSYY AVVVSTWWPI LIRGDHGALS
60 70 80 90 100
ALAALIIFVF HFLLIMLLWS YFTTVFTDPG SVPEHFRREM GGGDSLEAGT
110 120 130 140 150
STDQGAFGSL GYCTKCRNVK PPRCHHCSVC QRCVLKMDHH CVWIVNCVGA
160 170 180 190 200
RNYKFFLLFL FYTFLETMLD VIVLLPSFIE FFSQAIKHSS SPGKLASLVL
210 220 230 240 250
AFVLNFAFVL SLLCFVVMHI SLLSSNTTSV EVHEKNGEVR WKYDLGKKKN
260 270 280 290
FEQVFGKKKA FWLLPLYSKD DIDNITSLEG LEFPTCSDID P
Length:291
Mass (Da):32,866
Last modified:February 1, 2005 - v1
Checksum:i3AF01A5B3FE08378
GO

Sequence cautioni

The sequence AAB62854 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80893 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013294 Genomic DNA. Translation: AAB62854.1. Sequence problems.
AL161472 Genomic DNA. Translation: CAB80893.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE81944.1.
BT020391 mRNA. Translation: AAV91337.1.
BT020511 mRNA. Translation: AAW39012.1.
AK229293 mRNA. Translation: BAF01156.1.
PIRiT01562.
RefSeqiNP_567193.2. NM_116310.5.
UniGeneiAt.24279.
At.71106.

Genome annotation databases

EnsemblPlantsiAT4G00840.1; AT4G00840.1; AT4G00840.
GeneIDi826193.
GrameneiAT4G00840.1; AT4G00840.1; AT4G00840.
KEGGiath:AT4G00840.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiZDH15_ARATH
AccessioniPrimary (citable) accession number: Q5M757
Secondary accession number(s): O23092
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 1, 2005
Last modified: June 7, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families