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Protein

Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial

Gene

At1g54220

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).1 Publication

Catalytic activityi

Acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine.

Cofactori

(R)-lipoateNote: Binds 1 lipoyl cofactor covalently.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei512Sequence analysis1
Active sitei516Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycolysis

Enzyme and pathway databases

BioCyciARA:AT1G54220-MONOMER.
ReactomeiR-ATH-204174. Regulation of pyruvate dehydrogenase (PDH) complex.
R-ATH-5362517. Signaling by Retinoic Acid.
R-ATH-70268. Pyruvate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial (EC:2.3.1.12)
Alternative name(s):
Dihydrolipoamide S-acetyltransferase component 3 of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2 3
Short name:
PDC-E2 3
Short name:
PDCE2 3
Gene namesi
Ordered Locus Names:At1g54220
ORF Names:F20D21.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G54220.
TAIRilocus:2020173. AT1G54220.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 102Mitochondrion1 PublicationAdd BLAST102
ChainiPRO_0000260027103 – 539Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrialAdd BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

PaxDbiQ5M729.
PRIDEiQ5M729.

Expressioni

Gene expression databases

ExpressionAtlasiQ5M729. baseline and differential.
GenevisibleiQ5M729. AT.

Interactioni

Protein-protein interaction databases

BioGridi27088. 1 interactor.
STRINGi3702.AT1G54220.1.

Structurei

3D structure databases

ProteinModelPortaliQ5M729.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 187Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST77
Domaini248 – 285Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST38

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG0557. Eukaryota.
COG0508. LUCA.
HOGENOMiHOG000281566.
InParanoidiQ5M729.
KOiK00627.
OMAiAIMEFES.
OrthoDBiEOG093607WT.
PhylomeDBiQ5M729.

Family and domain databases

Gene3Di4.10.320.10. 1 hit.
InterProiView protein in InterPro
IPR003016. 2-oxoA_DH_lipoyl-BS.
IPR001078. 2-oxoacid_DH_actylTfrase.
IPR000089. Biotin_lipoyl.
IPR004167. E3-bd.
IPR006257. LAT1.
IPR011053. Single_hybrid_motif.
PfamiView protein in Pfam
PF00198. 2-oxoacid_dh. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02817. E3_binding. 1 hit.
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR01349. PDHac_trf_mito. 1 hit.
PROSITEiView protein in PROSITE
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
PS51826. PSBD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5M729-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYASRIINH SKKLKDVSTL LRRENAATIR YYSNTNRAPL NREDTFNSRL
60 70 80 90 100
GYPPLERISI CSTSTLPVSI IFSTTRSNLS SAMGRPIFGK EFSCLMQSAR
110 120 130 140 150
GFSSGSDLPP HQEIGMPSLS PTMTEGNIAR WLKKEGDKVA PGEVLCEVET
160 170 180 190 200
DKATVEMECM EEGYLAKIVK AEGSKEIQVG EVIAITVEDE EDIGKFKDYT
210 220 230 240 250
PSSTADAAPT KAEPTPAPPK EEKVKQPSSP PEPKASKPST PPTGDRVFAS
260 270 280 290 300
PLARKLAEDN NVPLSDIEGT GPEGRIVKAD IDEYLASSGK GATAKPSKST
310 320 330 340 350
DSKAPALDYV DIPHSQIRKV TASRLAFSKQ TIPHYYLTVD TCVDKLMALR
360 370 380 390 400
SQLNSFKEAS GGKRISVNDL VVKAAALALR KVPQCNSSWT DDYIRQFKNV
410 420 430 440 450
NINVAVQTEN GLYVPVVKDA DRKGLSTIGE EVRLLAQKAK ENSLKPEDYE
460 470 480 490 500
GGTFTVSNLG GPFGIKQFCA VVNPPQAAIL AVGSAEKRVV PGNGPDQFNF
510 520 530
ASYMPVTLSC DHRVVDGAIG AEWLKAFKGY IENPKSMLL
Length:539
Mass (Da):58,467
Last modified:February 1, 2005 - v1
Checksum:i0C4E141079A2698F
GO

Sequence cautioni

The sequence AAD25602 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45T → I in AAK53067 (Ref. 1) Curated1
Sequence conflicti267I → T in AAK53067 (Ref. 1) Curated1
Sequence conflicti442N → S in AAM97076 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY033001 mRNA. Translation: AAK53067.1.
AC005287 Genomic DNA. Translation: AAD25602.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33067.1.
CP002684 Genomic DNA. Translation: AEE33068.1.
AY136410 mRNA. Translation: AAM97076.1.
BT020419 mRNA. Translation: AAV97810.1.
PIRiE96583.
RefSeqiNP_001031186.1. NM_001036109.1.
NP_564654.1. NM_104300.4.
UniGeneiAt.19093.
At.21338.

Genome annotation databases

EnsemblPlantsiAT1G54220.1; AT1G54220.1; AT1G54220.
AT1G54220.2; AT1G54220.2; AT1G54220.
GeneIDi841863.
GrameneiAT1G54220.1; AT1G54220.1; AT1G54220.
AT1G54220.2; AT1G54220.2; AT1G54220.
KEGGiath:AT1G54220.

Similar proteinsi

Entry informationi

Entry nameiODP23_ARATH
AccessioniPrimary (citable) accession number: Q5M729
Secondary accession number(s): Q8L787, Q94IP5, Q9SLL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: February 1, 2005
Last modified: August 30, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families