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Protein

Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial

Gene

At1g54220

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).1 Publication

Catalytic activityi

Acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine.

Cofactori

(R)-lipoateNote: Binds 1 lipoyl cofactor covalently.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei512Sequence analysis1
Active sitei516Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycolysis

Enzyme and pathway databases

BioCyciARA:AT1G54220-MONOMER
ReactomeiR-ATH-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-ATH-5362517 Signaling by Retinoic Acid
R-ATH-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial (EC:2.3.1.12)
Alternative name(s):
Dihydrolipoamide S-acetyltransferase component 3 of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2 3
Short name:
PDC-E2 3
Short name:
PDCE2 3
Gene namesi
Ordered Locus Names:At1g54220
ORF Names:F20D21.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G54220
TAIRilocus:2020173 AT1G54220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 102Mitochondrion1 PublicationAdd BLAST102
ChainiPRO_0000260027103 – 539Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrialAdd BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

PaxDbiQ5M729
PRIDEiQ5M729

Expressioni

Gene expression databases

ExpressionAtlasiQ5M729 baseline and differential
GenevisibleiQ5M729 AT

Interactioni

Protein-protein interaction databases

BioGridi270881 interactor.
STRINGi3702.AT1G54220.1

Structurei

3D structure databases

ProteinModelPortaliQ5M729
SMRiQ5M729
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 187Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST77
Domaini248 – 285Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST38

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG0557 Eukaryota
COG0508 LUCA
HOGENOMiHOG000281566
InParanoidiQ5M729
KOiK00627
OMAiLEMPKWG
OrthoDBiEOG093607WT
PhylomeDBiQ5M729

Family and domain databases

Gene3Di3.30.559.101 hit
4.10.320.101 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR001078 2-oxoacid_DH_actylTfrase
IPR000089 Biotin_lipoyl
IPR023213 CAT-like_dom_sf
IPR036625 E3-bd_dom_sf
IPR006257 LAT1
IPR004167 PSBD
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF00198 2-oxoacid_dh, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02817 E3_binding, 1 hit
SUPFAMiSSF47005 SSF47005, 1 hit
SSF51230 SSF51230, 1 hit
TIGRFAMsiTIGR01349 PDHac_trf_mito, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00189 LIPOYL, 1 hit
PS51826 PSBD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5M729-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYASRIINH SKKLKDVSTL LRRENAATIR YYSNTNRAPL NREDTFNSRL
60 70 80 90 100
GYPPLERISI CSTSTLPVSI IFSTTRSNLS SAMGRPIFGK EFSCLMQSAR
110 120 130 140 150
GFSSGSDLPP HQEIGMPSLS PTMTEGNIAR WLKKEGDKVA PGEVLCEVET
160 170 180 190 200
DKATVEMECM EEGYLAKIVK AEGSKEIQVG EVIAITVEDE EDIGKFKDYT
210 220 230 240 250
PSSTADAAPT KAEPTPAPPK EEKVKQPSSP PEPKASKPST PPTGDRVFAS
260 270 280 290 300
PLARKLAEDN NVPLSDIEGT GPEGRIVKAD IDEYLASSGK GATAKPSKST
310 320 330 340 350
DSKAPALDYV DIPHSQIRKV TASRLAFSKQ TIPHYYLTVD TCVDKLMALR
360 370 380 390 400
SQLNSFKEAS GGKRISVNDL VVKAAALALR KVPQCNSSWT DDYIRQFKNV
410 420 430 440 450
NINVAVQTEN GLYVPVVKDA DRKGLSTIGE EVRLLAQKAK ENSLKPEDYE
460 470 480 490 500
GGTFTVSNLG GPFGIKQFCA VVNPPQAAIL AVGSAEKRVV PGNGPDQFNF
510 520 530
ASYMPVTLSC DHRVVDGAIG AEWLKAFKGY IENPKSMLL
Length:539
Mass (Da):58,467
Last modified:February 1, 2005 - v1
Checksum:i0C4E141079A2698F
GO

Sequence cautioni

The sequence AAD25602 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45T → I in AAK53067 (Ref. 1) Curated1
Sequence conflicti267I → T in AAK53067 (Ref. 1) Curated1
Sequence conflicti442N → S in AAM97076 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY033001 mRNA Translation: AAK53067.1
AC005287 Genomic DNA Translation: AAD25602.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33067.1
CP002684 Genomic DNA Translation: AEE33068.1
AY136410 mRNA Translation: AAM97076.1
BT020419 mRNA Translation: AAV97810.1
PIRiE96583
RefSeqiNP_001031186.1, NM_001036109.1
NP_564654.1, NM_104300.4
UniGeneiAt.19093
At.21338

Genome annotation databases

EnsemblPlantsiAT1G54220.1; AT1G54220.1; AT1G54220
AT1G54220.2; AT1G54220.2; AT1G54220
GeneIDi841863
GrameneiAT1G54220.1; AT1G54220.1; AT1G54220
AT1G54220.2; AT1G54220.2; AT1G54220
KEGGiath:AT1G54220

Similar proteinsi

Entry informationi

Entry nameiODP23_ARATH
AccessioniPrimary (citable) accession number: Q5M729
Secondary accession number(s): Q8L787, Q94IP5, Q9SLL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: February 1, 2005
Last modified: April 25, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome