Q5M6J5 (PUR5_STRT2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	stu0033

Organism

Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) [Complete proteome] [HAMAP]

Taxonomic identifier

264199 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus thermophilus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_0000258418

Sequences

	Sequence		Length	Mass (Da)
	Q5M6J5 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 8DF0565D886518B7	FASTA	339	36,337
	10 20 30 40 50 60 MSKNAYAQSG VDVEAGYEVV ERIKKHVART ERAGVMGGLG GFGGMFDLSK TGVKEPVLIS 70 80 90 100 110 120 GTDGVGTKLM LAIKYDKHDT IGQDCVAMCV NDIIAAGAEP LYFLDYVATG KNEPAKLEQV 130 140 150 160 170 180 IAGVAEGCVQ SGVALIGGET AEMPGMYGED DYDLAGFAVG VAEKSQIIDG SKVAEGDVLL 190 200 210 220 230 240 GLASSGIHSN GYSLVRRVFA DYTGEEVLPE LEGQKLKDVL LEPTRIYVKA ALPLIKEELV 250 260 270 280 290 300 NGIAHITGGG FIENVPRMFS DDLAAEIDES KVPVLPIFKA LEKYGEIKHE EMFEIFNMGI 310 320 330 GLMFAVKPEN VERVKELLDE PVYEIGRIVK KDGASVVIK

References

[1]

"Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus." Bolotin A., Quinquis B., Renault P., Sorokin A., Ehrlich S.D., Kulakauskas S., Lapidus A., Goltsman E., Mazur M., Pusch G.D., Fonstein M., Overbeek R., Kyprides N., Purnelle B., Prozzi D., Ngui K., Masuy D., Hancy F. , Burteau S., Boutry M., Delcour J., Goffeau A., Hols P. Nat. Biotechnol. 22:1554-1558(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-250 / LMG 18311.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000023 Genomic DNA. Translation: AAV59763.1.
RefSeq	YP_138578.1. NC_006448.1.
3D structure databases
HSSP	HSSP built from PDB template 1CLI based on UniProtKB P08178.
ProteinModelPortal	Q5M6J5.
SMR	Q5M6J5. Positions 14-335.
ModBase	Search...
Protein-protein interaction databases
STRING	264199.stu0033.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV59763; AAV59763; stu0033.
GeneID	3163831.
KEGG	stl:stu0033.
PATRIC	19801556. VBIStrThe97850_0035.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229090.
KO	K01933.
OMA	IDMIAMN.
ProtClustDB	PRK05385.
Enzyme and pathway databases
UniPathway	UPA00074; UER00129.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_STRT2

Accession

Primary (citable) accession number: Q5M6J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program